Determination of Hydrogen Peroxide Concentration by Portable Near-Infrared (NIR) System

근적외분광분석법을 이용한 과산화수소의 농도 측정

  • 임현량 (동덕여자대학교 약학대학) ;
  • 우영아 (동덕여자대학교 약학대학) ;
  • 장수현 (동덕여자대학교 약학대학) ;
  • 김경미 (동덕여자대학교 약학대학) ;
  • 김효진 (동덕여자대학교 약학대학)
  • Published : 2002.10.01


This experiment was carried out to determine non-destructively the hydrogen peroxide concentration of 3% antiseptic hydrogen peroxide solutions by portable near-infrared (NIR) system. Hydrogen peroxide standards were prepared ranging from 0 to 25.6 w/w% and the NIR spectra of hydrogen peroxide standard solutions were collected by using a quartz cell in 1 mm pathlength. We found the variation of absorbance band due to OH vibration of hydrogen peroxide depending on the concentration around 1400 nm in the second derivatives spectra. Partial least square regression (PLSR) and multilinear regression (MLR) were explored to develop a calibration model over the spectral range 1100-1720 nm. The model using PLSR was better than that using MLR. The calibration showed good results with a standard error of prediction (SEP) of 0.16%. In order to validate the developed calibration model, routine analyses were performed using commercial antiseptic hydrogen peroxide solutions. The hydrogen peroxide values from the NIR calibration model were compared with the values from a redox titration method. The NIR routine analyses results showed good correlation with those of the redox titration method. This study showed that the rapid and non-destructive determination of hydrogen peroxide in the antiseptic solution was successfully performed by portable NIR system without very harmful solvents.


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