Effects of Flow Rate of Feed Kanjang and Volume Reduction Ratio of Retentate on the Permeate Flux and Rejection of Microbes Cells and Components in Kanjang during Ultrafiltration Operations

재래식 간장의 한외여과시 공급액의 주입속도와 잔류액의 용량감소율이 간장의 투과유속, 미생물균체 및 성분저지율에 미치는 영향

  • Kwon, Kwang-Il (Department of Food Science and Technology, Yeungnam University) ;
  • Lee, Jong-Gu (Department of Food Science and Technology, Yeungnam University) ;
  • Choi, Jong-Dong (Daegu Regional Food and Drug Administration) ;
  • Chung, Hyun-Chae (Department of Food Science and Technology, Yeungnam University) ;
  • Im, Moo-Hyeog (Korea Food and Drug Administration) ;
  • Kim, Ki-Ju (Department of Food Science and Technology, Yeungnam University) ;
  • Kim, Woo-Seong (Busan Regional Food and Drug Administration) ;
  • Sung, Jun-Hyun (Kyungin Regional Korea Food and Drug Administration) ;
  • Kwon, O-Jun (Department of Food Science and Technology, Yeungnam University) ;
  • Kim, Young-Ji (Department of Food Nutrition, Yeongnam College of Science and Technology) ;
  • Suh, Chung-Sik (Department of Food Nutrition, Yeongnam College of Science and Technology) ;
  • Choi, Cheong (Department of Food Science and Technology, Yeungnam University) ;
  • Choi, Kwang-Soo (Department of Food Science and Technology, Yeungnam University)
  • 권광일 (영남대학교 식품가공학과) ;
  • 이종구 (영남대학교 식품가공학과) ;
  • 최종동 (대구식품의약품안전청) ;
  • 정현채 (영남대학교 식품가공학과) ;
  • 임무혁 (식품의약품안전청) ;
  • 김기주 (영남대학교 식품가공학과) ;
  • 김우성 (부산식품의약품안전청) ;
  • 성준현 (경인지방식품의약품안전청) ;
  • 권오준 (영남대학교 식품가공학과) ;
  • 김영지 (영남이공대학 식품영양학과) ;
  • 서정식 (영남이공대학 식품영양학과) ;
  • 최청 (영남대학교 식품가공학과) ;
  • 최광수 (영남대학교 식품가공학과)
  • Published : 2003.02.01

Abstract

Ultrafiltration (UF) tests performed on traditional soy sauce (kanjang) using UF flat membrane test cell unit with thin film laminar flow similar to the spiral type membrane module in batch operations revealed reduction in permeate flux is proportional to the logarithm values of volume reduction ratio of the retentate kanjang at different feed rate of kanjang. Feed rate of 1.5 L/min was found to be adequate for long-term UF operation of kanjang using the test unit attached with MW cut-off size of 200,000 dalton polyoleffin plastic membrane in batch operation with the least concentration polarization. The higher the feed rate of kanjang, the lower the permeability of total nitrogen and NaCl, resulting in lower optical density at 500 nm and lower permeability of minerals such as Cu, Mn, and Mg. Microbial cells were completely rejected regardless of the feed rate, whereas most free amino acids were not.

회분처리법으로 박층류식 평막 test cell에 의한 재래간장의 한외시험을 실시한 결과 공급액 주입속도별 투과유속의 감소는 용량감소율의 대수값에 비례하여 감소하였고 본 연구에서 사용한 분획분자량 200,000 dalton의 막에 의한 재래간장의 한외여과에서는 1.5L/min의 유속으로 공급액을 주입하는 것이 농도분극화현상을 최소화하면서 장시간 작업할 수 있음을 알 수 있었다. 간장의 한외여과시험에서 간장내의 총질소와 소금성분은 투과유속이 빠를수록 막에 의한 저지율이 더 높아졌고 따라서 500 nm에서의 간장의 흡광도는 더 낮아졌으며 Cu, Mn 및 Mg와 같은 무기성분도 다소 저지되었다. 미생물 균체는 공급간장의 유속에 상관없이 분획분자량 200,000 dalton의 한외여과막으로 100% 저지되었으나 유리아미노산은 거의 저지되지 않아 투과됨을 알 수 있었다.

Keywords

References

  1. Chang, K.S. Application of membrane separation technology in fermented foods. Food Sci. Ind. 32: 2-13 (1999)
  2. Kusudo, S., Hamamoto, Y. and Miyake, T. Application of membrane to soy sauce (Part 2)-Filtration of soy sauce precipitates by SF filter. J. Japan Soy Sauce Res. Inst. 7: 108-111 (1981)
  3. Sakamoto, A.H., Hiraguri, Y.R., Myrakami, T., Kanematsu, Y.N. and Motogawa, T.Y. Ultrafiltration of sterilized shoyu by membrane Process, J. Japan Soy Sauce Res. Inst. 12: 81,94 (1986)
  4. Sasaki, S., Nishibe, M., Noguchi, K. and Inamori, K. Studies on membrane filtration of soy sauce (Part I), Membrane filtration of raw soy sauce. J. Japan Soy Sauce Res. Inst. 19: 9-16 (1993)
  5. Choi, J.D., Kwon, K.I., Chung, H.C., Im, M.H., Choi, C. and Choi, K.S. Effects of molecular weight cut-off size of membrane on the flux and quality of permeate during ultrafiltration of Korean conventional soy sauce, Food Eng. Prog. 4: 55-60 (2000)
  6. Ha, S.D. Evaluation of dryfilm method for isolation of microorganisms from foods. Korean J. Appl. Microbiol. Biotechnol. 24: 178-184 (1996)
  7. AOAC. Official Methods of Analysis. 16th ed., Association of Official Analytical Chemists, Washington, DC, USA (1995)
  8. Park, S.K., Han, S.O., Kyung, K.H. and Yoo, Y.J. Effect of oxygen on the browning of soy sauce during storage. Korean J. Food Sci. Technol. 22: 307-311 (1990)
  9. Yoda, K., Urakabe, R. and Tsuchida, T. Enzyme electrode provided with immobilized enzyme membrane. U.S. Patent 4,240,889 (1980)
  10. Japanese Soy Sauce Research Institute. Methods in Shoyu Experiments, pp. 140-150. Mitsuosa Printing Co., Tokyo, Japan (1990)
  11. Sin, H.S. Food Analysis (Theory and Experiments), pp. 113-130. Sinkwang Publishing Co., Seoul (1989)
  12. Sanbishi Co. Ltd. and Arubakku service Co. Ltd. Application of membrane technology in the filtration of brewed soy sauce, pp. 479-502. In: Membrane Utilization Technology in Food Processing. Kimura, S. et al. (eds.). Cooperation of Membrane Technology Utilization in Food Industry,Tokyo, Japan (1987)