The Korean Journal of Nuclear Medicine (대한핵의학회지)

The Korean Society of Nuclear Medicine (대한핵의학회)
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Decreased glucose uptake by hyperglycemia is regulated by different mechanisms in human cancer cells and monocytes

사람 암세포와 단핵세포에서 고포도당 농도에 의한 FDG 섭취 저하의 서로 다른 기전

  • Kim, Chae-Kyun (Department of Nuclear Medicine, Cancer Research Institute Seoul National University College of Medicine) ;
  • Chung, June-Key (Department of Nuclear Medicine, Cancer Research Institute Seoul National University College of Medicine) ;
  • Lee, Yong-Jin (Department of Nuclear Medicine, Cancer Research Institute Seoul National University College of Medicine) ;
  • Hong, Mee-Kyoung (Department of Nuclear Medicine, Cancer Research Institute Seoul National University College of Medicine) ;
  • Jeong, Jae-Min (Department of Nuclear Medicine, Cancer Research Institute Seoul National University College of Medicine) ;
  • Lee, Dong-Soo (Department of Nuclear Medicine, Cancer Research Institute Seoul National University College of Medicine) ;
  • Lee, Myung-Chul (Department of Nuclear Medicine, Cancer Research Institute Seoul National University College of Medicine)
  • 김채균 (서울대학교 의과대학 핵의학교실, 암연구소) ;
  • 정준기 (서울대학교 의과대학 핵의학교실, 암연구소) ;
  • 이용진 (서울대학교 의과대학 핵의학교실, 암연구소) ;
  • 홍미경 (서울대학교 의과대학 핵의학교실, 암연구소) ;
  • 정재민 (서울대학교 의과대학 핵의학교실, 암연구소) ;
  • 이동수 (서울대학교 의과대학 핵의학교실, 암연구소) ;
  • 이명철 (서울대학교 의과대학 핵의학교실, 암연구소)
  • Published : 2002.04.30
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Abstract

To clarify the difference in glucose uptake between human cancer cells and monocytes, we studied $[^{18}F]$ fluorodeoxyglucose (FDG) uptake in three human colon cancer cell lines (SNU-C2A, SNU-C4, SNU-C5), one human lung cancer cell line (NCI-H522), and human peripheral blood monocytes. The FDG uptake of both cancer cells and monocytes was increased in glucose-free medium, but decreased in the medium containing 16.7 mM glucose (hyperglycemic). The level of Glut1 mRNA decreased in human colon cancer cells and NCI-H522 under hyperglycemic condition. Glut1 protein expression was also decreased in the four human cancer cell lines under hyperglycemic condition, whereas it was consistently undetectable in monocytes. SNU-C2A, SNU-C4 and NCI-H522 showed a similar level of hexokinase activity (7.5 - 10.8 mU/mg), while SNU-C5 and monocytes showed lower range of hexokinase activity (4.3 - 6.5 mU/mg). These data suggest that glucose uptake is regulated by different mechanisms in human cancer cells and monocytes.

목적: FDG PET은 악성종양의 진단에 유용하게 쓰이고 있으나, 염증에도 섭취되어 진단에 어려움이 있다. 염증에서 F-18-FDG 섭취는 단핵세포에서 포도당대사가 항진되어 나타난다. 이 연구에서는 사람의 암세포와 단핵세포간에 포도당대사에 차이가 있는지 알아보고자 하였다. 대상 및 방법: 사람의 대장암 세포주(SNU-C2A, SNU-C4, SNU-C5)와 폐암 세포주(NCI-H522), 단핵세포를 포도당 농도가 다른 배지에서 각각 배양시키고, FDG 섭취와 포도당운반체 1(Glut1)의 발현, hexokinase 활성도의 변화를 비교 분석하였다. 결과: 포도당이 없는 배지에서는 암세포와 단핵세포 모두에서 FDG 섭취가 증가되나 포도당 고농도(16.7 mM)에서는 섭취가 감소하였다. 이 고농도에서 Glut1 mRNA의 발현은 대장암 세포주, 폐암 세포주에서 감소하였다. 고농도의 포도당 배지에서 Glut1 단백질의 발현도 4종류의 암세포에서 모두 감소하였으나, 단핵세포에서는 변화가 없었다. SNU-C2A, SNU-C4, NCI-H522 세포에서 hexokinase의 활성도는 비슷하였고, 단핵세포와 SNU-C5에서는 약간 증가하였다. 결론: 포도당 섭취에 있어서 사람의 암 세포주와 단핵세포는 서로 다른 기전을 보이고 있다. 대장암 세포는 포도당 농도에 의한 포도당 섭취 변화가 Glut1에 의하여 조절되나, 단핵세포는 다른 기전을 가지고 있다.

Keywords

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