The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Cultural Characteristics of Xanthomonas axonopodis pv. citri Bacteriophages CP1from Korea

  • Myung, Inn-Shik (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Adminstration) ;
  • Nam, Ki-Woong (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Adminstration) ;
  • Cho, Yong-Sub (Division of Applied Biology and Chemistry, Seoul National University)
  • Published : 2002.12.01
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Abstract

Bacteriophage of Xanthomonas axonopodis pv. citri, a causal agent of citrus canker disease, was studied for its cultural characteristics. The relative efficiency of plat-ing (EOP) of 11 phages used to 13 strains off, axonopodis pv. citri tested ranged from 0.8 to 1, indicating that the phages are homogeneous. Homogeneity of the phages suggests that citrusphage belongs to a single group CPK as reported in a previous study. Typical one-step growth of a phage P5 selected from the citrusphages was observed. The EOP of the P5 was dependent upon the media, pH, and temperature. It was observed that multiplication of the phage cultured in Wakimotos potato semisynthetic media at $25^{\circ}C$ was more effective than that in other temperatures, regardless of the bacterial strains and media used. It was observed that pH 6.5 is optimal for multiplication of the phage. In comparison of the EOP among citrusphages $CP_1$, $CP_2$, and P5, multiplicative characteristic of phage P5 in the bacteria on time-course was similar with that of phage $CP_1$. Thus, it was concluded that citrusphage group CPK from Korea is $CP_1$ based on host specificity of the phage as described in a previous study, homogeneity, and its multiplication pattern.

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References

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