Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Adipocyte Differentiation Inhibitor Isolated from the Barks of Phellodendron amurense

황백(Phellodendri Cortex)으로부터 분리한 지방세포 분화 저해물질

  • Kim, Kyung-Hee (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ahn, Soon-Cheol (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Myung-Sun (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kweon, Oh-Song (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Oh, Won-Keun (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Min-Soo (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Sohn, Cheon-Bae (Department of Food and Nutrition, Chungnam National University) ;
  • Ahn, Jong-Seog (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Published : 2003.06.01
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Abstract

For the development of the anti-obesity natural drug, the inhibitor of adipocyte differentiation was screened from Korean traditional medicinal plants. Phellodendri Cortex was selected as a candidate of adipocyte differentiation inhibitor. An inhibitory compound PC-4 was purified from the methanol (MeOH) extract of Phellodendri Cortex using silica gel and ODS RP-18 column chromatography and HPLC. PC-4 was obtained as yellow powder; UV ${\lambda}_{max}$ (MeOH): 230, 260, 340 and 430 nm. The chemical structure of PC-4 was determined as an isoquionoline alkaloid, berberine, on the basis of various NMR experiments including $^1H-\;and\;^{13}C-NMR$. The PC-4 inhibited the differentiation of preadipocyte NIH-3T3 L1 cells at a concentration of $1\;{\mu}g/mL$.

비만 저해 물질을 탐색하고자 전통의학에서 사용되는 200여 종류의 식용 또는 약용식물을 대상으로 지방세포 분화저해활성을 보이는 생약식물을 선별하여 용매층으로 활성이 이행되는 생약 중 황백(Phellodendri Cortex)을 최종적으로 후보식물로 선정하였다. 황백을 메탄올로 추출한 후, silica gel column chromatography, ODS RP-18 column chromatography, HPLC 등을 수행하여 지방세포 분화 저해활성을 갖는 화합물 PC-4를 분리하였다. PC-4는 노란색의 분말형태로 메탄올을 용매로 UV 최대흡수치를 조사한 결과, 222, 265, 349, 429 nm에서 최대의 UV의 흡수치를 보였으며 산이나 알카리 조건에서 최대 흡수치의 변화가 관찰되지 않았다. EI-mass spectrum을 측정한 결과, 분자량이 336.1로 예상되었으며 $^1H-NMR,\;^{13}C-NMR,\;DEPT,\;^1H-^1H\;COSY$, HMQC, HMBC NMR spectrum을 통해, PC-4는 isoquionoline alkaloide계열의 berberine으로 동정되었다. PC-4는 지방의 대사에 관여하는 중요한 효소인 fatty acid synthase와 pancreatic lipase에 대한 저해활성은 나타나지 않았으나, 지방세포 분화 저해활성은 $1\;{\mu}g/mL$의 낮은 농도에서도 관찰되었다.

Keywords

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