Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Effect of Scutellaria baicalensis Georgi Extract on Oxidant-Induced Inhibition of Organic Cation in Rabbit Renal Cortical Slices

황금약침액(黃芩藥鍼液)이 가토(家兎)의 신피질절편(腎皮質切片)에서 Oxidant로 유발된 유기양이온의 이동장애에 미치는 영향(影響)

  • Son, In-suk (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Cho, Tae-sung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Kwon, Hae-yon (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Jo, Mi-hyeong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Youn, Hyoun-min (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Jang, Kyung-jeon (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Song, Choon-ho (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Abn, Chang-beohm (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University)
  • 손인석 (동의대학교 한의과대학 침구경혈학교실) ;
  • 조태성 (동의대학교 한의과대학 침구경혈학교실) ;
  • 권혜연 (동의대학교 한의과대학 침구경혈학교실) ;
  • 조미형 (동의대학교 한의과대학 침구경혈학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구경혈학교실) ;
  • 장경전 (동의대학교 한의과대학 침구경혈학교실) ;
  • 송춘호 (동의대학교 한의과대학 침구경혈학교실) ;
  • 안창범 (동의대학교 한의과대학 침구경혈학교실)
  • Received : 2002.03.07
  • Accepted : 2002.03.20
  • Published : 2002.04.20
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Abstract

Objective : This study was undertaken to determine whether Scutellaria baicalensis Georgi extract (SbG) exerts the protective effect against oxidant-induced alterations in organic cation transport in the renal proximal tubule. Methods : Organic cation transport was estimated by examining alterations in tetraethylammonium (TEA) uptake in rabbit renal cortical slices. The slices were treated with 0.2 mM tBHP for 60 min at $37^{\circ}C$. tBl-IP caused an inhibition in TEA uptake by renal cortical slices. Such an effect was accompanied by depressed Na+-K+-ATPase activity and ATP depletion. Result : SbG prevented tBHP-induced inhibition of TEA uptake in a dose-dependent manner at the concentration ranges of 0.05-0.1%. SbG also prevented H2O2-induced reduction in TEA uptake. tBHP-induced inhibition of Na+-K+-ATPase activity and ATP depletion were significantly prevented by 0.05% SbG. Oxidants increased LDH release, which was blocked by SbG. Oxidants caused a significant increase in lipid peroxidation and its effect was prevented by SbG. Conclusion : These results suggest that SbG prevents oxidant-induced alterations in organic cation transport in rabbit renal cortical slices. Such protective effects of SbG may be attributed to inhibition of peroxidation of membrane lipid.

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