Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

NPY Distribution in Diencephalon of Developing Chick

발생중인 병아리의 사이뇌에서 NPY 양성반응세포의 분포

  • Lee, Mee-Young (College of Veterinary Medicine, Chungnam National University) ;
  • Park, Il-Kwon (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Kyoung-Youl (College of Veterinary Medicine, Chungnam National University) ;
  • Kwon, Hyo-Jung (College of Veterinary Medicine, Chungnam National University) ;
  • Song, Chi-Won (College of Veterinary Medicine, Chungnam National University) ;
  • Park, Mee-Sun (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Kang-Iee (College of Oriental Medicine, Taejon University) ;
  • Ha, Kwon-Soo (Korea Basic Science Institute) ;
  • Kim, Won-Sik (College of Medicine, Chungnam National University) ;
  • Kim, Moo-Kang (College of Veterinary Medicine, Chungnam National University)
  • Accepted : 2002.02.21
  • Published : 2002.03.25
Download PDF
⟨ Previous Next ⟩

Abstract

This study deals with the distribution and morphological study of the neurons with the neuropeptide Y (NPY) in developing chick brain. The developing brains of Korean native chicks at embryonic days 8 (E8), E10, E12, E14, E16, E18, and E20 were used. The chicks were perfused with 4% paraformaldehyde through the left ventricle and aorta. The brains were removed and transferred into 30% sucrose, and then cut in a cryostat into $60{\mu}m$ in thickness. The sections were immunostained with free-floating and avidin-biotin complex (ABC) methods. The numerous neurons with NPY were first observed in nucleus rotundus of diencephalon at E8. In particular, neurons in nucleus rotundus had the well-developed processes. At E12, the neurons with NPY were distributed widely in diencephalon; nucleus septalis lateralis, medialis, nucleus magnocelluaris preopticus dorsalis, medialis, ventralis, nucleus preopticus medialis, nucleus dorsolateralis anterior thalami pars magnocellularis, and nucleus paraventricularis magnocellularis (paraventricular nucleus) except nucleus rotundus. From E12 to E20, positive neurons were oval-shaped, changed gradually into the spherical- and multipolar-shaped. The shapes of processes were also omnidirectional and the number of those were less than in telencephalon. As the chicks developed, the morphology of neurons with NPY showed the tendency to increase in their sizes and numbers.

