Protective Effect of Cornu Cervi Parvum Extract on Toxicity Induced by 2,3,7,8-Tetrachlorodibenzo-p-dioxin in Rat

다이옥신-유도 독성에 대한 녹용 추출물의 방어효과

  • Hwang Seock Yeon (Department of Clinical Pathology, Chungbuk National University Hospital) ;
  • Yang Jin Bae (College of Oriental Medicine, Taejon University) ;
  • Chang Cheoul Soo (Department of Clinical Pathology, Kimcheon Health Siences College) ;
  • Lee Young Chan (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Lee Hyung Chul (Department of Pathology, College of Oriental Medicine, Wonkwang University)
  • 황석연 (충북대학교 임상병리과) ;
  • 양진배 (대전대학교 한의과대학) ;
  • 장철수 (김천대학 임상병리과) ;
  • 이영찬 (원광대학교 한의과대학 병리학교실) ;
  • 이형철 (원광대학교 한의과대학 병리학교실)
  • Published : 2002.08.01


The toxicity and bioaccumulation of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and polychlorinated biphenyls (PCBs) continues to be a focus of research in human and various species. The main human exposure is via the dietary route. This study was carried out to investigate the protective effect of Cornu Cervi Parvum extract on clinical parameters and hepatotoxicity in Sprague-Dawley rat (SD rat) accutely exposured to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Male SD rats received single intraperitoneal (ip) injection of TCDD (40 ㎍/kg), and administered 10 or 20 mg/kg/day of the ethanol extract oral injection for 4 weeks from 1 week before TCDD treatment. The gain in body weight was less in group treated with TCDD than in CON group, while that of C/H+ TCDD group (Cornu Cervi Parvum extract 20 mg/kg/day) increased. The decrease in spleen and testis weight caused by TCDD was prevented by Cornu Cervi Parvum extract 20 mg/kg/day. The fluctuation in BUN content, WBC and platelet count by TCDD intoxication were significantly attenuated by the ethanol extract treatment (20 mg/kg/day for 4 weeks). Treatments of rats with the extract (10 or 20 mg/kg/day) were significantly reduced AST and ALT levels compared with TCDD-treated group. Moderate swelling of hepatocytes, hyperchromatism, acidophilic cytoplasm and cytoplasmic vacuolation were observed in TCDD-treated animals (TCDD group). The administration of EtOH extract 10 or 20 mg/kg along with TCDD significantly alleviated the liver histopathological alteration induced by TCDD. These results suggest that Cornu Cervi Parvum extract can be useful as a protective agent against TCDD, an endocrine disruptor.



