Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular Cloning of the nahC Gene Encoding 1,2-Dihydroxynaphthalene Dioxygenase from Pseudomonas fluorescens

  • KIM, YEO-JUNG (Department of Biological Science, Sookmyung Women′s University) ;
  • NA-RI LEE (Department of Biological Science, Sookmyung Women′s University) ;
  • SOON-YOUNG CHOI (Division of Molecular Biology, Ewha Women′s University) ;
  • KYUNG-HEE MIN (Department of Biological Science, Sookmyung Women′s University)
  • Published : 2002.02.01
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Abstract

The complete nucleotide sequence of the nahC gene from Pseudomonas fluorescens, the structural gene for 1,2-dihydroxynaphthalene (1,2-DHN) dioxygenase, was determined. The 1,2-DHN dioxygenase is an extradiol ring-cleavage enzyme that cleaves the first ring of 1,2-dihydroxynaphthalene. The amino acid sequence of the dioxygenase deduced from the nucleotide sequence suggested that the holoenzyme consists of eight identical subunits with a molecular weight of approximately 34,200. The amino acid sequence of 1,2-DHN dioxygenase showed more than $90\%$ homology with those of the dioxygenases of other Pseudomonas strains. However, sequence similarity with those of the Sphingomonas species was less than $60\%$. The nahC gene of P. fluorescens was moderately expressed in E. coli NM522, as determined by enzymatic activity.

Keywords

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