Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Genetic Identification on Natural Population of Triploid Crucian Carp, Carassius auraus in Korea

자연산 3배체 붕어 (Carassius auratus) 클론 집단에 대한 유전학적 동정

  • Kim Eung Oh (Chung Pyong Inland Fisheries Institute, NFRD) ;
  • LEE Jong Yoon (Chung Pyong Inland Fisheries Institute, NFRD) ;
  • Nam Yoon Kwon (Department of Aquaculture, Pukyong National University) ;
  • Noh Jae Koo (Department of Aquaculture, Pukyong National University) ;
  • Lee Sang Yun (Department of Aquaculture, Pukyong National University) ;
  • Kim Dong Soo (Department of Aquaculture, Pukyong National University)
  • Published : 2002.11.01
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Abstract

Natural clonal stock of triploid crucian carp, Carassius aurahs was identified and its cytogenetic, molecular genetic and morphological traits were studied. Cytogenetic analysis of the clonal crucian carp revealed that they were natural triploidy, evidenced by 1.5-fold increases of cell size, DNA content, and chromosome number. Multi-locus DNA fingerprinting using $(GATA)_4$ probe showed that they had an identical fingerprint profile, indicating the clonal propagation of the population. External morphology and morphometric characteristics of triploid individuals were much uniform compared to those of diploids. Natural triploid crucian carp was proven to be all-female in this study.

국내에 서식하는 붕어 (Carassius auratus) 3배체 집단을 동정하고 이들을 대상으로 분자생물학적, 세포유전학적 및 형태학적 특징을 분석하였다. 본 자연산 붕어 3배체 집단은 전형적인 3배체의 세포 유전학적 특성들, 즉 이배체에 비해 1.5배의 세포 및 핵의 크기, 3n=150의 염색체수, 그리고 2배체 (3.6pg/cell)에 비해 1.5배 증가된 DNA 함량 (5.4 pg/cell)을 나타내었다. 또한 본 3배 체붕어 집단은 DNA fingerprinting 분석에 의해 유전적인 구성이 동일한 클론 집단인 것으로 판명되었고 클론 3배체 붕어들은 2배체와 비교시 매우 일양한 형태학적 특성을 나타내었으며 모두 암컷인 전암컷 집단이었다. 본 연구의 분석에 사용된 함안 집단의 3배체 붕어 집단 이외에도 논산, 주문진, 속초 및 삼례 붕어 집단을 대상으로 3배체 출현 빈도를 조사한 결과, 모든 지역에서 $80\%$ 이상의 높은 3배체 빈도를 보였다.

Keywords

References

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