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The Pharmaceutical Society of Korea (대한약학회)
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Simultaneous Determination of the Novel Neuroprotective Agent KR-31378 and its Metabolite KR-31612 Using High Performance Liquid Chromatography with Tandem Mass Spectrometry in Human Plasma

  • Kim, John (Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Department of Clinical Research, LG Life Sciences, Ltd) ;
  • Ji, Hye-Young (Medicinal Resources Research Center and Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, Wonkwang University) ;
  • Yoo, Sung-Eun (Korea Research Institute of Chemical Technology) ;
  • Kim, Sun-Ok (AgroPharma Research Institute, Dongbu Hannong Chemical Company) ;
  • Lee, Dong-Ha (Department of Clinical Research, LG Life Sciences, Ltd.) ;
  • Lim, Hong (Department of Clinical Research, LG Life Sciences, Ltd.) ;
  • Lee, Hye-Suk (Medicinal Resources Research Center and Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, Wonkwang University)
  • Published : 2002.10.01
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Abstract

An LC/MS/MS method for the simultaneous determination of a neuroprotective agent for ischemia-reperfusion damage, KR-31378 and its N-acetyl metabolite KR-31612 in human plasma was developed. KR-31378, KR-31612 and the internal standard. KR-31543 were extracted from human plasma by liquid-liquid extraction. A reverse-phase HPLC separation was performed on Luna phenylhexyl column with the mixture of acetonitrile-5 mM ammonium formate (55:45, v/v) as mobile phase. The detection of analytes was performed using an electrospray ionization tandem mass spectrometry in the multiple reaction monitoring mode. The lower limits of quantification for KR-31378 and KR-31612 were 2.0 ng/ml. The method showed a satisfactory sensitivity, precision, accuracy, recovery and selectivity.

Keywords

References

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