Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Chemical Structure of Polyphenol Isolated from Korean Pear (Pyrus pyrifolia Nakai)

한국산 배 (Pyrus pyrifolia Nakai)로부터 polyphenol 화합물의 구조결정

  • Zhang, Yun-Bin (Institute of Food Science and Technology, University of Shanghai) ;
  • Choi, Hee-Jin (Department of Food Science and Technology, Yeungnam University) ;
  • Han, Ho-Suk (Department of Food Science and Technology, Yeungnam University) ;
  • Park, Jung-Hye (Department of Food Science and Technology, Yeungnam University) ;
  • Son, Jun-Ho (Department of Food Science and Technology, Yeungnam University) ;
  • Bae, Jong-Ho (Department of Confectionery Decoration, Daegue Mirae College) ;
  • Seung, Tae-Su (Department of Food and Nutrition, Changwon Junior College) ;
  • An, Bong-Jeun (Faculty of Life Resource and Engineering, Kyungsan University) ;
  • Kim, Hyun-Gu (Korea Food Research Institute) ;
  • Choi, Cheong (Department of Food Science and Technology, Yeungnam University)
  • 장운빈 (중국 상하이대학교 식품공학연구소) ;
  • 최희진 (영남대학교 생물산업공학부) ;
  • 한호석 (영남대학교 생물산업공학부) ;
  • 박정혜 (영남대학교 생물산업공학부) ;
  • 손준호 (영남대학교 생물산업공학부) ;
  • 배종호 (대구 미래대학 제과 데코레이션과) ;
  • 성태수 (창원전문대학 식품영양과) ;
  • 안봉전 (경산대학교 생명자원공학부) ;
  • 김현구 (한국식품개발연구원) ;
  • 최청 (영남대학교 생물산업공학부)
  • Published : 2003.10.01
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Abstract

The polyphenol compounds of Korean pears were extracted with 60% acetone for 4 days at room temperature and purified using Sephadex LH-20 column chromatography, MCI gel column chromatography, Bondapak $C_{18}$ column chromatography, TLC, and HPLC. As a result, three compounds were isolated. The chemical structures of each compound were determined and identified using NMR, FAM-mass, and FT-IR. The compounds were confirmed as (+)-catechin (compound A), (+)-gallocatechin (compound B), (-)-epigallocatechin (compound C), and procyanidin B-3-3-o-gallate (compound D).

한국산 배를 60% acetone으로 추출하여 Sephadex LH-20 gel column chromatography, MCI-CHP 20 gel column chromatography, Bondapack $C_{18}$ gel column chromatography을 이용하여 TLC와 HPLC로 순도를 검증한 후 4종의 polyphenol 화합물을 분리, 정제하였다. Compound A와 B는 Sepadex LH-20 gel column chromatography에서 증류수상에서 용출되었고 compound C는 40% methanol상에서 용출됨을 보아 compound B와 compound C는 흡착성이 강한 polyphenol 화합물이라 추정되었다. 분리, 정제한 4종의 compound를 NMR, FAM-mass 및 FT-IR를 이용하여 화학구조를 결정한 결과 compound A는 (+)-catechin, compound B는 (+)-gallocatechin, compound C는 (-)-epigallocatechin이고 compound D는 procyanidin B-3-3-o-gallate로 밝혀졌다.

Keywords

References

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