  1. Envion. Health Perspect. v.101 Our stolen Future. Putton, New York, endocrine-disruption chemicals in wildlife and humans. Colborm, T.;Dumanoski, D.;Myers, J.P.
  2. Environ. Health Perspect. v.105 The challenge posed by endocrine-disrupting chemicals. Ashby, J.;Houthoff, E.;Kennedy, S.J.;Stevens, J.;Bars, R.;Jekat, F.W.;Campbell, P.;Miller, J.V.;Carpanini, F.M.;Randall, G.L.P.
  3. Eur. J. Cancer. Prev. v.1 Inadvertant exposure to xenoestrogens. Olea, N.;Pazos, P.;Exposito, J.
  4. Crit. Rev. Toxicol. v.24 Polychlorinated biphenyls (PCBs): environmental impact, biochemical and toxic responses, and implications for risk assessment. Safe, S.H.
  5. Ecotoxicol. Environ. Saf. v.45 Evaluation of endocrine modulators: implication for human health. Juberg, D.R.
  6. Ann. Rev. Pharmacol. Toxicol. v.22 2,3,7,8-tetrachlorodibenzo- p-dioxin and related halogenated aromatic hydrocarbons: examination of the mechanism of toxicity. Poland, A.; Knuston J. C.
  7. Toxicol. Appl. Pharmacol. v.35 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD): results of a 13-week oral toxicity study in rats. Kociba, R.J.;Keeler, P.A.;Park, C.N.;Gehring, P.J.
  8. Toxicol. Appl. Pharmacol. v.145 In utero and lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin : effects on development of the male and female reproductive system of the mouse. Theobald, H.M.;Peterson, R.E.
  9. Ann. Neurol. v.32 The oxidant stress hypothesis in Parkinson's disease: evidence supporting it. Fahn, S.;Cohen, G.
  10. Lancet v.344 Free radicals, antioxidants and human disease: curiosity, cause, or consequence? Halliwell, B.
  11. 약제학회지 v.6 녹각이 cholesterol 투여 가토의 간 조직 및 각 장기에 미치는 영향 용재익
  12. 한국생화학회지 v.15 녹용추출액의 조혈작용에 관한 연구 김길원;박시원
  13. 경희대한의대 논문집 v.10 녹각 수지침이 CCl4 중독 휜쥐 손상 간에 미치는 영향 박종현;고형균;김창환
  14. 대한한의학회지 v.2 Cholesterol 투여 가토의 혈청 중 cholesterol에 미치는 가토의 영향 용재익
  15. 경희대학교 한의과대학 논문집 v.3 녹각이 백서 간장 조직에 미치는 영향에 관한 조직화학적 연구. 이학인
  16. 한국식품영양과학회지 v.28 2,3,7,8-Tetrachlorodibenzo -p-dioxin 투여로 급성독성을 유도한 웅성 기니픽에 있어 임상화학지수에 미치는 홍삼의 효과. 황석연;김시관;김신희;곽이성;정영진
  17. Cell Bio. Toxicol. v.7 Long-term carcinigenesis studies of 2,3,7,8-tetrachlorodibenzo- p-dioxin and hexachloro-dibenzo-p-dioxin. Huff, J.E.;Salmon, A.G.;Hopper, N.K.;Zeise, L.
  18. J. Clin. Invest. v.55 Effect of renal funtion in the rat. Kirachi, R.;Fleischer, K.;Kamisaka;Arias, I.M.
  19. Chem. Biol. Interact. v.10 Induction and Suppression of hepatic and extra-hepatic microsoomal foreign compound metabolizing enzyme system by 2,3,7,8-TCDD. Hook, G.E.;Haseman, R.J.K.;Lucier, G.W.
  20. Food Cosmet. Toxicol. v.11 Toxic effect 2,3,7,8-tetrachlorodibenzo-p-dioxin. Greig, J.B.;Jones, G.;Butler, W.H.;Barnes, J.H.
  21. Bull. Environ. Contam. v.7 Effect of tetrachlorodibenzo-p-dioxin on growth rate and synthesis of lipid and proteins in rats. Cunningham, H.M.;Williams, D.T.
  22. Cancer Res. v.52 Dose-response relationships for chronic exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin in rat tumor promotion model: Quantification and immunolocalization of CYP1A1 and CYP1A2 in the liver. Tritscher, A.M.;Goldstein, J.A.;Portier, C.J.;McCoy, M.;Clark, G.C.;Lucier, G.W.
  23. Naturwiss v.59 Organism as targets of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in toxication. Buu-Hoi, N.P.;Harris;Moore, J.A.;Vos, J.G.
  24. Arch. Environ. Health. v.32 Epidemiology and pathology of a 2,3,7,8- tetrachlorodibenzo-p-dioxin poisoning episode. Kimbrough, R.D.;Carter, C.D.;Liddle, J.A.;Cline, R.E.;Phillips, P.E.
  25. 和韓醫學會誌 v.5 Identification of the inhibitor for monoamine oxidase B in the extract from deet antler (Rokujo). Wang, B.X.;Zhao, X.H.;Yang, X.W.;Kaneko, S.;Hattori, M.;Namba, T.;Namura, Y.