The Journal of Korean Academy of Prosthodontics (대한치과보철학회지)

The Korean Academy of Prosthodonitics (대한치과보철학회)
권호 표지

CYTOTOXICITY OF DENTURE BASE RESINS

의치상 레진의 세포독성에 관한 연구

  • Kim Seong-Kyun (Department of Prosthodontics, Graduate School, Seoul National University) ;
  • Chang Ik-Tae (Department of Prosthodontics, Graduate School, Seoul National University) ;
  • Heo Seong-Joo (Department of Prosthodontics, Graduate School, Seoul National University) ;
  • Keak Jai-Young (Department of Prosthodontics, Graduate School, Seoul National University)
  • 김성균 (서울대학교 치과대학 치과보철학교실) ;
  • 장익태 (서울대학교 치과대학 치과보철학교실) ;
  • 허성주 (서울대학교 치과대학 치과보철학교실) ;
  • 곽재영 (서울대학교 치과대학 치과보철학교실)
  • Published : 2002.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to investigate the cytotoxicity and mutagenicity of denture base resins. According to manufacturer's instructions, resin specimens were made. Group 1 : heat-polymerizing acrylic resin (Luciton $199^{(R)}$) Group 2 : heat-polymerizing acrylic resin containing polyhedraloligosilsesquioxane(POSS resin) Group 3 : auto-polymerizing acrylic resin (Repair $Acrylic^{(R)}$) Group 4 : direct relining auto-polymerizing acrylic resin (Tokuso $Rebase^{(R)}$). Fresh specimens 24 hrs. and 72 hrs. soaked specimens in distil)ed water were made. Responses with metabolic assay and mutagenesis assay to eluates from resin specimens were measured. Cultures with medium alone provided controls. Cytotoxicity was assessed with agar overlay test. The results were as follows; 1. Group 4 showed higher cytotoxicity than Group 1, Group 2 and Group 3 in fresh, 24-an4 72-hour immersion caries (p<.05). Group 3 showed higher cytotoxicity than Group 2 in fresh cases and showed higher cytotoxicity than Group 1 and Group 2 in 24-and 72-hour immersion cases (p<.05) . Group 1 and Group 2 showed no significant difference. 2. All acrylic denture base resins skewed significant increase of cell activity as immersion time increased (p<.05). 3. Auto-polymerizing acrylic denture base resins skewed higher cytotoxicity than heat-polymerizing acrylic denture base resins (p<.05). 4. All acrylic denture base resins showed lower mutagenicity than controls (p<.05).

