Applied Microscopy

  • 제32권2호
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  • Pages.149-156
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  • 2002
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
한국현미경학회 (Korean Society of Microscopy)
권호 표지

활성탄이 납 중독된 흰쥐의 간장에 미치는 조직학적 연구

Histological Study on the Activated Carbon in the Rat Liver with Toxicated by Lead

  • 정민주 (조선대학교 자연과학대학 생물학과) ;
  • 노영복 (조선대학교 자연과학대학 생물학과)
  • Chung, Min-Ju (Dept. of Biology, College of Natural Science, Chosun University) ;
  • Roh, Young-Bok (Dept. of Biology, College of Natural Science, Chosun University)
  • 발행 : 2002.06.01
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⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 흰쥐의 간장에서 납 독성에 대한 활성탄 효과를 연구하는데 그 목적이 있다. 실험동물은 $7{\sim}8$주된 150 g 내외의 Sparague-Dewley계 웅성 Rat를 사용하였다. lead acetate (500 ppm)를 1주 2회 복강 투여하였고 활성탄(40 mg/kg)을 1일/1회씩 경구투여하여 4주, 8주째에 간장을 적출해서 투과전자현미경으로 관찰한 바 다음과 같은 결과를 얻었다. 납 단독투여 4주군은 과립형질내세망의 수조가 팽대되고, 리보솜의 탈락이 관찰되었다. 핵은 약간 함입되어 불규칙하였으며 핵막은 팽대되어 내외막과 핵공이 불분명하게 나타났다. 납-활성탄 투여 4주군은 과립형질내세망은 리보솜이 부착된 정상적인형태로 관찰되었다. 다수의 리소좀이 관찰되었다. 핵은 둥근 형태로 관찰되었다. 납 단독투여 8주군은 과립형질내세망의 수조가 더욱 심하게 팽대되어 공포화를 나타냈다. 사립체는 더욱 팽창되서 사립체능이 절단, 손실되어 낮은 전자밀도를 나타냈다. 핵은 약간 함입되어 불규칙하고 핵막이 팽대되었다. 납-활성탄 투여 8주군은 일부 과립형질내세망의 수조가 팽대되었으나 리보솜이 부착된 정상적인 형태로 핵 주위에 전형적인 층판구조를 형성하였다. 사립체는 약간 팽창되었고, 핵은 정상으로 보였다.

For investigation of the activated carbon in the rat liver toxicated by lead. Aniamls used $7{\sim}8$ weeks to Sparague-Dewley rat (150 g). The lead acetate (500 ppm) were injected and activated carbon (40 mg/kg) were treated orally for four and eight weeks, respectivelly, and observed by the electron microscope. The group with only lead for 4 weeks, The mitochondria and rough endoplasmic reticulum (rER) were extended and ribosomes dropped from the rER. The group with lead-activated carbon for 4 weeks, The number of lysosomes increased. The shapes of nucleus and rough endoplasmic reticulum observed almost similar with nomally. The group with only lead for 8 weeks, The mitochondria and rough endoplasmic reticulum (rER) were more extended. The group with lead-activated carbon for 8 weeks, the nucleus and rough endoplasmic reticulum was observed nomally.

키워드

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