Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Anticaries Activity of Antimicrobial Material from Bacillus alkalophilshaggy JY-827

  • Chun, Ju-Yean (Department of Dental Hygiene, Wonkwang Health Science College) ;
  • Ryu, Il-Hwan (College of Life Science and Natural Resources, Wonkwang University) ;
  • Park, Jung-Sun (Department of Dental Hygiene, Wonkwang Health Science College) ;
  • Lee, Kap-Sang (College of Life Science and Natural Resources, Wonkwang University)
  • Published : 2002.02.01
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Abstract

The present study was performed to investigate the excellent microbial anticaries substance, aminoglycoside antibiotic, which is more effective than chlorhexidine for the treatment of dental caries. The aminoglycoside antibiotic against Streptococcus mutans JC-2 from a novel alkaliphilic Bacillus alkalophilshaggy JY-827 exhibited no significant difference at the treatment concentration of $2.5{\times}10^{-7}M$, however, it inhibited the activity of the Streptococcus mutans glucosyltransferase by 70.2% and 99.8% at the concentrations of $2.5{\times}10^{-7}$M\;and\;2.5{\times}10^{-6}M$, respectively. Lineweaver-Burk plot of the inhibitory aminoglycoside antibiotic showed competitive inhibition, with $K_i$ value of $6.4{\times}10^{-6}$ M. The aminoglycoside antibiotic did not show any cytotoxicity against human gingival cells. To evaluate the industrial applicability of the aminoglycoside antibiotic, a toothpaste containing this substance was prepared and tested on the extracted human teeth. The inhibitory rate of tooth calcification and calcium ion elution by the aminoglycoside antibiotic were 50% and 2.5 times, respectively. These results suggested that the aminoglycoside antibiotic from Bacillus alkalophilshaggy JY-827 is an effective agent against dental caries.

