Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Detection of Genetically Modified Maize Safety-approved in Korea Using PCR

PCR을 이용한 국내에서 안전성이 확인된 유전자재조합 옥수수의 분석 방법

  • Heo, Mun-Seok (College of Life Sciences, Kyung Hee University) ;
  • Kim, Jae-Hwan (College of Life Sciences, Kyung Hee University) ;
  • Park, Sun-Hee (Division of Food Microbiology, Korea Food and Drug Administration) ;
  • Woo, Geon-Jo (Division of Food Microbiology, Korea Food and Drug Administration) ;
  • Kim, Hae-Yeong (College of Life Sciences, Kyung Hee University)
  • 허문석 (경희대학교 생명과학대학 식품공학전공) ;
  • 김재환 (경희대학교 생명과학대학 식품공학전공) ;
  • 박선희 (식품의약품안전청 식품미생물과) ;
  • 우건조 (식품의약품안전청 식품미생물과) ;
  • 김해영 (경희대학교 생명과학대학 식품공학전공)
  • Published : 2003.12.01
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Abstract

Four lines (MON810, GA21, NK603, and TC1507)of genetically modified maize(GMM) were recently approved after a safety-assessment by the Korea Food and Drug Administration (KFDA). In this study, five pairs of specific oligonucleotide primers, based on the gene sequences inserted into maize and zein gene as internal standards, were designed and a method using PCR was developed for monitoring GMM and GMM derived foods circulating in the market. MON810 and GA21 were detected in raw materials of feed and food in the Korean market.

본 연구는 유전자재조합 기술에 의해 개발된 유전자재조합 옥수수(GMM)의 모니터링을 위하여 PCR을 이용한 검출방법에 대한 실험을 수행하였다. 최근 식품의약품안전청에서 안전성이 허가된 event MON810, GA2l, NK603, TC1507의 GMM에 삽입된 유전자와 표준대조 유전자인 zein의 유전자를 근거로 제작된 primer와 CTAB 방법으로 추출된 옥수수의 DNA를 template로 이용하여 PCR을 수행하였다. GMM의 검출을 위한, 제작된 primer들은 GMM와 특이적으로 반응하여 증폭된 PCR 산물을 생성하였다. 국내시장에 유통 중인 식용과 사료용의 원료옥수수에서 모니터링 하여 이러한 유전자재조합 옥수수의 일부가 국내에 유통 중인 것을 확인할 수 있었다.

Keywords

References

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