홍화자약침액(紅花子藥鍼液)이 수은(水銀)에 의한 간세포(肝細胞) 손상(損傷)에 미치는 영향(影響)

The Effect of Carthami Semen Aquacupuncture on HgCl2-Induced Liver Cell Injury

  • 박재영 (동의대학교 한의과대학 침구경혈학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구경혈학교실 및 한의학연구소) ;
  • 장경전 (동의대학교 한의과대학 침구경혈학교실 및 한의학연구소) ;
  • 송춘호 (동의대학교 한의과대학 침구경혈학교실 및 한의학연구소) ;
  • 안창범 (동의대학교 한의과대학 침구경혈학교실 및 한의학연구소)
  • Park, Jae-young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Youn, Hyoun-min (Department of Acupuncture & Moxibustion, College of Oriental Medicine and Research Institute of Oriental Medicine, Dong-Eui University) ;
  • Jang, Kyung-jeon (Department of Acupuncture & Moxibustion, College of Oriental Medicine and Research Institute of Oriental Medicine, Dong-Eui University) ;
  • Song, Choon-ho (Department of Acupuncture & Moxibustion, College of Oriental Medicine and Research Institute of Oriental Medicine, Dong-Eui University) ;
  • Abn, Chang-beobm (Department of Acupuncture & Moxibustion, College of Oriental Medicine and Research Institute of Oriental Medicine, Dong-Eui University)
  • 투고 : 2002.07.13
  • 심사 : 2002.09.14
  • 발행 : 2002.10.20

초록

Objective : This study was undertaken to examine whether Carthami Semen aquacupuncture (CSA) exerts protective effect against Hg-induced cell injury in rabbit liver. Methods : The cell injury was evaluated by ALT activity and lipid peroxidation was estimated by measuring malondialdehyde (MDA). Results : Hg caused an increase of ALT activity and lipid peroxidation in a dose-dependent-manner over concentrations of 0.1-1 mM, which were prevented by addition of 0.005% CSA. The protective effect of CSA was dose-dependent in concentration range of 0.001 to 0.01%. The increase of ALT activity and lipid peroxidation induced by 0.5 mM Hg were almost completely decreased by addition of 0.01% CSA. When the liver tissues were exposed to 0.5 mM Hg, GSH content was decreased, which was significantly restored by 0.01% CSA. 0.5 mM Hg caused decrease in the amount of total and nonprotein sulfhydryl groups, and 0.01% CSA prevented Hg-induced reduction of nonprotein sulfhydryl group but not protein sulfhydryl group. Conclusions : These results suggest that CSA exerts protective effect against Hg-induced cell injury by antioxidant action resulting from enhancement of nonprotein sulfhydryl group content including GSH in liver.

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