Keywords

References

  1. Allen YS, Andrian TE, Allen JM, Tatemoto, K, Crow TJ, Bloom SR and Polk JM. Neuropeptide Y distribution in the rat brain. Science 221, 877-879. 1983
  2. Kuenzel WJ and McMurtry JM. Neuropeptide Y: brain localization and central effects on plasma insulin levels in chicks. Physiol. Behav. 44, 669-678. 1988
  3. Lundberg JM, Hokfelt T, Anggard A, Terenius L, Elde R, Markey K, Goldstein M and Kimmel J (1982). Organizationl principle in the peripheral sympathetic nervous system : subdivision by coexisting peptides(somatostain-, avian pancreatic polypeptide-, and vasoactive intestinal polypeptide-like immunoreactive materials). Proc. Natl. Acad. Sci. USA 79, 303-1307
  4. Clark JJ, Kalra PS, Crawley WR, Kalra SP. Neuropeptide Y and human pancreatic polypeptide stimulate feeding behavior in rats. Endocrinology 115 : 427-431, 1984
  5. B. Glen Stanley, Sarah F. Leibowitz. Neuropeptide Y injected in the paraventricular hypothalamus : A powerful stimulant of feeding behavior. Neurobiology. 82 : 3940-3943. 1985
  6. Gray TS, Morley JE. Neuropeptide Y : Anatomical distribution and possible function in mammalian nervous system. Life Sci 38 : 389-401, 1986
  7. Stanley BG, Daniel DR., Chin AS and Leibowits SF Paraventricular nucleus injections of peptide YY and neuropeptide Y preferentially enhance carbohydrateingestion. Peptides 6:1205-1211, 1985
  8. Yvan Dumont, Alain Fournier. Expression and Characterization of the Neuropeptide Y Y5 Receptor subtype in the Rat Brain. The Journal of Neuroscience, August 1, 18(15) : 5565-5574, 1998
  9. Albers HE, Ferris CF. Neuropeptide Y : Role in lightdark cycle entrainment of hamster circadian rhythms. Neurosci Lett 50 : 163-168, 1984
  10. Kalra SP, Crowley WR. Norepinephrine-like effects of neuropeptide Y on LH release in the rat. Life Sci 35 : 1173-1176, 1984
  11. Lundberg JM, Terenius L, Hökfelt T, Marthing CR, Tatemoto K, Mutt V et al. Neuropeptide Y(NPY)-like immunoreactivity in periperal noradrenergic neuronsand effects of NPY on sympathetic function. Acta physiol Scand 116 : 477-480, 1982
  12. Allen JM, Adrian K, Tatemoto K, Polak JM, Hughes J, Bloom SR. Two novel related peptides, neuropeptide Y(NPY) and peptide YY(PYY), inhibit the contraction of the electrically stimulated mouse vas deferens. Neuropeptides 3 : 71-77, 1982
  13. Allen LG, Kalra PS, Crowley WR, Kalra SP. Comparison of the effect of neuropeptide Y and adrenergic transmitters on LH release and food intake in male rats. Life Sci 37 : 617-623, 1985
  14. Mcdonald JK, Lumpkin MD, Samson WK. Mclann SM. Neuropeptide Y affects secretion of lutenizing hormone and growth hormone in ovariectomized rats.Proc Natl Acad Sci 82 : 561-564, 1985
  15. Crowley WR, Hassid A, Kalra SP. Neuropeptide Y enhances the release of lutenizing hormone induced by lutenizing hormone-releasing hormone. Endocrinology 120 : 941-945, 1987
  16. Fuxe K, Agnati LF, Harfstrand A, Zini I, Tatemoto K, Pich, Hokfelt T, Mutt V, Terenius L. Central administration of neuropeptide Y induces hypotension bradypenea and EEG synchronization in the rat. Acta physiol Scand 118(2) : 189-192. 1984
  17. Aguirre JA, Fuxe K, Hedlund P, Narvaez JA, Cintra A, Rosen L, Agrati LF. Neuropeptide Y /angiotensin Ⅱ interaction in central cardiovascular regulation of the rat. Brain Res 566 : 61-69, 1991
  18. Edvinsson L, Copeland JR, Emson P, Mculloch J, Uddman R. Nerve fibers containing neuropeptide Y in the cerebrovascular immunocytochemistry, radioimmunoassay and vasomotor effects. J Cerebr Bool Flow Metabol 7 : 45-57, 1987
  19. Hong M, Lis Fournier A, St-Pierre and Pellejer G. Role of neuropeptid Y in the regulation of tyrosine hydroxylase gene expression in rat adrenal glands. Neuroendocrinology 61 : 85-88, 1995
  20. Hlguchi H. Neuropeptide Y functions and biosynthesis as a peptidergic neurotransmitter and the regulation of neuron-specific expression of NPY gene. Folia phamacol Jpn 93 : 203-218, 1989
  21. Zeigler HP and Karten HJ. Brain mechanism and feeding behavior in the pigeon (Columba livia). I. Quinto-frontal structures. J. Comp. Neurol. 152, 59-82. 1972
  22. Denbow DM. Food intake control in birds. Neurosci. Biobehav. Rev. 9, 223-232. 1985
  23. Kuenzel WJ. Neuroanatomical substrates involved in the control of food intake. Poultry Sci. 68, 926-937. 1989
  24. Shimjzu T, Cox K and Karten HJ. Intratelencephalic projections of the visual wulst in pigeons (Columba livia). J Comp. neurol. 359, 551-572. 1995
  25. Walsh KM, Kuenzel WJ. Effect of sulfamethazine on sexual precocity and neuropeptide Y neurons within the tuberoinfundibular region of the chick brain. Brain Res Bull, 1997, 44:6, 707-13
  26. Fraley GS and Kuenzel WJ. Precocious puberity in chicks (Gallus domesticus) induced by central injections of neuropeptide Y. Life Sci. 52, 1649-1656. 1993
  27. Chronwall BM, DiMaggio DA, Massari VJ, Pickel VM, Ruggiero DA, O'Donohue TL. The anatomy of neuropeptide Y-containing neurons in rat brain, Neuroscience 1985 Aug;15(4):1159-81
  28. Yano J. The EEG response to repetitive photic stimulation in various regions of the chicken brain. Electroencephalogr Neurophysiol, Mar, 40:3, 244-52. 1976
  29. Allen JM, McGregor GP, Woodhams PL, Polak JM, Bloom SR. Ontogeny of a novel peptide, neuropeptide Y (NPY) in rat brain. Brain Res, Jun, 303:1, 197-200.1984
  30. Chiba A. Immunohistochemical distribution of neuropeptide Y-related substance in the brain and hypophysis of the arctic lamprey, Lethenteron japonica. Brain Behav Evol, 1999, 53:2, 102-9
  31. Karten HJ, Revzin AM. The afferent connections of the nucleus rotundus in the pigeon. Brain Res, Oct, 2:4, 368-77 1966
  32. Kosareva AA, Ozirskaia EV, Tumanova NL, Gaidaenko GV. Structural organization of the Nucleus rotundus in the turtle Emys orbicularis. Zh Evol Biokhim Fiziol, Mar, 9:2, 195-201. 1973
  33. Ehrlich D and Mark R. An atlas of the primary visual projections in the brain of the chick Gallus. J. Comp.Neurol. 223, 592-610. 1984
  34. Tatemoto YK, Cartquist M and Mun V. Neuropeptide Y a novel brain peptide with structural simmilaritics to peptide YY and pancreatic polypeptide. Nature 296-670,1982