대한수의학회지 (Korean Journal of Veterinary Research)

  • 제42권2호
  • /
  • Pages.171-181
  • /
  • 2002
  • /
  • 2466-1384(pISSN)
  • /
  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지

시료고체상분산처리와 액체크로마토그라피를 이용한 소의 근육, 간 및 천엽에서의 벤지미다졸계 구충제 잔류분석

Matrix solid phase dispersion isolation and high performance liquid chromatographic determination of five benzimidazole anthelmintics in bovine muscle, liver and omasum

  • 김충희 (경상대학교 수의과대학(동물의학연구소)) ;
  • 김곤섭 (경상대학교 수의과대학(동물의학연구소)) ;
  • 박정희 (경상대학교 수의과대학(동물의학연구소)) ;
  • 하대식 (경상남도 보건 환경 연구원) ;
  • 류재두 (국립수의과학 검역원 부산지원) ;
  • 손성기 (경상남도축산진흥연구소) ;
  • 허정호 (경상남도 축산진흥연구소 남부지소) ;
  • 정명호 (경상남도 축산진흥연구소 남부지소) ;
  • 김종수 (경상대학교 수의과대학(동물의학연구소))
  • Kim, Chung-Hui (College of Veterinary Medicine, Gyeongsang National University(Institute of Animal Medicine)) ;
  • Kim, Gon-Sup (College of Veterinary Medicine, Gyeongsang National University(Institute of Animal Medicine)) ;
  • Park, Jung-Hee (College of Veterinary Medicine, Gyeongsang National University(Institute of Animal Medicine)) ;
  • Hah, Dae-Sik (Gyeongnam Provincial Government Institute of Health and Environment) ;
  • Ryu, Jae-Doo (National Veterinary Research and Quarantine Service Pusan Regional Office) ;
  • Son, Sung-Gi (Gyeongnam Livestock Promotion Institute) ;
  • Heo, Jung-Ho (Gyeongnam Livestock Promotion Institute South-branch) ;
  • Jung, Myung-Ho (Gyeongnam Livestock Promotion Institute South-branch) ;
  • Kim, Jong-Shu (College of Veterinary Medicine, Gyeongsang National University(Institute of Animal Medicine))
  • 심사 : 2002.04.19
  • 발행 : 2002.06.29
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Simultaneous multiresidue analysis using liquid chromatography determination for five benzimidazole anthelmintics(thiabendazole, oxibendazole, albendazole, mebendazole and fenbendazole) in bovine muscle, liver and omasum has been described. Blank or benzimidazole-fortified samples(0.5g) were blended with bulk $C_{18}$($40{\mu}m$, 18% load, endcapped, 2g). A column made from the resultant $C_{18}$/animal tissue matrix was first washed with hexane($8m{\ell}$), following which the benzimidazoles were eluted with acetonitrile($8m{\ell}$). Analytes of extracted sample were determined by liquid chromatography with UV detector at 290nm. Correlation coefficients of standard curves for individual benzimidazole isolated from fortified samples, using internal standardization, were linear($0.991{\pm}0.007$ to $0.996{\pm}0.005$) with average relative percentage recoveries from $62.1{\pm}3.8(%)$ to $92.3{\pm}7.5(%)$ for the concentration range($0.2{\sim}6.4{\mu}g/g$), respectively. Recoveries rates of TBZ, MBZ in liver, OBZ, MBZ in muscle and TBZ, MBZ in omasium from fortified benzimidazole were 92.%, 87.3%, 74.5%, 82.7%, 75.2% and 83.5% at condition II, respectively. Condition II showed higher recoveries rates than condition I. These results indicated that the matrix solid phase dispersion(MSPD) methodology is acceptable for the determination of 5 benzimidazole anthelmintics and may also suitable for other matrixes of food animal origin.

