Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Bacteriological Characteristics of Unidentified Vibrio sp., Hemolysin Producer Isolated from Brackish Water -2. Bacteriological Characteristics of Vibrio sp. E10 Similar to Vibrio mimicus-

기수에서 분리된 용혈독소를 생산하는 미분류 Vibrio sp.의 세균학적 특징 -2. Vibrio mimicus와 유사한 Vibrio sp. E10의 세균학적 특성-

  • Kim Young Man (Department of Food Science and Nutrition, Dong-eui University) ;
  • Yu Hong Sik (Watervision Co.,) ;
  • Oh Hee Kyung (Department of Food Science and Nutrition, Dong-eui University)
  • Published : 2002.11.01
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Abstract

A hemolysin producing bacterial strain which belong to Vibrio species was isolated from the Kum River estuary. In the process of identification, the strain did not show characteristics of known Vibrio species; thus, the strain was designated as Vibrio sp, E10 (V. kunsan) tentatively and further identification study was carried out by comparing its bacteriological characteristics. Morphologically Vibrio sp, E10 was comma shaped rod with a polar flagellium. Clear hemolysis zones were observed with the strain against human and sheep blood agar. Hemollytic toxicity was confirmed by strong vascular Permeability and fatal toxicity against mouse was also observed. Therefore the strain was a pathogenic vibrio. Growth conditions for Vibrio sp. E10 were ranged salinity of 0$\~$$4.5\%$, pH of 6.2$\~$9.2, temperature of 14$\~$42$^{\circ}C$, respectively, 16S rDNA partial sequence of Vibrio sp, E10 showed $99\%$ homology with dozens of V. cholerae species including V, cholerae El Tor N16961 and V, snmisnfus ATCC 33653T. This strain belonged to Proteobacteria; gamma subdivision; Vibrionacea: Vibrio. But, among knorn Vibrio species no identical styains were found when using automatic bacteria identification system ($MicroLog^{TM}$system, release 4.0, Biolog Inc., USA) which evaluated the ability of metabolizing 95 kinds of carbon and nitrogen sources. Vibrio sp, E10 showed 18 and 11 different responses as compared to V. mimicus and V, cholerae, respectively.

금강 하구에서 분리한 용혈독소를 생산하는 V. mimicus와 유사하지만 분류되지 않은 기수성 Vibrio속으로 추정되는 균주 Vibrio sp. E10 (V. kunsan)의 세균학적 특징은 다음과 같다. 기본적인 생화학적 시험과 전자현미경 상의 형태에서는 V. mimicus와 유사하였지만 TCBS 배지 상의 colony 형태는 V. vulnifirus와 유사하였으므로 V. mimicus와는 차이를 보였다. Human과 sheep blood agar에서의 뚜렷한 용혈환과 rat에 피부혈관투과항진 작용으로 용혈독성이 있었으며, mouse에 대한 치사독성이 있었으므로 병원성이 확인되었다. 생육 가능한 환경 조건은 염도 0$\~$$4.5\%$, pH 6.2$\~$9.2, 온도 $14\~42^{\circ}C$이 었다. Vibrio sp. E10의 16S rDNA 염기서열은 10여종의 V. cholerae와 1종의 Vibrio mimicus의 염기서열과 $99\%$의 상동성을 보여 유사 종으로 판명되었다. 그러나 세균 자동동정기를 이용한 동정 결과, genus는 Vibrio 속으로 동정되었으나 species는 일치하는 균종이 없었다. 알려진 Vi-brio속 중에서 유사성이 높았던 V. mimicus와 비교해 볼 때 18종류의 항목이, V. cholerae와는 11종류의 항목이 다른 반응을 나타내어 미분류 균종으로 확인되었다.

Keywords

References

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