Effects of Fermentation Conditions on Production of Erythritol by Candida magnoliae

Candida magnoliae의 발효 조건이 erythritol의 생산에 미치는 영향

  • Choi, Jung-Hyun (Department of Food Science and Technology and Research Center for New Biomaterials in Agriculture, Seoul National University) ;
  • Kim, Myoung-Dong (Department of Food Science and Technology and Research Center for New Biomaterials in Agriculture, Seoul National University) ;
  • Seo, Jin-Ho (Department of Food Science and Technology and Research Center for New Biomaterials in Agriculture, Seoul National University) ;
  • Ahn, Jang-Woo (Department of Food and Biotechnology, Chungkang College of Cultural Industries)
  • 최정현 (서울대학교 식품공학과 및 농업생물신소재 연구센터) ;
  • 김명동 (서울대학교 식품공학과 및 농업생물신소재 연구센터) ;
  • 서진호 (서울대학교 식품공학과 및 농업생물신소재 연구센터) ;
  • 안장우 (청강문화산업대학 식품생명과학과)
  • Published : 2003.08.01

Abstract

This study was carried out to examine the effects of fermentation conditions on the production of erythritol by osmophilic yeast Candida magnoliae. It was found that sucrose was superior to glucose as carbon source and 109 g/L erythritol was produced from 400 g/L sucrose. When yeast extract was used as nitrogen source, maximum values of yield and productivity for erythritol were obtained at 15 and 20 g/L of yeast extract, respectively. A mixture of 15 g/L yeast extract and 3 g/L ammonium phosphate allowed more efficient utilization of sucrose and hence resulted in 149 g/L of erythritol, 0.37 g erythritol/g sucrose of erythritol yield and $0.78\;g/L{\cdot}hr$ of erythritol productivity. A batch fermentation supplemented with 40 g/L KCl resulted in an erythritol concentration of 167 g/L and an erythritol yield of 0.42 g erythritol/g sucrose.

내삼투압성 효모인 Candida magnoliae의 발효조건이 erythritol의 생산에 미치는 영향에 대해 조사하였다. 탄소원으로는 포도당보다 sucrose가 우수하였으며, sucrose 400 g/L를 주입하였을 때 109 g/L의 erythritol이 생산되었다. 질소원으로 yeast extract을 사용했을 때, 질소원의 농도가 낮을수록 높은 erythritol 수율을 획득할 수 있었으며, 15 g/L의 yeast extract와 함께 무기질소원인 ammonium phosphate를 3 g/L의 농도로 첨가하므로써, 수율 0.37 g/g, $0.78\;g/L{\cdot}hr$의 생산성으로 149 g/L의 erytnritol을 얻을 수 있었다. KCl을 이용한 높은 삼투압 조건에서의 플라스크 배양 결과, 40 g/L의 농도로 첨가한 경우가 erythritol 생산에 가장 유리하였으며, 이와 같은 실험결과를 발효기 배양에서 검증한 결과, KCl을 40 g/L로 첨가하여 167 g/L의 erythritol을 0.42 g erythritol/g sucrose의 수율로 생산할 수 있었다.

Keywords

References

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