Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Growth Inhibitory Effects of Sesamolin from Sesame Seeds on Human Leukemia HL-60 Cells

참깨에서 분리된 세사몰린의 백혈병 세포주 HL-60 생장억제 효과

  • Kim, Kwan-Su (Dept. of Medicinal Plant Resources, School of Biotechnology & Resources, Mokpo National University) ;
  • Kang, Sam-Sik (Natural Products Research Institute and College of Pharmacy, Seoul National University) ;
  • Ryu, Su-Noh (Dept. of Agricultural Science, Korea National Open University)
  • 김관수 (목포대학교 생물산업학부 생약자원전공) ;
  • 강삼식 (서울대학교 약학대학/천연물과학연구소) ;
  • 류수노 (한국방송통신대학교 농학과)
  • Published : 2003.09.30
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Abstract

This study was carried out to test the growth inhibitory effects of sesamolin obtained from sesame seeds. Sesamolin inhibited the growth of human leukemia HL-60 cells in cultures and the synthesis of macromolecules in dose- and time-dependent manners. Sesamolin in the $60{\sims}100\;{\mu}g/ml$ range was cytostatic. At concentrations greater than $200\;{\mu}g/ml$ sesamolin was cytocidal to HL-60 cells and at $60\;{\mu}g/ml$ inhibited the synthesis of DNA, RNA and protein in HL-60 cells by 35.1, 6.1, and 5.3%, whereas at $200\;{\mu}g/ml$ these inhibitions were 86.8%, 81.5% and 96.7%, respectively. The inhibitory effect of sesamolin on DNA synthesis was irreversible.

본 연구는 참깨 종자로부터 추출, 분리된 세사몰린의 백혈병 HL-60 세포의 생장억제에 미치는 영향을 조사하였다. 세사몰린은 농도와 시간 의존적으로 HL-60 세포의 생합성을 억제하였다. $60{\sim}100\;{\mu}g/ml$ 농도의 세사몰린 범위에서 사포증식이 억제적이었다. $200\;{\mu}g/ml$ 이상의 세사몰린 농도에서 HL-60 세포에 세포파괴성으로 나타났다. 그리고 $60\;{\mu}g/ml$에서 HL-60 세포의 DNA, RNA, 단백질의 합성억제 정도는 35.1%, 6.1% 5.3%였다. 반면에 $200\;{\mu}g/ml$에서의 억제 정도는 각각 86.8%, 81.5%, 96.7%였다. DNA 합성에 대한 세사몰린의 억제효과는 비가역적이었다.

Keywords

References

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