Low-Dose Gamma Irradiation as Means of Isolating Carotenoid-Hyperproducing Yeast Mutant

  • Sun, Nam-Kyu (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Lee, Seung-Hee (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Ahn, Gil-Hwan (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Won, Mi-Sun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Song, Kyung-Bin (Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University)
  • Published : 2002.12.01

Abstract

In order to isolate carotenoid-hyperproducing yeast, low-dose gamma irradiation was used as means of mutagenesis. Phaffia rhodozyma was treated by gamma irradiation of less than 10 kGy, which is considered to be a wholesome irradiation condition established by the Food and Drug Administration. Through repeated rounds of gamma irradiation and visual screening, mutant 3A4-8 was obtained. It produced a $3,824{\mu}g$ carotenoid/g yeast, 69% higher content than $2,265{\mu}g/g$ yeast of the unirradiated one. This result indicates that low-dose gamma irradiation could be used as means of mutagenesis to obtain carotenoid-hyperproducing strain of Phaffia rhodozyma, since only carotenoid-hyperproducing yeast survived gamma irradiation by scavenging oxygen radicals generated by radiolysis of water.

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