Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Perturbation of host responses by Porphyromonas gingivalis biofilm

Porphyromonas gingivalis 바이오필름에 의한 숙주 면역반응의 교란

  • Jeon, Woo-Seok (Department of Periodontology, College of Dentistry, Pusan National University) ;
  • Kim, Sung-Jo (Department of Periodontology, College of Dentistry, Pusan National University) ;
  • Choi, Jeom-Il (Department of Periodontology, College of Dentistry, Pusan National University)
  • 전우석 (부산대학교 치과대학 치주과학교실) ;
  • 김성조 (부산대학교 치과대학 치주과학교실) ;
  • 최점일 (부산대학교 치과대학 치주과학교실)
  • Published : 2002.12.31
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Abstract

The present study was performed to evaluate how cellular and humoral immune responses were perturbed by immunization of mixed periodontal bacterial biofilms. Each group of mice was immunizared with 1) Poqhyromonas gingivalis (P. gingivaliis) grown as a planktonic culture, 2) Fusobacterium nucleatum (F. nucleatum), 3) P. gingivalis grown as a biofilm, or 4) mixed P. gingivalis plus F. nucleatum grown as a biofilm culture, respectively. Immune mouse sera were collected from each mouse. Spleens were harvested to isolate T cells and consequently stimulated with antigen presenting cells and P. gingivalis whole cell antigen to establish P. gingivalis-specific T cell lines. There were no significant differences in the mean anti- gingivalis IgG antibody titers among mouse groups. Immunization of mice with pure P. gingivalis biofilm or mixed P gingivalis plus F. nucleatum biofilm resulted in significant reduction o f antibody avidity and opsonophagocytois function. INF-$\gamma$production by P. gingivalis-specific T cell lines was also substantially recluced in mouse groups immunized with the biofilm. It was concluded that P. gingivalis biofilm perturbs the cellular and humoral immune responses in periodontal disease.

Keywords

References

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