The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Rapid Identification of Potato Scab Causing Streptomyces spp. Using Pathogenicity Specific Primers

  • Shin, Pyung-Gyun (Plant Nutrition Division, National Institute of Agricultural Science and Technology, Rural Development Adminstration) ;
  • Kim, Jeom-Soon (Crop Division, National Alpine Agricultural Experiment Station, RDA) ;
  • Hahm, Young-Il (Crop Division, National Alpine Agricultural Experiment Station, RDA)
  • Published : 2002.12.01
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Abstract

The potato scab is caused by several species of Streptomyces. Among these species, only pathogenic strains were found to produce thaxtomin A characterized by necrotic bioassay and HPLC. In this study, identification of the pathogenic strains of Streptomyces was performed through the polymerase chain reaction (PCR) by using specific pathogenicity primer sets derived from the nec1 gene sequences of Streptomyces scabies. The expected PCR products were obtained approximately 580 bp and confirmed by sequencing. This PCR technique can be used effectively to identify the pathogenic Streptomyces species, that cause scab on potato tubers.

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References

  1. Babcock, M. J., Eckwall, E. C. and Schotte, J. L. 1993. Production and regulation of potato-inducing phytotoxins by Sterptomyces scabies. J. Gen. Microbiol. 139:1579-1586 https://doi.org/10.1099/00221287-139-7-1579
  2. Bukhalid, R. A., Chung, S. Y. and Loria, R. 1998. ned, a gene conferring a necrogenic phenotype, is conserved in plant pathogenic Streptomyces species and linked to a transposase pseudogene. Mol. Plant-Microbe Interact. 11:960-967 https://doi.org/10.1094/MPMI.1998.11.10.960
  3. Bukhalid, R. A. and Loria, R. 1997. Cloning and expression of a gene from Streptomyces scabies encoding a putative pathogenicity fector. J. Bacteriol. 179:7776-7783 https://doi.org/10.1128/jb.179.24.7776-7783.1997
  4. GreenShare. Potato disease: Scab. http://www.uri.edu/ce/factsheets/sheets/ potatoscab.html
  5. Kim, J. S., Park, D. H., Choi, Y. C., Lim, C. K., Hong, S. Y., Lee, S. D., Hahm, Y. I. and Cho, W. D. 1999. Potato scab caused by Streptomyces acidiscabies. Korean J. Plant Pathol. 14: 689-692
  6. King, R. R., Lawrence, C. H. and Clark, M. C. 1991. Correlation of phytotoxin production with pathogenicity of Sterptomyces scabies isolates from scab infected potato tubers. Am. Potato J. 68:675-680 https://doi.org/10.1007/BF02853743
  7. King, R. R., Lawrence, C. H., Clark, M. C. and Calhoun, L. A. 1989. Isolation and characterization of phytotoxins associated with Streptomyces scabies. J. Chem. Soc. 13:849-850
  8. Kuske, C. R., Banton, K. L., Adorada, D. L., Stark, P. C., Hill, K. K. and P. J. Jackson. 1998. Small-scale DNA sample preparation method for field PCR detection of microbial cells and spores in soil. Appl. Environ. Microbiol. 64:2463-2472
  9. Kutchma, A. J., Roberts, M. A. Knaebel, D. B. and Crawford, D. L. 1998. Small-scale isolation of genomic DNA from Streptomyces mycelia or spores. Biotechniques 24:452-457
  10. Leiner, R. H., Fry, B. A., Carling, D. E. and Loria, R. 1996. Probable involvement of thaxtomin A in pathogenicity of Streptomyces scabies on seedlings. Phytopathology 86:709-713 https://doi.org/10.1094/Phyto-86-709
  11. Loria, R., Bukhalid, R. A., Creath, R. A., Leiner, R. H., Olivier, M. and Steffens, J. C. 1995. Differential production of thaxtomins by pathogenic Streptomyces species in vitro. Phytopathotogy 85:537-541 https://doi.org/10.1094/Phyto-85-537
  12. Loria, R., Bukhalid, R. A., Fry, B. A. and King, R. R. 1997. Plant pathogenicity in the genus Streptomyces. Plant Dis. 81: 836-846 https://doi.org/10.1094/PDIS.1997.81.8.836

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