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References

  1. J. Clin. Peridentol. v.13 Chlorhexidin compared with other locally delivered antimicrobial, A short review Addy, M. https://doi.org/10.1111/j.1600-051X.1986.tb01434.x
  2. J. Biol. Chem. v.274 Inhibitory effect of a selfderived peptide on glucosyltransferase of Streptococcus mutans Akiko, E.;C. Takaomi;K. F. saido;T. Shigeo;I. Susumu;N. Tosiki;H. Nobuhiro https://doi.org/10.1074/jbc.274.22.15797
  3. Infect Immun. v.53 Cloning of Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis Aok, H.;T. Shiroza;M. Hayakawa;S. Satto;H. K. Kuramitsu
  4. Arch. Oral Biol. v.23 A comparison between chlorhexidin and some quaternary ammonium compound with regard to retention, salivary concentration, and plaque inhibiting effect in the human mouth after mouth rinse Bonesvoll, P.;P. Giermo https://doi.org/10.1016/0003-9969(78)90021-3
  5. Kor. J. Appl. Microbial Biotechnol. v.28 Isolation and identification of novel alkalophilic Bacillus alkalophilshaggy JY-827 with anticaries microbe Streptococcus mutans JC-2 Chun, J. Y.;I. H. Ryu;S. U. Lee;K. S. Lee
  6. Kor. J. Appl. Microbial Biotechnol. v.28 Production condition, purification and properties of anticaries microbial agent by Bacillus alkalopilshaggy JY-827 Chun, J. Y.;I. H. Ryu;S. U. Lee;K. S. Lee
  7. Arch. Oral. Biol. v.23 The effect of antibacterial compound on glucosyltransferase activity from Streptococcus mutans Ciardi, J. E.;W. H. Bowen;G. Rolla https://doi.org/10.1016/0003-9969(78)90023-7
  8. J. Exp. Pathol. v.5 On the bacterial factor in the aetiology of dental caries Clarke, J. K.
  9. Caries Res. v.3 The breakdown of the carbohydrate and protein matrix of dental plaque Critchley, P. https://doi.org/10.1159/000259599
  10. Arch. Oral Biol. v.44 Action of agents on glucosyl-transferase from Streptococcus mutans in solution and adsorbed to experimental pellicle David, W.;W. H. Bowen https://doi.org/10.1016/S0003-9969(98)00129-0
  11. Annu. Rev. Microbiol. v.29 Racterial adherence in oral microbial ecology Gibbons, R. T.;J. van Houte https://doi.org/10.1146/annurev.mi.29.100175.000315
  12. Phytothe Research v.3 Identification of antibacterial principle against Streptococcus mutans and inhibitory glucosyltransferase from the seed of Areca catechu L. Hada, S.;N. Kakiuchi;M. Hattori;T. Namba https://doi.org/10.1002/ptr.2650030406
  13. Infect. Immun. v.57 Isolation and characterization of the Streptococcus mutans gtf C gene, coding for primer dependent soluble glucan synthesis Hamada, N.;H. K. Kumamitsu
  14. Microbiol. Rev. v.44 Biology, immunology and cariogenicity of Streptococcus mutans Hamada, S.;H. D. Slade
  15. Infect. Immun. v.56 Isolation and characterization of the Streptococcus mutans gtf C gene, coding for synthesis of both soluble and insoluble glucan Hamada, N.;H. K. Kumamitsu
  16. J. Gen. Microbiol. v.116 Interaction of glucocyltransferase from Stereptococcus mutans with various glucans Hamada, S.;S. Toroii
  17. Infect. Immun. v.61 Nucleotide sequence analysis of the gtf-l gene from Streptococcus sobrinus OMZ176 Hanada, N.;Y. Isobe;Y. Aizawa;T. Katayama;S. Sato;M. Inoue
  18. Caries Res. v.6 Carbohydrate on pooled dental plaque Hotz, P.;B. Guggenheim;R. Schmid https://doi.org/10.1159/000259783
  19. J. Korean Acad. Periodontology v.23 The effect of honoliol and magnolol on the antimicrobial, bacterial collagenase activity, cytotoxicity and cytokine production Jang, B. S.;C. P. Chung;S. H. Son;K. H. Bae
  20. Antibiotics Jung, D. H.
  21. Chunbuk Graduated thesis Effect of propolis against caries activity with Streptococcus mutans Kim, D. B.
  22. Wonkwang Graduated thesis Effect of tannin and vitamin B6 on the growth and attachment to the test tube wall of Streptococcus mutans 10449 Kim, S. S.
  23. Arch. Oral Biol. v.45 In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens Koo, H.;B. P. F. A. Gomes;P. L. Rosalen;G. M. B. Ambrosano;Y. K. Park;J. A. Cury https://doi.org/10.1016/S0003-9969(99)00117-X
  24. J. Clin. Periodont. v.13 The antimicrobial and clinical effects of a single subgingival irrigation of chlorhexidine in advanced periodontal lesions Lander, P. Z.;G. M. Newcomb;G. J. Seymour;R. N. Dowell https://doi.org/10.1111/j.1600-051X.1986.tb01417.x
  25. J. Lipid Res. v.37 Neomycin inhibits secretion of apolophoprotein by increasing retention on the hephatocyte cell sulface Lanford, R. E.;L. Estlack;A. L. White
  26. KP 91-2095 Toothpaste Composition Lucky Company
  27. Amer. Soc. Pharm. Exp. Therap v.227 Impairment of membrane phosphoinositide metabolism by aminoglycoside antibiotics, streptomycin, amicacin, kanamycin, dibekacin, gentamycin and neomycin Marche, P.;S. Koutouzov;A. Girard
  28. Infect. Immun. v.23 Musaka, T.;M. Tsunezuka;Y. Takehara;S. Nunome
  29. Appl. Environ. Microbiol. v.59 Inhibitory effect of oolong tea polyphenol on glucosyltransferase of Streptococcus mutans Nakahara, K.;S. Kawabata, H. Ono;K. Ogura;T. Ooshima;S. Hamada
  30. Caries Research v.27 Oolong tea polyphenols inhibit experimental dental caries in SPF tat infected with Streptococcus mutans Ooshima, T.;T. Minami;W. Aono;A. Izumitami;S. Sobue;T. Fujiwara;S. Kawabata;S. Hamada https://doi.org/10.1159/000261529
  31. J. Korean Acad. Dental Health v.19 Effect of Phellodendri cortex L. on the activity of glucosyltransferase and human Gingival cell, growth and membrane permeability of Streptococcus mutans JC-2 Park, C. S.;Y. S. Shin
  32. J. Korean Acad. Periodontology v.21 Effect of Phellodendri cortex L. on the activity of glucosyltransferase and human gingival cell, growth and membrane permeability of Streptococcus mutans Park, C. S.;Y. S. Shin;K. S. Lee;I. H. Kang;S. S. Kim
  33. J. Biol. Chem. v.269 N-butyldeoxynojirimycin is a novel inhibitor of glycolipid biosynthesis Platt, F. M.;G. R. Neises;R. A. Dwek;T. D. Butters
  34. J. Clin. Periodontol. v.14 Antimicrobial properties of two aliphatic amines and chlohexidin in vitro and in saliva Salem, A. M.;D. Adams;H. N. Newman;L. W. Rawle https://doi.org/10.1111/j.1600-051X.1987.tb01512.x
  35. Molecular Basis of Oral Microbial Adhesion Virulence of mutans defective in glucosyltransferase, dextran, mediated aggregation or dextranase activity Tanzer, J. M.;M. L. Freedman;R. J. Fitzgerald;Mergenhagen, S. E.(ed.);Rosan, B.(ed.)
  36. Caries Res. v.30 Characterization of glucosyltransferase of human saliva adsorbed on to hydroxyapatite surfaces Vacca-Smith, A. M.;A. R. Venkitaraman;K. M. Schilling;W. H. Bowen https://doi.org/10.1159/000262342
  37. Infect. Immun. v.61 Role of the Streptococcus mutans fif genes in caries induction in the specific-pathogen-free rat model Yamashita, Y.;W. H. Bowen;R. A. Burne;H. K. Kuramitsu