한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

천마 분말, 에탄올 및 열수추출물이 고지방식이를 급여한 흰쥐의 혈청지질과 체지방에 미치는 영향

Effect of Whole Powder and Extracts of Gastrodiae Rhizoma on Serum Lipids and Body Fat in Rats Fed High-Fat Diet

  • 발행 : 2003.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

천마의 분말과 50% 에탄올 및 열수추출물이 돼지기름(lard 10%)을 첨가한 고지방식이를 급여한 흰쥐의 체중증가율, 혈청지질과 테스토스테론농도, 혈당치 및 정소상체지방패드(EFP) 등에 미치는 영향을 조사하였다. 시험기간 중 체중증가율은 식이군간 통계적인 차이는 없었지만 대조군(A, B)과 열수추출물군(G)이 높았고, 그 외 천마식이군은 비슷하였다. 장기무게는 식이군간에 통계적인 차이가 있었다(p<0.05). 혈청지질중 TC와 TG농도는 두 대조군이 가장 높았고, 열수추출물군(H)이 유의하게 낮았다(p<0.05). HDL농도는 천마식이군중 50% 에탄올추출물군(E)이 가장 높았고 A군이 가장 낮았다(p<0.05). LDL+VLDL농도는 A군이 가장 높았고 천마식이군에서는 50% 에탄올추출물군(E)와 열수추출물군(G, H)이 유의하게 낮았다(p<0.05). 테스토스테론농도는 식이군간에 통계적인 차이는 나타나지 않았지만 천마군중에서는 E군, D군 및 H군이 높았고 대조군을 포함한 다른 천마군은 비슷하였다. 혈당치는 B군과 G군이 식이군 중 통계적으로 가장 높았고 H군은 통계적으로 가장 낮았다(p<0.05). 체지방의 축적정도를 나타내는 EFP무게는 두 대조군이 천마식이군 보다 유의하게 높았고 분말군(C, D)과 열수추출물군(H)이 유의하게 낮았다(p<0.05). 이상의 결과로 볼 때 천마 분말 보다 50% 에탄올추출물 2 brix군(E)과 열수추출물 10 brix군(H)이 고지방식이를 급여함에도 혈청지질과 체지방의 축적을 감소시키는 것으로 나타났고 혈청 테스토스테론농도도 E군이 통계적인 유의성은 없었지만 식이군 중 가장 높았다.

Effects of Gastrodiae Rhizoma on serum lipids and body fat in rats fed high-fat diet supplemented with 10% (w/w) lard during 11-week experimental period were investigated. Ninety-six male rats were randomly divided into eight groups: A, negative control (lard 10%); B, positive control (lard 10%+rat chow+5 brix water extract); C, lard 10%+1% G. Rhizoma powder; D, lard 10%+5% G. Rhizoma powder; E, lard 10%+2 brix 50% ethanol extract; F, lard 10%+10 brix 50% ethanol extract; G, lard 10%+2 brix water extract; H, lard 10%+10 brix water extract. Body weight gain, although slightly higher in groups A, B, and G, did not differ significantly among dietary groups. Weights of liver, spleen, kidney, and testis were significantly higher among dietary groups. Serum total cholesterol and triglyceride concentrations were markedly higher in both control groups than group H (p<0.05). Serum HDL concentration was significantly higher in group E, and lower in group A (p<0.05). Serum LDL+VLDL concentration was significantly lower in groups E, G, and H (p<0.05). Serum testosterone level was slightly higher in groups E, D, and H. Blood glucose level was significantly higher in groups B and G, whereas lower in group H (p<0.05). Epididymal fat pad (EFP) was markedly higher in control groups than G. Rhizoma diet-fed groups (p<0.05). The results indicated that decrease in serum lipids (TC, TG, LDL+VLDL) and body fat, and increase in serum HDL and testosterone levels were higher in groups E and H extracts than groups fed G. Rhizoma powder.

