토끼모델에서 저강도 맥동초음파가 골절치유에 미치는 영향

The Effect of Low-Intensity Pulsed Ultrasound on Fracture Healing in the Rabbit Model

  • 김종만 (서남대학교 보건학부 물리치료학과) ;
  • 이충휘 (연세대학교 보건과학대학 재활학과 및 보건과학연구소) ;
  • 조상현 (연세대학교 보건과학대학 재활학과 및 보건과학연구소) ;
  • 박정미 (연세대학교 원주의과대학 재활의학교실) ;
  • 권혁철 (대구대학교 재활과학대학 재활과학과) ;
  • 황태선 (포천중문 의과대학교 해부학교실)
  • Kim, Jong-Man (Dept. of Physical Therapy, Division of Health, Seonam University) ;
  • Yi, Chung-Hwi (Dept. of Rehabilitation Therapy, College of Health Science, Yonsei University Institute of Health Science, Yonsei University) ;
  • Cho, Sang-Hyun (Dept. of Rehabilitation Therapy, College of Health Science, Yonsei University Institute of Health Science, Yonsei University) ;
  • Park, Jung-Mi (Dept. of Rehabilitation Medicine, Wonju Christian Hospital, Wonju College of Medicine, Yonsei University) ;
  • Kwon, Hyuk-Cheol (Dept. of Rehabilitation Technology, College of Rehabilitation Science, Taegu University) ;
  • Hwang, Tae-Sun (Dept. of Anatomy, College of Medicine, Pocheon CHA University)
  • 발행 : 2002.02.19

초록

The purpose of this research was to determine the effects on the healing of fibular fractures in rabbits of low-intensity pulsed ultrasound (50 $mW/cm^2$ and 500 $mW/cm^2$) applied for periods of 4, 14 and 24 days following fibular osteotomy. Thirty-six male Japanese white rabbits were randomly divided into three groups of twelve for three treatment protocols: (1) ultrasound treatment at intensities of 50 $mW/cm^2$ and 500 $mW/cm^2$ until the 4th day following fibular osteotomy, (2) ultrasound treatment at intensities of 50 $mW/cm^2$ and 500 $mW/cm^2$ until the 14th day following fibular osteotomy, and (3) ultrasound treatment at intensities of 50 $mW/cm^2$ and 500 $mW/cm^2$ until the 24th day following fibular osteotomy. The low-intensity pulsed ultrasound was applied to only one fibula of each rabbit (these served as the experimental group). The other fibula of each rabbit served as the control group. The selection of which fibula was to be treated was made randomly. The animals were sacrificed on the 4th, 14th and 24th day after the start of ultrasound treatments. Percent of trabecular bone area and fibular radiography were carried out to compare the degree of fibular bone healing. A microscope was also used to determine any histologic changes. For statistical differences in radiological changes due to length of treatment period (4, 14 and 24 days respectively), the Wilcoxon signed-ranks test was used to compare the experimental and control groups. For statistical differences in fracture healing due to differences in ultrasound intensity, radiological studies were compared using the Mann-Whitney Test. And, to compute percentage differences in areas of trabecular bone, Two-way analysis of variance (ultrasound intensity x each group) was used. Experiment results were as follows: 1. In animals sacrificed on the 4th day, no difference was found in the radiological studies of the fibulae in the experimental and control groups (p>.05). However, experimental groups showed more rapid bone repair than control group. 2. Both radiographic and percent of trabecular bone area studies showed significant differences in rabbits sacrificed after 14 days. Fracture healing was significantly increased in the experimental group (p<.05) 3. In the animals sacrificed on the 24th day, histologic study showed rapid bone repair but fibular radiologic studies did not show statistical differences between the two groups (p>.05). 4. On the 14th day, bone union on radiograph was significantly more rapid in the treatment group with pulsed ultrasound of 50 $mW/cm^2$ than the group with 500 $mW/cm^2$ (p<.05). Histologic studies showed that both the 14 and 24 days groups had more rapid bone repair in animals treated with 50 $mW/cm^2$ ultrasound intensity than those treated with 500 $mW/cm^2$ intensity. In conclusion, it has been shown that the low-intensity pulsed ultrasound has a positive effect on bone fracture healing in the early stage and the range of pulse ultrasound from 50 $mW/cm^2$ to 500 $mW/cm^2$ is effective for fracture healing. Further study is needed to investigate the influence of pulsed ultrasound on delayed union and non-union in bone fractures and also for the clinical use of low-intensity pulsed ultrasound for bone healing in humans.

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