생명과학회지 (Journal of Life Science)

  • 제12권6호
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  • Pages.821-834
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  • 2002
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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ITS 부위에 근거한 한국산 Alexandrium tamarense 5 클론의 계통분류학적 위치

Phylogenetic position of five Korean strains of Alexandrium tamarense(Dinophyceae), based on internal transcribed spacers ITS1 and ITS2 including nuclear-encoded 5.85 rRNA gene sequences

  • 조은섭 (국립수산진흥원 적조생물과) ;
  • 이삼근 (국립수산진흥원 적조생물과) ;
  • 김익수 (농업과학기술원 잠사곤충부)
  • Cho, Eun-Seob (Harmful Algal Research Division, National Fisheries Research and Development Institute) ;
  • Lee, Sam-Geun (Harmful Algal Research Division, National Fisheries Research and Development Institute) ;
  • Kim, Ik-Soo (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology)
  • 발행 : 2002.12.01
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초록

알렉산드륨 적조생물의 리보소옴 알엔에이 유전자의 ITS1, 2 및 5.8S부위를 대상으로 종간 혹은 종내의 유전적 다양도를 조사하기 위하여 지리적으로 격리된 33 스트레인 유전자의 염기서열를 비교했다. 진해만에서 분리된 AT-2, AT-6, AT-10, AT-A, AT-B 5클론은 일본종 OFX151-A과 동일한 유전자임을 발견했다. ITS부위에서 가장 짧은 종은 A. margalefi로 481 bp이며 가장 긴 종은 A. affine으로 528 bp로 나타났다. ITS1과 ITS2 염기서열에 대한 상호관계는 역으로 나타낸 반면에, G＋C 함량에 대한 상호관계는 플러스로 나타났다. 유전적 변이율은 0.3% (1 bp)에서 53% (305 bp)였다. A. tamarense과 가장 적게 유전적 변이율을 보인 종은 A. fundyense(1.2-2.3% ＝ 6-12 bp)인 반면에, A. catenella와는 큰 변이율 (19.8% ＝ 102 bp)을 보였고, A. catenella와 A. fundyense은 19.7% 상이하였다. 알렉산드륨 적조생물의 bootstrap은 약하게 지지되는 데도 불구하고, A. catenella 분리종은 독립적인 그룹으로 형성하여 상호간에는 강력한 bootstrap 값은 PAUP과 NJ 분석에서 보였다. A. cohorticula와 A. frateculus 적조생물은 항상 sub-group 내에서 높은 bootstrap을 가졌다. 결론적으로 ITS부위의 염기서열 분석은 알렉산드륨 적조생물의 집단내 혹은 집단간의 계통분류을 밝히는데 유용한 것으로 보였다.

In order to measure the inter- and intraspecific genetic divergences within the genus Alexandrium, the variations within the internal transcribed spacer (ITS1 and ITS2) regions and 5.85 ribosomal RNA gene of eight Alexandrium species were examined for 33 strains from diverse geographical locations by direct sequencing. Five isolates of A. tamarense (AT-2, AT-6, AT-10, AT-A and AT-B) from Jinhae Bay, Korea were found to be completely identical to a Japanese strain OFX151-A. The length of the amplified ITSI-5.85-ITS2 region varied from 481 nucleotides (in A. margalefi) to 528 nucleotides (in A. affine CU1-1). ITS1 and ITS2 nucleotide lengths were negatively correlated, whereas a positive correlation was found between their G＋C content. The degree of sequence divergence ranged from 0.3% (1 bp) to a maximum of 53% (305 Up). Pairwise sequence comparisons revealed a small degree of divergence between A. tamarense and A. Pundyense isolates (1.2 - 2.3% ＝ 6-12 bp), but a high degree of divergence between A. tamarense and A. catenella (19.8% ＝ 102 bp), and between A. catenella and A. Pundyense (19.7%). Although most nodes were weakly supported by bootstrap values, some types tend to form independent molecular groups. A. catenella isolates also formed an independent molecular sub-group, with relaticula strong bootstrap values (94% or 85% and 79% or 98%, respectively in PAUP and NJ trees). Interestingly, A. cohorticula and A. frateculus always clustered within the same sub-group, this result being supported by strong bootstrap values. Our results indicate that the ITS regions provide useful informations on hierarchical population genetic structure and a high phylogenetic resolution in intraspecific and interspecific Alexandrium population.

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