Lipid Studies of Carum Roxburghianum Seeds

  • Waheed, Amran (Applied Chemistry Research Centre, PCSIR Laboratories Complex) ;
  • Mahmud, Shahid (Applied Chemistry Research Centre, PCSIR Laboratories Complex) ;
  • Saleem, Muhammad (Applied Chemistry Research Centre, PCSIR Laboratories Complex) ;
  • Yamin, Muhammad (Applied Chemistry Research Centre, PCSIR Laboratories Complex) ;
  • Khan, Muhammad Naeem (Applied Chemistry Research Centre, PCSIR Laboratories Complex)
  • Published : 2003.09.01

Abstract

Total lipids extracted from the powdered seeds of Carum roxburghianum were fractionated into hydrocarbons (0.30%), wax esters (0.30%), sterol esters (1.35%), triacylglycerols (72.41%), free fatty acids (6.06%), 1,3-diacylglycerols (4.60%), 1,2- diacylglycerols (0.64%), glycolipids (5.10%), sterols (1.20%), 2-monoacylgylcerols (3.18%), 1-monoacylglycerols (1.46%), phosphatidylethanolamines (1.08%) phosphatidylcholines (0.40%), lysophosphatidylethanolamines (1.48%) and phosphatidylinositols (0.44%) with the help of TLC. The fatty acid composition of all the lipid fractions was determined after converting them into their methyl esters with $BF_3-methanol$ reagent and then analyzing them by GC. Oleic acid was found as a major component in all the lipid classes, whereas palmitic, linoleic and linolenic acids were present in lesser quantities. Arachidic acid was identified as a minor component in only seven out of twelve lipid classes.

Keywords

References

  1. Akhtar, W. M., Nahid, K, Nadeem, N. M. and Zahid, R., Variation in the composition of polar and non polar lipids and their fatty acids in the germinating seeds of Cucumis melo. Pak. J. Bio. Chern. XVI 71-81 (1981)
  2. Akhtar, W. M., Zafar, M. I. and Nadeem, N., Lipid class and Fatty acid composition of Pumpkin seed oil. Pak. J. Sci. Research. 32, 295-300 (1980)
  3. Bhatty, M. K, 'Essential Oils of the plant family Umbelliferae', Final Report, PL-480, PCSIR Labs. Complex, pp. 29-46, Lahore, Pakistan (1977)
  4. Chaudri, T. A, Ahmad, I., Yamin, M. and Mahmud, S., The distribution of fatty acids at 1,2 and 3 position in the triacylglycerol of Carum roxburghianum seed, Pro. Pakistan Acad. of Sci., 36(2), 159-164, (1999)
  5. Colowick, S. P. and Kaplan, N. O., Methods in Enzmology, Vol.XIV, p. 544, Academic press London, 1969a
  6. Colowick, S. P. and Kaplan, N. O., Methods in Enrmology, Vol. XIV, p. 546, Academic press London, 1969b
  7. Colowick, S. P. and Kaplan, N. O., Methods in Ensmology, Vol. XIV, p. 547, Academic press London, 1969c
  8. Folch, J. Lees, M. and Stanley, S., A simple method for the isolation and purification of total lipid from animal tissues. J. Bioi. Chern. 226,497-509 (1957)
  9. Kirtikar, K R. and Basu, B. D., Indian Medicinal Plants, 2nd ed., Vol. 11, pp. 1190-1231, Lalit Mohan Basu, Allahabad, India (1984)
  10. Morrison, W. R. and Smith, L. M., Preparation of fatty acid methyl esters and dimethyl acetals from lipids with boron fluoride methanol, J. Lipid. Research. 5, 600-608 (1984)
  11. Stahl, E., Thin layer chromatography, George Allen and Unwin Ltd., London, p. 60-67 (1969)