키워드

참고문헌

  1. Society of Oriental Medicine. The Modern Oriental Medicine, pp. 446-447. Hakchang-Sa, Seoul, Korea (1993)
  2. Ku, B.H. Experimental studies on the pharmaceutical effects of Gastrodiae elata. M.S. thesis, Kyung-Hee Univ., Seoul, Korea (1991)
  3. Huang, J.H. Comparison studies on pharmacological properties of injection Gastrodia elata, gastrodin-free fraction and gastrodin. Chung-Kuo-Hsueh-Ko-Hsueh-Yuan-Hsueh-Pao 11: 147-152 (1989)
  4. Huang, Z.L. Recent developments in pharmacological study and clinical application of Gastrodia elata in China. Chung-Hsi-I-Chieh- Ho-Tsa-Chih 5: 251-258 (1985)
  5. Wu, H.Q., Xie, L., Jin, X.N., Ge, Q., Jin, H. and Liu, G.Q. The effect of vanillin on the fully amygdala-kindled seizures in the rat. Yao-Hsueh-Hsueh-Pao 24: 482-489 (1989)
  6. Paik, Y.S., Song, J.K., Yoon, C.H., Chung, K.S. and Yun-Choi, H.S. Anti-platelet and anti-thrombotic effects of Gastrodia elata. Korean J. Pharmacogn. 26: 385-389 (1995)
  7. Kim, E.J., Ji, G.E. and Kang, Y.H. Effects of Gastrodia Rhizoma extracts on global coronary circulation in rats. Korean J. Food Sci. Technol. 26: 213-220 (1994)
  8. Chung, H.S. and Ji, G.E. Composition and functionality of Chonma. Korean J. Food Sci. Technol. 26: 213-220 (1994)
  9. Reeves, P.G., Nielsen, F.H. and Fahey, G.C. AIN-93 purified diets for laboratory rodents. J. Nutr. 123: 1939-1951 (1993) https://doi.org/10.1093/jn/123.11.1939
  10. Jeong, M.K., Kim, M.H., Kang, N.E. and Kim, W.K. Effects of resistant starch on gut functions and plasma lipid profiles in rats fed high fat diet. J. Korean Soc. Food Sci. Nutr. 31: 271-276 (2002) https://doi.org/10.3746/jkfn.2002.31.2.271
  11. Friedewald, W.T., Levy, R.1. and Fredrickson, D.S. Estimation of LDL-cholesterol on plasma without use of the preparation ultracentrifuge. Clin. Chem. 18: 499-502 (1972)
  12. Snedecor, G.W. and Cochrane, W.G. Statistical Methods. 6th ed., p. 1. Iowa State University Press, Iowa, USA (1967)
  13. William, R.B., Robert, E. and Kathleen, N. Single-nutrient effects on immunologic functions. J. Am. Med. Assoc. 245: 53-58 (1981) https://doi.org/10.1001/jama.245.1.53
  14. Mertin, J., Meade, C.J. and Hunt, R. Importance of the spleen for the immuno-inhibitory action of linoleic acid in mice. Int. Archs. Allergy Appl. Immunol. 53: 469-473 (1977) https://doi.org/10.1159/000231785
  15. Lusis, A.J. Genetic factors affecting blood lipoprotein. J. Lipid Res. 29: 397-429 (1988)
  16. Green, M.S., Heiss, G., Rifkind, B.M., Cooper, G.R., Williams, O.D. and Tayroler, H.A. The ratio plasma high density lipoprotein cholesterol to total and low density lipoprotein cholesterol-age related changes and race and sex differences in selected North American populations. Circulation 72: 93-104 (1985) https://doi.org/10.1161/01.CIR.72.1.93
  17. Bae, J.C. Development of functional foods using chunma. Food Ind. Nutr. 7: 50-54 (2002)
  18. Grundy, S.M. and Denky, M.A. Dietary influences on serum lipids and lipoproteins. J. Lipid Res. 31: 1149-1172 (1990)
  19. Ebenbichler, C.F., Kichmair, R., Eagger, C. and Patch, J.R. Postprandial stage and atherosclerosis. Curr. Opinion Lipidol. 6: 286-290 (1995) https://doi.org/10.1097/00041433-199510000-00008
  20. Goldberg, I.J. Lipoprotein lipase and lipolysis: Central roles in lipoprotein metabolism and artherogenesis. J. Lipid Res. 37: 693-707 (1996)
  21. Oh, S.S. and Kaplan, M.L. Early treatment of obese(ob/ob) mice with triiodothyronine increases oxidative metabolism in muscle but not in brown adipose tissue or liver. J. Nutr. 125: 112-124 (1995)