Keywords

References

  1. Lee DK, Lee YK, Yoo SK, Kim KJ, Lee HS, Lee KH, Han DS. Development of in vitro evaluation model in dental biocompatibi1ity by non-radioactive materials. J Oral Biology 1995;19:129-136
  2. Hensten-Pettersen K, Jacobsen N. Toxic effects of dental materials. Int Dent J 1991;41:265-273
  3. Olsen I. Denture stomatitis. Acta Odonto1 Scand 1975;33:41-46 https://doi.org/10.3109/00016357509004625
  4. Wictorin L, Anneroth G, Fritiof L. Denture stomatitis. A clinical, e1ectronmicroscopic, microradiographic and lightmicroscopic study. Acta Odonto1 Scand 1975;33:299-311 https://doi.org/10.3109/00016357509004635
  5. Kaaber S, Thulin H, Nielsen E. Skin sensitivity to denture base materials in the burning mouth syndrom. Contact Dermatitis 1979;5:90-96 https://doi.org/10.1111/j.1600-0536.1979.tb04805.x
  6. McCabe JF, Basker RM. Tissue sensitivity to acrylic resin. Br Dent J 1976; 140:347-350 https://doi.org/10.1038/sj.bdj.4803760
  7. Guinta JL, Grauer I, Zablotsky N. Allegic contact stomatitis caused by acrylic resin. J Prosthet Dent 1979;42:188-190 https://doi.org/10.1016/0022-3913(79)90173-2
  8. Bohnenkamp DM. Traumatic stomatitis following an intraoral denture reline: a clinical report, J Prosthet Dent 1996;76:113-114 https://doi.org/10.1016/S0022-3913(96)90292-9
  9. Baker S, Brooks SC, Walker DM. The release of residual monomeric methyl metacrylate from acrylic appliances in the human mouth: An assay for monomer in saliva. J Dent Res 1988;67:1295-1299 https://doi.org/10.1177/00220345880670101001
  10. Lefebvre CA, Kooerndchild KL, Schuster GS. Cytotoxicity of eluates from light-polymerized denture base resins. J Prosthet Dent 1994;72:644-650 https://doi.org/10.1016/0022-3913(94)90298-4
  11. Bates JF, Stafford GD, Huggett R, Handley RW. Current status of pour type denture base resins. J Dent 1977;5:177-189 https://doi.org/10.1016/0300-5712(77)90001-X
  12. Sheridan PJ, Koda S, Ewolden NO, Lefebvre CA, Lavin MT, Cytotoxicity of denture base resins. Int J Prosthodont 1997;10:73-77
  13. Cimpan MR, Cressey LI, Halstensen A, Lie SA, Gjertsen BT, Matre R, Patterns of cell death induced by eluates from denture base acrylic resins in U-937 human monoblastoid cells. Eur J Oral Sci 2000;108:59-69
  14. Vallittu PK, Ekstrand K. In vitro cytotoxicity of fibre-polymethyl metacrylate composite used in dentures. J Oral Rehabil 1999;26:666-671 https://doi.org/10.1046/j.1365-2842.1999.00431.x
  15. Hensten-Pettersen A, Wictorin L. The cytotoxic effect of denture base polymers. Acta Odontol Scand 1981;39:101-106 https://doi.org/10.3109/00016358109162267
  16. Yang HJ, Jang BS, Heo SJ, Han DH, Shim JS, Chang MW. The effects of denture cleansers and disinfectants on the color. surface hardness. surface roughness of denture base resins. J Korean Acad Prosthodont 2001;39: 105-112
  17. International Standards Organization. ISO Technical Report 7405-1997. Dentistry-Preclinical evaluation of biocompatibility of medical devices used in dentistry-Test methods for dental materials. Geneva, Switzerland, 1997
  18. Flecher AM, Purnaveja A, Amin WM, Ritchie GM, Moradians S, Dodd AW. The level of residual monomer in self-curing denture-base materials. J Dent Res 1983; 62:118-120 https://doi.org/10.1177/00220345830620020601
  19. Schuster GS, Marr JC, Knoernschild KL. The effect of ph on the cytotoxicity of eluates from denture base resins. Int J Prosthodont 1995;8:122-128
  20. Koda T, Tsuchiya H, Yamauchi M, Ohtani S, Takagi N, Kawano J. Leachability of denture base acrylic resins in artificial saliva. Dent Mater 1990;6:13-16 https://doi.org/10.1016/0109-5641(90)90037-F
  21. Vallittu PK, Miettinen V, Alakuijala P. Residual monomer content and its release into water from denture base materials. Dent Mater 1995;11:338-342 https://doi.org/10.1016/0109-5641(95)80031-X
  22. Sadamori S. Kotani H. Hamada T. The usage period of dentures and their residual monomer contents. J Prosthet Dent 1992;68:374-376 https://doi.org/10.1016/0022-3913(92)90349-F
  23. Ruyter IE. The release of formaldehyde from denture base polymers. Acta Odontol Scand 1980;38:17-27 https://doi.org/10.3109/00016358008997715
  24. Oysaed H, Ruyter IE, Sjovikskleven IJ. Release of formaldehyde in dental composites. J Dent Res 1988;67:1289-1294 https://doi.org/10.1177/00220345880670100901
  25. Katsuno K, Manabe A, Itoh K, Hisamitsu H, Wakumoto S, Nakayama S, Yoshida T. A delayed hypersensitivity reaction to dentin primer in the guinea-pig. J Dent 1995: 23: 295-300
  26. Kedjarune U, Charoenworaluk N, Koontongkaew S. Release of methyl metacrylate from heat-cured and autopolymerized resins: Cytotoxicity testing related to residual monomer. Aust Dent J 1999;44:25-30 https://doi.org/10.1111/j.1834-7819.1999.tb00532.x
  27. Taira M, Nakao H, Matsumoto T, Takahashi J. Cytotoxic effect of methyl metacrylate on 4 cultured fibroblasts. Int J Prosthodont 2000;13:311-315
  28. Lygre H, Solheim E, Gjerdet NR. Leaching from denture base materials in vitro. Acta Odontol Scand 1995;53:75-80 https://doi.org/10.3109/00016359509005950
  29. Tsuchiya H, Yamada K, Akagiri M, Tajima K, Miyazaki T, Takagi N, Itoh U, Sato M. Effect of an ultraviolet light-activated coating material on reduction of the leaching of methyl metacrylate and formaldehyde from denture acrylic resins. Dent Mater 1993;12:253-258 https://doi.org/10.4012/dmj.12.253
  30. Smith DC. Recent development and prospects in dental polymers. J Prosthet Dent 1962;12:1066-1078 https://doi.org/10.1016/0022-3913(62)90162-2
  31. Liu Y, Loftenius A, Elghazali G, TroyeBlomberg M, Ma S, Ekstrand J. Different regulation of in vitro cytokine production by human blood cells in response to methyl metacrylate. J Toxico Environ Health 1999;56:165-182 https://doi.org/10.1080/009841099158132
  32. Tyas MJ. Quantitative enzyme cytochemistry in the in vitro biocompatibility testing of dental materials. Int Endod J 1988;21:106-112 https://doi.org/10.1111/j.1365-2591.1988.tb00963.x
  33. Browne RM. The in vitro assessment of the cytotoxicity of dental materials-dose it have a role?. Int Endod J 1988;21:50-58 https://doi.org/10.1111/j.1365-2591.1988.tb00955.x
  34. Rosenbluth SA, Weddington GR, Guess WL, Autian J. Tissue culture method for screening toxicity of plastic materials to be used in medical practice. J Pharm Sci 1965;54:156-159 https://doi.org/10.1002/jps.2600540139
  35. Caughman WF, Caughman GB, Dominy WT, Schuster GS. Glass ionomer and composite resin cements: Effects on oral cells. J Prosthet Dent 1990;63:513-521 https://doi.org/10.1016/0022-3913(90)90067-M
  36. Kasugai S, Hasegawa N, Ogura H. Application of the MTT colorimetric assay to measure cytotoxic effects of phenolic compounds on established rat dental pulp cells. J Dent Res 1991;70:127-130 https://doi.org/10.1177/00220345910700020601