Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Characteristics of the Pigments Extracted from Grains of Colored Barley

유색 보리 종실의 품종별 색소 특성

  • Ju, Wan-Taek (Department of Agricultural Chemistry, Chonnam National University) ;
  • Min, Kyung-Soo (Department of Agronomy, Chonnam National University,Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Park, Ro-Dong (Department of Agricultural Chemistry, Chonnam National University)
  • 주완택 (전남대학교 농업생명과학대학 응용생물공학부) ;
  • 민경수 (전남대학교 농업생명과학대학 응용식물학부,농업과학기술연구소) ;
  • 박노동 (전남대학교 농업생명과학대학 응용생물공학부)
  • Published : 2003.11.30
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Abstract

The characteristics of the pigments extracted from grains of the colored barley varieties Ab2231, Mozzimugi, Lion, and Hogye 4 were studied. Extraction of the pigments from powder (80 mesh) of the barley grains was most efficient in 80% ethanol containing 1.0% HCI for 1 hr at $60^{\circ}C$. The pigments were also stable in 80% ethanol containing 1.0% HCI. Depending on the absorption spectra, maximal peak wavelength, and the ratio of $A_{440}/A_{max}$, the varieties were separated into two groups, colored naked barley (Ab2231 and Mozzimugi) and colored covered barley (Lion and Hogye 4).

유색보리 4품종과 보통보리 4품종 종실의 종피에 함유되어 있는 색소의 특성을 분석하여 해당작물의 신품종 육성시 우수계통 선발에 유용한 자료를 제공코자 하였다. 유색(검정)쌀보리인 Ab2231을 표준시료로 하여 실험한 결과 에탄올의 농도가 80%일 때 색소추출 효율이 양호하였다. 80% 에탄올에 1.0% 염산을 첨가한 것이 추출효율이 가장 좋았다. 추출시간에 따른 추출효율성을 조사한 결과 1시간 추출이면 충분하였다. 추출된 색소의 안정성을 유지하기 위하여 첨가시키는 산의 적정농도를 알아본 결과 1.0% HCI을 첨가한 농도에서 가장 낮은 색소량 감소율이 나타났다. 각 보리 품종 시료에서 추출한 색소의 흡광스펙트럼을 조사하였던 바, Ab2231의 그것은 535 nm에서 최대흡광파장을 나타내어 안토시아닌계 색소로 판단되었으며, 호계4호의 그것은 안토시아닌계 이외의 색소를 주로 함유하는 것으로 보였다. 한편, 추출된 색소의 특성에 따라 유색보리의 품종은 Ab2231와 모찌무기로 대표되는 쌀보리 그룹과, Lion과 호계4호로 대표되는 겉보리 그룹으로 분류되었다.

