Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Occurrence, Type and Ultrastructure of Calcium Oxalate Crystals in Panax ginseng

인삼(Panax ginseng)에 존재하는 Calcium Oxalate 결정체의 분포, 유형 및 미세구조

  • Published : 2002.12.01
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Abstract

Crystalline calcium oxalate occur throughout near)y all plants species in five major forms; styloids, druses, raphids, prisms and sands. These crystals are known to be distributed in specific tissue such as cortex, xylem, phloem, cambium and epidermis. This research was undertaken to identify the occurrence, type, location and ultrastructure of druse crystals in Panax ginseng. In situ visualization, conventional light microscopy, histochemistry and scanning electron microscopy were applied for these purposes. Druse crystals in ginseng were identified as calcium oxalate by silver nitraterubeanic acid histochemistry. Calcium oxalate crystals are observed in nearly all plant organs such as leaf, petiole, peduncle, stem, rhizome, tap root and lateral root except fine root. Most frequent observation of crystals in the leaf and rhizomes were noticed. Three different types of calcium of oxalate druse crystals were identified by scanning electron microscopy.

본 연구는 인삼에 존재하는 calcium oxalate 결정체의 분포 위치, 형태 및 미세구조에 관하여 밝히고자 광학현미경, 조직화학적 방법 및 주사 전자현미경적 방법 등을 사용하여 연구 하였다. 인삼의 결정체는 calcium oxalate의 성분으로 구성되며, 결정체는 몸통뿌리, 지근, 뇌두, 줄기, 장엽병, 소엽병, 꽃대 등 대부분의 기관에서 관찰되었다 특히 지상부는 잎에서,지하부는 뇌두에서 가장 많은 결정체가 관찰되었다. 인삼에 존재하는 결정체는 silver nitrate-rubeanic acid를 사용한 조직화학적 반응을 실시한 결과 calcium oxalate치 성분으로 확인되었다. 또한 주사전자현미경으로 관찰한 결과 삼각뿔 형태의 결정체가 다수 모여서 형성된 유형, 프리즘 형태의 소결정이 모여서 형성된 유형 및 얇은 판상의 소결정이 모여서 형성된 유형 등 3가지 형태로 구분 할 수 있었다.

Keywords

References

  1. Gunning, B. E. S. and Steer. M. W. : The Study of Plant Structure, Edward Arnold Ltd., London, p. 37 (1975)
  2. Metcalfe, C. R. : Anatomy of Dicotyledons 2nd. ed., Pergamon Press, Oxford, p. 63 (1983)
  3. Franceschi, V. R. and Horner Jr, H. T. : Bot. Rev. 46, 361 (1980) https://doi.org/10.1007/BF02860532
  4. Frey-Wyssling, A. : Am. J. Bot. 68(1), 130 (1981) https://doi.org/10.2307/2443000
  5. Lersten, N. R. : Bot. Rev. 49, 233 (1983) https://doi.org/10.1007/BF02861088
  6. Nakata, P. A. and McConn, M. M. : Plant Physiol. 124(3), 1097 (2000) https://doi.org/10.1104/pp.124.3.1097
  7. Horner Jr, H. T. and Zindler-Frank, E. : Can. J. Bot. 60, 1021 (1981) https://doi.org/10.1139/b82-128
  8. Horner, H. T. and Weitmoyer, R. E. : J. Cell Sci II, 339 (1972)
  9. Pentecost, A. : Int. Rev. Cytol. 62, 1 (1980) https://doi.org/10.1016/S0074-7696(08)61897-5
  10. Mauseth, J. D. : Plant Anatomy, Benjamin Cummings, New York, p. 23 (1988)
  11. Kim, W. K., Park, H. D. and Jeong, B. K. : Plant Morphology and Anatomy, Academy Press, Seoul, p. 93 (1993)
  12. Fahn, A. : Plant Anatomy, Pergamon Press, Oxford, p. 23 (1990)
  13. Doaigey A. R. : Am. J. Bot. 78(12), 1608 (1991) https://doi.org/10.2307/2444842
  14. Arnott H. J. and Webb M. A. : Int. J. Plant Sci. 161(1), 133 (2000) https://doi.org/10.1086/314230
  15. Finley, D. S. : Revista de Biologia Tropical. 47, 27 (1999)
  16. Choi, Y. E., Harada. E., Wada. M., Tsuboi. H., Morita. M., Kusano. T., and Sano H. : Planta. 213, 45 (2001) https://doi.org/10.1007/s004250000487
  17. Leonard, R. T. and Hodges T. K. : The Plasmamembrane, HP Publishing Co. Inc., New York, p. 163 (1980)
  18. Pharmacopia Commission of PRC. : The Pharmacopia of China Vol. I, Chemical Industry Press, Beijing, p. 360 (1997)
  19. Yasue. T. : Acta Histochem Cytochem, 2, 83(1969) https://doi.org/10.1267/ahc.2.83
  20. Kim W. K. and Kim E. S. : Korean J. Electron Microscopy 10, 77 (1980)
  21. Li, X. and Franceschi, V. R. : Eu. J. Cell Bio. 51, 9 (1990)
  22. Kausch A. P. and Homa H. T. :Am. J. Bot. 70(5), 691 (1983) https://doi.org/10.2307/2443123
  23. Horner, H. T. and Wagner. Bruce L. : Am. J. Bot 79, 531 (1992) https://doi.org/10.2307/2444864
  24. Giaquinta, R. T. : Aust. J. Plant Physiol. 3, 619 (1979) https://doi.org/10.1071/PP9760619
  25. Wu C. C. and Kuo-Huang L. L. : Bot. Bull. Acad. Sci. 38, 97 (1997)
  26. Arnott H. J. and Webb M. A. : Int. J. Plant Sci. 161, 133 (2000) https://doi.org/10.1086/314230
  27. Bouropoulos N., Weiner S. and Addadi L. : Chemistry 7, 1881. (2001) https://doi.org/10.1002/1521-3765(20010504)7:9<1881::AID-CHEM1881>3.0.CO;2-I