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The Pharmaceutical Society of Korea (대한약학회)
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Immunomodulating and Anticoagulant Activity of Glycosaminoglycans Derived from Porcine Testis

  • Yoo, Yung-Choon (Department of Microbiology, Konyang University) ;
  • Kim, Yeong-Shik (Natural Products Research Institute, Seoul National University) ;
  • Song, Kyung-Sik (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University) ;
  • Moon, Eun-Ho (General Education/Mathematics Myongji University) ;
  • Lee, Kyung-Bok (Department of Biochemistry, College of Medicine, Konyang University)
  • Published : 2002.10.01
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Abstract

Glycosaminoglycans (GAGs) were isolated from the porcine testis, and their immuno-modulating and anticoagulant activity was investigated. From anion exchange chromatography (Dowex Macropolous Resin) used for further isolation of porcine testis GAGs (PT-GAGs), two fractions (PT-GAG-1.5 and PT-GAG-16) eluted by different salt concentration were obtained. In immunomodulating activity test, PT-GAG-1.5, but not PT-GAG-16, significantly enhanced the growth of murine peritoneal macrophages. In addition, treatment with PT-GAG-1.5 induced the production of cytokines, interleukin-1$\beta$ (IL-1$\beta$), interferon-${\gamma}$ (IFN-${\gamma}$) and tumor necrosis factor-$\alpha$ (TNF-$\alpha$), from murine microphages. Unexpectedly, both of PT-GAGs had no effect on the growth of murine splenocytes. The anticoagulant activity of PT-GAG-1.5 and PT-GAG-16 was examined by activated partial thromboplastin time (aPTT) assay and thrombin time (TT) assay. Both of PT-kGAGs significantly increased the clotting times of aPTT and TT in a dose-dependent manner. The anticoagulant activity of PT-GAG-16 was found to be higher than that of PT-GAG-1.5. These results suggest that PT-GAGs possess biological activities such as immunomodulating activity and anticoagulant activity.

Keywords

References

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