Keywords

References

  1. Hwang, I. K. and Ahn, S. Y. (1975) Studies on the anthocyanins in wild vines (Vitis amurensis Ruprecht). 2. Identification of anthocyanins in wild vines. J. Korean Soc. Agric. Chem. Biotechnol. 18, 188-193
  2. Kim, K. I., Nam, J. H. and Kwon, T. W. (1973) On the proximate composition, organic acids and anthocyanins of Omija, Schizandra chinensis Baillon. Korean J. Food Sci. Technol. 5, 178-182
  3. Koppen, B. H. and Baxxon, D. S. (1966) The anthocyanin pigments of Barinka grape. Phytochemistry 5, 183-187 https://doi.org/10.1016/S0031-9422(00)85097-9
  4. Hrazdina G. (1970) Column chromatograpgic isoladon of the anthocyanidin-3,5-diglucosides from grapes. J. Agric. Food Chem. 18, 243-245 https://doi.org/10.1021/jf60168a009
  5. Oh, S. K., Choi, H. C., Cho, M. Y. and Kim, S. U. (1996) Extraction method of anthocyanin and tannin pigments in colored rice. J. Korean Soc. Agric. Chem. Biotechnol. 39, 327-331
  6. Williams, M. and Hrazdina, G. (1978) High pressure liquidchromatographic separadon of 3-gu1cosides, 3,5-dig1ucosides, 3-(6-0-p-coumary1)g1ucosides and 3-(6-0-p-coumary1g1ucoside)-5-g1ucosides of anthocyanidins. J. Chmmatogro 155, 389-398 https://doi.org/10.1016/S0021-9673(00)88000-0
  7. Kim, S. L., Kim, E. H., Son, Y. K. Song, J. C., Hwang, J. J.and Hur, H. S. (1999) IdentiScadon of anthocyamn pigments inblack waxy com kemels. Korean J. Breed 31, 408-415
  8. Yoon, H. H., Paik, Y. S., Kim, J. B. and Hahn, T. R. (1995) Identification of anthocyanins from Korean pigmented rice. J.Korean Soc. Aeric. Chem. Biotechnot. 38, 581-583
  9. Cho, M. H., Paik, Y. S., Yoon, H. H. and Hahn, T. R. (1996) Chemical structure of the major color component from a Korean pigmented lice vahety. J. Korean Soc. Agric. Chem. Biotechnol. 39, 304-308
  10. Choung, M. G., Baek, I. Y, Kang, S. T., Han, W. Y, Shin, D.C., Moon, H. P. and Kang, K. H. (2001) Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max (L.) Merr.). J. Agric. Food Chem. 49, 5848-5851 https://doi.org/10.1021/jf010550w
  11. Dao, L. T., Takeoka, G. R., Edwards, R. H. and Berrios, J. J. (1998) Improved method for the stabilization of anthocyanidins. J. Agric. Food Chem. 46, 3564-3569 https://doi.org/10.1021/jf980359v
  12. Deguchi, T., Shohara, S., Ohba, R. and Ueda, S. (2000) Effects of pH and light on the storage stability of the purple pigment, Hordeumin, from uncooked barley bran fermented broth. Biosci. Biotechnol. Biochem. 64, 2236-2239 https://doi.org/10.1271/bbb.64.2236
  13. Tsukui, A., Suzuki, A., Nagayama, S. and Terahara, N. (1996) Stability of anthocyanin pigments from purple leaves of Perilla ocimoides L. var. Crispa. Nippon Shokuhin Kagaku Kaishi 43, 113-119
  14. Kim, S. L., Hwang, J. J., Song, J., Song, J. C. and Jung, K. H. (2000) Extraction, Purification, and quandfication of anthocyanins in colored rice, black soybean, and black waxy com. Korean J. Breed 32, 146-152
  15. Harborne, J. B. and Self, R. (1987) Malonated cyanidin-3-glucosides in Zea mays and other grasses. Phytochemistry 26, 2417-2418 https://doi.org/10.1016/S0031-9422(00)84738-X
  16. Park, S. Z., Kim, H. Y., Han, S. J. and Ryu, S. N. (2000) Cyanidin 3-glucoside content in Fl, F2 and F3 grains of pigmented rice Heugjinjubyeo crosses. Korean J. Breed 32, 285-290
  17. Deguchi, T., Yoshimoto, M., Ohba, R. and Ueda, S. (2000) Antimutagenicity of the purple pigment, Hordeumin, from uncooked barley bran-fermented broth. Biosci. Biotechnol. Biochem. 64, 414-416 https://doi.org/10.1271/bbb.64.414
  18. McMurrough, I., Loughery, M. J. and Hennigan, G. P. (1983) Content of (+)-cathechin and proanthocyanidins in barley and malt grain. J. Sci. Food Agric. 34, 62-72 https://doi.org/10.1002/jsfa.2740340110
  19. Ohba, R., Kitaoka, S. and Ueda, S. (1993) Properties and precusors of Hordeumin produced from uncooked barley bran by ethanol fermentation. J. Ferment. Bioeng. 75, 121-125 https://doi.org/10.1016/0922-338X(93)90222-T
  20. Tsukui, A., Suzuki, A., Hayashi, K., Nishiyama, R. and Ohara, N. (1996) Change in anthocyanin pigments during alcohol fermentation of a commercially available apple juice. Nippon Shokuhin Kagaku Kaishi 43, 1128-1132 https://doi.org/10.3136/nskkk.43.1128
  21. Nam, S. H. and Kang, M. Y. (1997) In vitro inhibitory effect of colored rice bran extracts carcinogenicity. J. Korean Soc. Agric. Chem. Biotechnol. 40, 307-312
  22. Tamagawa, K., Fukushima, S., Kobori, M., Shinmoto, H. and Tsushida, T. (1998) Proanthocyanidins from barley bran potentiate retinoic acid-induced granulocydc and sodium butyrate-induced monocytic differentiation of HL60 cells. Biosci. Biotechnol. Biochem. 62, 1483-1487 https://doi.org/10.1271/bbb.62.1483