Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Preparation Condition of Chitooligosaccharide by Cellulase using Response Surface Methodology

반응표면 분석법을 이용하여 Cellulase에 의한 키토산올리고당의 제조 조건 설정

  • Joo Dong Sik (Dept. of Tourism and Foodservice Industry, Donghae University) ;
  • Lee Jung Suck (East coastal Marine Bioresources Research Center, Kangnung National University) ;
  • Kim Ok Seon (East coastal Marine Bioresources Research Center, Kangnung National University) ;
  • Cho Soon Yeoung (East coastal Marine Bioresources Research Center, Kangnung National University)
  • 주동식 (동해대학교 관광외식산업학과) ;
  • 이정석 (강릉대학교 동해안해양생물자원연구센터) ;
  • 김옥선 (강릉대학교 동해안해양생물자원연구센터) ;
  • 조순영 (강릉대학교 동해안해양생물자원연구센터)
  • Published : 2002.11.01
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Abstract

Optimal conditions for preparing of chitooligosaccharides from chitosan with cellulase was researched by response surface methodo-logy, Penicillium funiculosum derived cellulase was most effective for chitooligosaccharides production as the point of hydrolyzing activity and commercial utility. The result which measures the change of degrading ratio at time course, 10 hr reaction showed a exponential increase and after that time degrading ratio was not changed. The optimal conditions determined by response surface methodology with central composite design of total 26 species were $0.5\%$ of chitosan, 143 U enzyme, 49$^{\circ}C$ of reaction temperature, 13.2 hr of reaction time and pH 3.8. Major chitooligosaccharides produced from chitiosan on optimal conditions were dimer and trimer.

Cellulase를 이용하여 키토산 올리고당을 제조하기 위한 최적조건을 반웅표면 분석법을 이용하여 설정하였다 키토산 분해능과 산업적 활용의 측면에서 유리한 Pen. funiculosum 유래의 cello-lase를 예비 실험을 통해 결정하였다. 이 효소의 적정 반응 조건에서 경시적 키토산 분해율을 측정한 결과, 반응 10시간까지는 대수적 증가를 보였으나, 10시간 이후로는 완만한 분해율을 나타내었다. 중심합성계획에 의해 총 26개의 실험을 행한뒤 반응표면분석법으로 설정한 키토산 분해 최적 조건은 기질 농도 $0.5\%$에 대해 효소 농도 143(U), 반응 온도 $49^{\circ}$, 반응 시간 13.2hr및 pH는 3.8이었다. 본 연구에서 설정된 최적의 조건에서 얻어진 키토산 분해물은 dimer와 trimer가 주된 올리고당이었고, 소량의 tetramer와 hexamer 그리고 monomer 등의 총 8종 정도의 올리고당이 검출되었다.

Keywords

References

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