키워드

참고문헌

  1. 하대식, 김종수, 김곤섭, HPLC에 의한 계육의 설파메타진 잔류량 분석. 대한수의학회지. 34:55-62, 1994
  2. 하대식, 김종수, 김곤섭. MSPD와 HPLC를 이용한 어류의 잔류 설파제와 테트라사이클린계 항생물질의 동시 분석. 한국 식품위생 학회지, 5(3):139-158. 1997
  3. 김종수, 김곤섭, 하대식. 수입 축산식품에서의 잔류농약 동시분석 개발에 관한 연구. 대한수의학회지, 37(1) :213-220. 1997
  4. Delatour P. Parish R. Benzimidazole anthelmintics and related compounds: Toxicity and avaluation or residues. In Drug Residues in Animals. Rico, A. G., Ed., Academic Press: Orlando, FL, 175-204. 1986
  5. David LB, Ronald GB, Anne HB, and William CM. Monoclonal antibody for multiresidue ELISA of benzimidazole anthelmintics in liver. J Agric Food Chem, 42(7) :1588-1594, 1994
  6. Long AR, Hsieh LC, Malbrough MS. et al. Matrix Solid Phase Dispersion Isolation and Liquid Chromatographic Determination of five beznimidazole anthelmintics in fortified beef liver. J Assoc Off Anal Chem, 73(6) : 860-863. 1990
  7. Leen CD. Banzimidazole fungicides : Mechanism of action and biological impact. Ann Rev Phytopathol, 24:43-65. 1986
  8. Cares C, Marc. Disposition of netobimin, albendazole and its metabolites in the pregnant rat : Developmental toxicity. Toxicology and Applied Pharmacology, 144 : 56-61. 1986
  9. Malisch R, Bourgeois B, Lippold R. Multiresidue analysis of selected chemotherapeutics and antiparasities. Deut Lebensm-Rundsch, 88(7) 205-216. 1992
  10. Canon P, De La Plaza JL, Munoz-delgado L. Determination and persistence of several fungicides in post-harvest apples during their cold storage. J Agri Food Chem 35 : 114-148. 1987
  11. Susan EP, Charrlotte EF, Stephan AB. Determination of concentration of albendazole sulfoxide in plasma and uterine fluid of heifers. AJVR, 158 : 62-65. 1997
  12. Yess NJ, Gunderson EL, Roy RR. US Food and Drug Administration mornitoring of pesticide residues in infant foods and adult foods eaten by infants-children. Journal of the AOAC Interna, 76 : 492-507. 1993
  13. Delatour P, Parish R, Gyurik RJ. Albendazole : a comparison of relay embrytoxicity with embryotoxicity of individual metablites. Ann Research Vet, 12:159-167. 1981
  14. WHO. Tiabendazile(TBZ) in Toxicological Evalution of certain Veterinary Drug Residues in Food. WHO Food Additives. Vol. 31. WHO, Geneva, Switzerland. 37-62. 1993
  15. U. S. Department of Administration. General Principles for Evaluating the Safety of Compounds Used in Food-producing Animals, Washington, DC. 1986
  16. U. S. Department of Agriculture. Compound evalution and analytical capability national residue program plan, Food Safety and Inspection Service, Brown, J.(Ed.), Washington, DC. 1988
  17. U. S. Department of Administration, Fenvendazole drugs for animal use, Code of Federal Regulations. Title 21, Part 556:730, 1988
  18. 농림수산부. FAO.WHO, 국제식품 규격위원회, 축산식품 위생 지침(동물 약품 잔류, 식육 위생),p 7. 1994
  19. Long AR, Hsieh LC, Malbrough MS, et al. Matrix solid-phase dispersion(MSPD) extraction and liquid chromatographic determination of five benzimidazole anthelmintics in pork muscle tissue. J Food Comos annal, 3 : 20-26. 1990
  20. Marti AM, Mooser AE, Koch H. Determination of benzimidazole anthelminitics in meat samples. J Chromatogr, 498 : 145-157. 1990
  21. Barker SA, Long AR, Hines ME. II. Disruption and fractionation of biological materials by matrix solid phase dispersion. J Chromatogr, 629 : 23-24. 1993
  22. Barker SA, Long AR, Short CR. Isolation of drug residues from tissues by solid phase dispersion. J Chromatogr 475 : 353-361. 1989
  23. Boulaire SL, Bauduret JC, Andre F. Veterinary Drug Residues Survey in Meat : An HPLC Method with a Matrix Solid Phase Dispersion Extraction. J Agric Food Chem; 45 : 2134-2142. 1997
  24. Barker SA, McDowell T, Charkhian B, et al. Methodology for the Analysis of Benzimidazole Anthelmintics as Drug Residues in Animal TISSues. J Assoc Off Chem; 73(1) : 22-25. 1990
  25. Barker SA, McDowell T, Charkhian B, et al. Methodology for the Analysis of fenbendazole and its metabolites in plasIm, urire, feces, and homogenates. Anal Biochem, 155 : 112-118. 1986
  26. Bogan JA, Marriner S. Analysis of benzimidazoles in body fluids by high performance liquid chromatography. J Pharm Sci, 69 : 422-423. 1980
  27. Rene VA and Neslon AJ. Liquid Chromatographic fluorescenece method for multiresidue detennination of thiabendazole 5-hydroxy-thiabendazole in milk. Journal of AOAC lnterna Tion, 78(3) : 642-646. 1995
  28. Susas SC, Tai NC, Charlie JB. Determination of thiabetdlzole, 5-hydroxythiabendazole, fenbetdlzole, and oxfendazole in milk. J Assoc off Anal Chem, 73(3) : 368-37. 1990