Effects of Ginseng Radix Against Osteoblasts Damaged by Endocrine Disrupting Chemicals

환경호르몬에 의해 손상된 골모세포에 대한 인삼의 영향

  • Hong Gi Youn (Department of Obstetric and Gynecology, School of Medicine, Wonkwang University) ;
  • Park Seung Taeck (Institution for Medical Science, Wonkwang University) ;
  • Jeon Byung Hun (College of Oriental Medicine, Wonkwang University) ;
  • Seo Eun A (Medicinal Resources Research Center, Wonkwang University)
  • 홍기연 (원광대학교 의과대학) ;
  • 박승택 (원광의과학연구소) ;
  • 전병훈 (원광대학교 한의과대학) ;
  • 서은아 (원광대학교 의학자원연구센타)
  • Published : 2002.04.01

Abstract

In order to evaluate the effect of Ginseng Radix(GR) against endocrine disrupting chemicals(EDC), cultured mouse osteoblasts were preincubated with various concentrations of GR extract before the exposure of Bisphenol A for 12 hours. Cytotoxic effect of Bisphenol A was measured by the XTT assay. In addition, the protective effect of GR over Bisphenol A-induced cytotoxicity on osteoblasts was assessed by the DNA and protein synthesis in these cultures. The results were as follows : Osteoblastic cell viability was decreased in dose and time dependent manner after exposed to various concentrations of Bisphenol A. Midcytotoxicity value(MCV50) of Bisphenol A was determined at 6μM Bisphenol A after osteoblasts were grown for 12 hours in the media containing various concentrations of Bisphenol A. Amount of DNA synthesis was increased in dose-dependent manner after cultured osteblasts were pretreated with GR for 2hrs before exposure to Bisphenol A for 12 hours. Amount of protein synthesis was increased in dose-dependent manner after cultured osteoblasts were pretreated with GR for 2 hours before exposure of Bisphenol A for 12 hours. From these results, it is suggested that Bisphenol A was highly toxic by the decrease of the cell viability, and GR is effective in the prevention of Bisphenol A-induced cytotoxicity by the increase of DNA and protein syntheses in cultured mouse osteoblasts.

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