Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Cell Cycle Arrest by Sabaek-san is Associated with induction of Cdk Inhibitor p21 in Human Lung Cancer A549 Cells

사백산에 의한 인체 폐암세포의 G1기 성장억제기전에 관한 연구

  • Kang Byong Ryeung (Department of Internal Medicine, Dongeui University and Research Institute of Oriental Medicine) ;
  • Oh Chang Sun (Department of Internal Medicine, Dongeui University and Research Institute of Oriental Medicine) ;
  • Lee Jae Hun (Department of Biochemistry, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine) ;
  • Choi Yung Hyun (Department of Biochemistry, College of Oriental Medicine, Dongeui University and Research Institute of Oriental Medicine) ;
  • Park Dong Il (Department of Internal Medicine, Dongeui University and Research Institute of Oriental Medicine)
  • 강병령 (동의대학교 한의과대학 폐계내과학교실) ;
  • 오창선 (동의대학교 한의과대학 폐계내과학교실) ;
  • 이재훈 (동의대학교 한의과대학 생화학교실 및 한의학연구소) ;
  • 최영현 (동의대학교 한의과대학 생화학교실 및 한의학연구소) ;
  • 박동일 (동의대학교 한의과대학 폐계내과학교실)
  • Published : 2002.12.01
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Abstract

We investigated the effects of Sabaek-san (SBS) water extract on the cell proliferation of human lung carcinoma A549 cells. SBS treatment resulted in the inhibition of cell proliferation in a concentration-dependent manner. This anti-proliferative effect of A549 cells by SBS treatment was associated with morphological changes such as membrane shrinking and cell rounding up. DNA flow cytometric histograms showed that population of G1 phase of the cell cycle was increased by SBS treatment in a concentration-dependent manner. SBS treatment induced a marked accumulation of tumor suppressor p53 and a concomitant induction of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP, which appears to be transcriptionally upregulated and is p53 dependent. In addition, SBS treatment resulted in down-regulation of cyclooxygenase-2 (COX-2) as determined by RT-PCR analysis. The present results indicated that SBS-induced inhibition of lung cancer cell proliferation is associated with the blockage of G1/S progression the induction of apoptosis.

Keywords

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