Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Pilot Scale Production of Poly (3-Hydroxybutyrate-co-3-hydroxy-valerate) by Fed-batch Culture of Recombinant Escherichia coli

  • Park, Jong-il (Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center) ;
  • Lee, Sang-Yup (Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Center for Vltramicrochemical Process Systems, Korea Advanced Institute of Science and Technology) ;
  • Kyungsup Shin (Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center) ;
  • Lee, Woo-Gi (Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center) ;
  • Park, Si-Jae (Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center) ;
  • Chang, Ho-Nam (Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center) ;
  • Chang, Yong-Keun (Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)［P(3HB/V)］, by fed-batch culture of recombinant Escherichia coli harboring a plasmid containing the Alcaligenes latus polyhy-droxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only, In a 30 L fermentor having a XLa value of 0.11 S­$^1$, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having a XLa of 0.03 S­$^1$, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively giving a productivity of 1.06g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinant E. coli in a large-scale fermentor having low KLa value.

Keywords

References

  1. Biotechnol. Bioeng. v.49 Bacterial polyhydroxyalkanoates. Lee, S. Y. https://doi.org/10.1002/(SICI)1097-0290(19960105)49:1<1::AID-BIT1>3.0.CO;2-P
  2. Trends Biotechnol. v.16 Bacterial and other biological systems for polyester production. Steinbuchel, A.;B. Fuchtenbusch https://doi.org/10.1016/S0167-7799(98)01194-9
  3. Trends Biotechnol. v.14 Plastic bacteria Progress and prospects for polyhydroxyalkanoate production in bacteria. Lee, S. Y. https://doi.org/10.1016/0167-7799(96)10061-5
  4. Bioprocess Eng. v.17 Process analysis and economic evaluation for poly(3-hydroxybutyrate) production by fermentation. Choi, J.;S. Y. Lee https://doi.org/10.1007/s004490050394
  5. Appl. Environ. Microbiol. v.64 Cloning of the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of these genes for enhanced production of poly(3-hydroxybutyrate) by recombinant Escherichia coli. Choi, J.;S. Y. Lee;K. Han
  6. Appl. Environ. Microbiol. v.65 High level production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by fedbatch culture of recombinant Escherichia coli. Choi, J.;S. Y. Lee
  7. Eur. J. Appl. Microbiol. Biotechnol. v.6 A rapid gas chromatographic method for the determination of poly--hydroxybutyric acid in microbial biomass. Braunegg, G.;B. Sonnleitner;R. M. Lafferty https://doi.org/10.1007/BF00500854
  8. Biotechnol. Bioeng. v.34 Measurement of KLa by a gassing-in method with oxygenenriched air Chang, H. N.;B. Halard;M.-Y. Murray https://doi.org/10.1002/bit.260340905
  9. Biopolymers. v.3a Fermentative production of SCL-PHAs. Lee, S. Y.;S. J. Park;Y. Doi(ed.);A. Steinbuchel(ed.)
  10. Biopolymers. v.3a Biosynthesis and fermentative production of SCL-MCL-PHAs. Lee, S. Y.;S. J. Park;Y. Doi(ed.);A. Steinbuchel(ed.)
  11. Chem. Eng. Sci. v.26 Mass transfer in mechanically agitated gas-liquid contactors. Mehta, V. D.;M. M. Sharma https://doi.org/10.1016/0009-2509(71)83019-1
  12. Biotechnol. Bioeng. v.74 Metabolic and kinetic analysis of poly(3-hydroxybutyrate) production by recombinant Escherichia coli. van Wegen, R. J.;S. Y. Lee;A. P. J. Middelberg https://doi.org/10.1002/bit.1096
  13. J. Microbiol. Biotechnol. v.9 Metabolic analysis of poly(3-hydroxybutyrate) production by recombinant Escherichia coli. Wong, H. H.;R. J. van Wegen;J. Choi;S. Y. Lee;A. P. J. Middelberg
  14. Biotechnol. Lett. v.24 Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pilot-scale fermenter. Park, S. J.;J. P. Park;S. Y. Lee https://doi.org/10.1023/A:1014196906095