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Physiological and Morphological Aspects of Bipolaris sorokiniana Conidia Surviving on Wheat Straw

  • Duveiller, E. (Department of Mycology and Plant Pathology, Banaras Hindu University) ;
  • Chand, R. (Department of Mycology and Plant Pathology, Banaras Hindu University) ;
  • Singh, H.V. (Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University) ;
  • Joshi, A.K. (CIMMYT South Asia Regional Program)
  • Published : 2002.12.01

Abstract

Wheat samples showing typical spot blotch symptoms on stems and sheaths were collected from the field after physiological maturity, and were sealed in paper bags and stored in the laboratory at room temperature to study the survival of Bipolaris sorokiniana conidia on wheat straw. The materials were observed at monthly intervals to assess the conidia viability during storage. After 4 months, the frequency of individual conidia already present on wheat straw at the time of sampling was reduced and appeared to be progressively replaced by the formation of round structures consist-ing of conidia aggregates. After 5 months, distinct, individual conidia were no longer detected, and only 'clumps of conidia' were observed. These dark black aggregates or 'clumps of conidia’measured 157-170$\mu\textrm{m}$ in diameter and were grouped into boat-shaped olivacious conidia showing thick wall and measuring 50-82$\times$20-30$\mu\textrm{m}$. The germination was unipolar and below 0.5%, suggesting the occurrence of dormancy, In contrast, individual conidium produced on wheat during the growing season were 96-130$\times$16-20$\mu\textrm{m}$, slightly curved, hyaline to light pale, and euseptate with a bipolar germination reaching 98-100%. Bipolaris sorokiniana conidia produced on PDA were 55-82$\times$20-27$\mu\textrm{m}$, tapered at both ends, dark brown to olivacious, distoseptate, showed up to 1% germination, and were predominantly unipolar. Results of the present study suggest that B. sorokiniana conidia belonged to two different physiological categories corresponding to the pathogen's infection phase and its survival, respectively. The infection phase is characterized by a high germination percentage as opposed to the survival phase harboring apparent dormancy.

Keywords

References

  1. Boosalis, M. G. 1962. Precocious sporulation and longevity ofconidia of Hetminthosporium sativum in soil. Phytopathotogy52:1172-1177
  2. Chin, S. H. F. 1953. A slide technique for the study of fungi andactinomycetes in soil with special reference to HeIminthospo-rium sativum. Can. J. Bot. 31:718-724 https://doi.org/10.1139/b53-053
  3. Chin, S. H. F., Lidingham, R. J. and Sallans, B. J. 1960. Popula-don and variability studies of HeIminthosporium sativum infield soils. Can. J. Bot. 31:718-724 https://doi.org/10.1139/b53-053
  4. Duczek, L. J. 1981. Number and viability of conidia ofCochtiobotus sativus in soil profiles in summer fallow fields inSaskatchewan. Can. J. Plant Pathol 3:12-14 https://doi.org/10.1080/07060668109501397
  5. Duczek, L. J., Jones-Flory, L. L., Reed, S. L., Bailey K. L. andLafond, G. P. 1996. Sporulation of Bipotaris sorokiniana onthe crowns of crop plants grown in Saskatchewan. Can. J.Plant Sci.76:861-S67 https://doi.org/10.4141/cjps96-146
  6. Duveiller, E. and Gilchhst, L. 1994. Production constraints due toBipolaris sorokiniana in wheat: Current situation and futureprospects, In: Wheat in Heat-Stressed Environments: Irri-gated, Dry Areas and Rice-Wheat Farming Systems, edited byD.A. Saunders & G.P. Hettel, PP. 343-352, CIMMYT, Mexico, D.F
  7. Duveiller, E. and Garcia Altamirano, I. 2000. Pathogenicity ofBipolaris sorokiniana isolates from wheat roots, leaves andgrains in Mexico. Plant Pathol. 49:235-242 https://doi.org/10.1046/j.1365-3059.2000.00443.x
  8. Dubin, H. J. and Duveiller, E. 2000. Helminthosporium leaf blights of wheat: integrated control and prospects for thefuture, Proceedings of the International Conference on Integrated Plant Disease Management for Sustainable Agriculture,New Delhi, India, 10-15 November, 1997, 1:575-579
  9. Duveiller, E., Garcia, I., Franco, J., Toledo, J., Crossa, J. andLopez, F. 1998. Evaluating spot blotch resistance of wheat:Improving disease assessment under controlled condition andin the field, In: Hetminthosporium Btights of Wheat: SpotBtotch and Tan Spot, edited by E. Duveiller, H.J. Dubin, J.Reeves and A. McNab, pp. 171-181, CIMMYT, Mexico, D.F.
  10. Lidingham, R. J. and Chinn, S. H. F. 1955. A flotation method forobtaining spore of Hetminthosporium sativum from soil. Can.J. Bot. 33:298-303 https://doi.org/10.1139/b55-025
  11. Luttrel, E. S. 1963. Taxonomic criteria in Hetminthosporium. Mycotoeia 55:643-674
  12. Mehta, Y. R. 1993. Spot blotch (BipoIaris sorokiniand). In: S.B.Mathur and B.M. Cunfer (eds.) Seed Borne Disease and SeedHeaIth Testine of Wheat. pp 105-112. Copenhagen, DenmarkInstitute of seed pathology for developing countries
  13. Misra, A. P. 1973. Hetminthosporium species occurring on cereals and other Gramineae. U.S.P.L. 480 Project No. A7-CR 133, Gmnt No. FG-IN-223. Tirhut College of Agriculture, Dholi,Muzaffarpur, Bihar, India. Catholic Press, Ranchi, Bihar,India. 289 p
  14. Nelson, R. R. and Kline, D. M. 1962. Interspecific variation in thepathogenicity in the genus Helminthosporium to graminaciousspecies. Phytopathology 52:1045-1049
  15. Reis, E. M. and Santos, H. P. 1987 The increased spomlation ofCochlioboIus sativus on aboveground tissue of small grainsand its relationship to the origin of inoculum in the soil. Fito-patologia Brasitaria 12:206-208
  16. Reis, E. M. 1991. Integrated disease management-The changingconcepts of controlling head blight and spot blotch. Proc. ofthe Wheat for Nontraditional Warm Areas Conference, Foz deIguazu, Brazil, 1990. ed. by D.A. Saunders & G. Hettel, pp.165-177, UNDP/CIMMYT, Mexico
  17. Reis, E. M., Casa, R. T. and M. S. Silva. 1995. Efeito do trata-mento de semontes de cevada no control e no deserivolvi-mento da mancha-em rede causada por DrechsIera teres.Fitopatol Bras 20:561-565
  18. Reis, E. M., Medeiros, C. A. and Casa, T. A. 1998. Control of leafblight of wheat by elimination of inoculum source, In: Hetm-inthosporium Blights of wheat: Spot Blotch and Tan Spot,edited by E. Duveiller, H.J. Dubin, J. Reeves and A. McNab,pp. 327-332, CIMMYT, Mexico, D.F
  19. Saah, E. E. 1998. Leaf blight diseases and associated soil bornefungal pathogens of wheat in south and south east Asia. In:Helminthosponum Blights of Wheat: Spot blotch and TanSpot, edited by E. Duveiller, H.J. Dubin, J. Reeves and A.McNab, pp. 37-51, CIMMYT, Mexico, D.F
  20. Simmonds, P. M., Sallans, B. J. and Ledingham, R. J, 1950. The occurrence of Helminthosporium sativum in relation to pri-mary infections in common root rot of wheat. Sci. Agric. 30:407-417

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