Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Physico-Chemical and Rheological Properties of a Bioflocculant BF-56 from Bacillus sp. A56

  • Suh, Hyun-Hyo (Department of Environmental Engineering, Chinju National University) ;
  • Moon, Seong-Hoon (Biomolecular Process Engineering Laboratory) ;
  • Seo, Weon-Taek (Department of Food Processing, Chinju National University) ;
  • Kim, Kyung-Kab (Department of Environmental Engineering, Chinju National University) ;
  • Jeon, Gee-Ill (Department of Environmental Engineering, Chinju National University) ;
  • Park, Hyun-Geoun (Department of Environmental Engineering, Chinju National University) ;
  • Park, Yong-Il (Glycobiology Laboratory, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2002.04.01
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Abstract

Bacillus sp. A56 was studied, because of its high flocculating activity. The flocculating substance produced by this strain was purified by ethanol precipitation, cetylpyridinium chloride (CPC) precipitation, and gel permeation chromatography (GPC). The FT-IR spectrum of the purified bioflocculant, designated as BF-56, showed typical characteristics of polysaccharides. The non-sugar substituents, and sugar components of BF-56 containing glucose, fucose, glucuronic acid, and galactose in an approximate molar ratio of 2.76:1.10:1:0.12, suggested that it was a novel bioflocculant with an estimated molecular mass of over $7{\times}10^3$ kDa. Rheological analysis of BF-56 revealed that it was a pseudoplastic that had higher apparent viscosity rate at dilute concentrations than those of zooglan. The solution of bioflocculant BF-56 exhibited non-Newtonian characteristics and it was compatible to high concentrations of salts such as KCl, NaCl, $CaCl_2,\;or\;FeCl_3.$ The present results suggested strong possibility of bioflocculant BF-56 to be fully applicable to industries such as wastewater treatment.

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References

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