Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation and Identification of Quercetin with Antioxidative Activity from the Fruits of Rubus coreanum Miquel

복분자 열매에서 항산화활성을 지닌 quercetin의 분리 및 동정

  • Yoon, In (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Wee, Ji-Hyang (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Moon, Jae-Hak (Department of Applied Biology and Chemistry, The Research Institute of Agricultural Resources Development, Konkuk University) ;
  • Ahn, Tae-Hoe (Research Center, Ottogi Food Co., Ltd.) ;
  • Park, Keun-Hyung (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University)
  • 윤인 (전남대학교 식품공학과 및 농업과학기술연구소) ;
  • 위지향 (전남대학교 식품공학과 및 농업과학기술연구소) ;
  • 문제학 (건국대학교 응용생물화학과.농업자원개발연구소) ;
  • 안태회 (오뚜기식품 중앙연구소) ;
  • 박근형 (전남대학교 식품공학과 및 농업과학기술연구소)
  • Published : 2003.06.01
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Abstract

The methanol (MeOH) extracts from the fruits of Rubus coreanum showed antioxidative activity. The antioxidative substance in MeOH extracts was successively purified with solvent fractionation, adsorption chromatography, and gel filtration. The purified active substance was isolated by HPLC and identified as quercetin by EI-MS, and $^1H-NMR$ analyses. The amount of quercetin was 0.25 mg per 100 g in fresh fruits of Rubus coreanum Miquel.

복분자 열매에 함유된 항산화활성 물질을 탐색하기 위하여 MeOH로 추출하고, 이 추출물을 용매분획하여 EtOAc가용 중성획분을 얻었다. 이 획분에 함유된 항산화활성 물질을 흡착성과 분자체 효과를 이요한 Sephadex LH-20 column chromatography로 정제하고, HPLC에 의해 1종의 항산화활성물질을 단리하였다. 분리된 활성물질의 구조 해석을 위해 EI-MS와 $^1H-NMR$ 분석을 실시하여, 활성물질은 구조식이 $C_{15}H_{10}O_7$이며 분자량이 302인 quercetin으로 동정되었다. 또한 항산화활성 물질 quercetin은 HPLC에 의해 복분자 열매 100g 중에 $0.25{\pm}0.02\;mg$이 함유되어 있는 것으로 분석되었다.

Keywords

References

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