Analysis of trans-Resveratrol Contents of Grape and Grape Products Consumed in Korea

포도와 포도 가공품에 함유되어 있는 trans-resveratrol의 함량 분석

  • Kim, Dae-Jung (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Kim, Sang-Kyun (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Kim, Myung-Hee (Korea Food Research Institute) ;
  • Lee, Hee-Bong (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee, Jun-Soo (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University)
  • 김대중 (충북대학교 식품공학과, 생물건강산업개발연구센터) ;
  • 김상균 (충북대학교 식품공학과, 생물건강산업개발연구센터) ;
  • 김명희 (한국식품개발연구원) ;
  • 이희봉 (충북대학교 식품공학과, 생물건강산업개발연구센터) ;
  • 이준수 (충북대학교 식품공학과, 생물건강산업개발연구센터)
  • Published : 2003.10.01


Resveratrol is natually occurring phytoalexin compounds produced by grape berries, peanuts, and their products in response to stress such as fungal infection, heavy metal ions or UV irradiation. The objective of this study was to develop a reliable high-performance liquid chromatographic method for the quantitative determination of trans-resveratrol in grape and its products. The trans-resveratrol was separated isocratically on Nucleosil 100-5 C18 column, using a mobile phase containing acetonitrile : water (40 : 60, v/v), detected by UV detector at 306 nm and the flow rate was 0.3 mL/min. Under this analytical condition, the recoveries of trans-resveratrol in grape, wine, and grape juice were 92.35, 104.72, and 91.08, respectively. Limit of detection in grape, wine, and grape juice were 14.5 ng/g, 3.62 ng/mL, and 4.02 ng/mL. Also, limit of quantitation in grape, wine, and grape juice were 14.8 ng/g, 3.69 ng/mL, and 4.10 ng/mL. Assay values of 32 grape varieties, 9 wines, and 9 grape juices were ranged from trace amount to $207.1\;{\mu}g/100\;g$, from 5.4 to $275.7\;{\mu}g/L$, and from 63.3 to $751.6\;{\mu}g/L$, respectively.


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