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The Pharmaceutical Society of Korea (대한약학회)
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Suppression of Phorbol Ester-Induced NF-kB Activation by Capsaicin in Cultured Human Promyelocytic Leukemia Cells

  • Han, Seong-Su (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Keum, Young-Sam (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Chun, Kyung-Soo (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Surh, Young-Joon (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University)
  • Published : 2002.08.01
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Abstract

Capsaicin, a major pungent constituent of red pepper (Capsicum annuum L.) possesses a vast variety of pharmacologic and physiologic activities. Despite its irritant properties, the compound exerts anti-inflammatory and anti-nociceptive effects. Previous studies from this laboratory revealed that capsaicin, when topically applied onto dorsal skin of female ICR mice, strongly attenuated activation of NF-kB and AP-1 induced by the typical tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), which may account for its anti-tumor promoting activity in mouse skin. In the present work, we have found that capsaicin suppresses TPA-stimulated activation of NF-kB through inhibition of $IkB{\alpha}$ degradation and blockade of subsequent nuclear translocation of p65 in human pro myelocytic leukemia HL-60 cells. Methylation of the phenolic hydroxyl group of capsaicin abolished its inhibitory effect on NF-kB DNA binding. Likewise, TPA-induced activation of AP-1 was mitigated by capsaicin treatment.

Keywords

References

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