Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Analysis of Radiolytic Products of Lipid for the Detection of Irradiated Dried Cuttle Fish (Sepia officinalis)

건 갑오징어의 방사선 조사여부를 판별하기 위한 지방분해산물 분석

  • 김준형 (조선대학교 식품영양학과) ;
  • 김경수 (조선대학교 식품영양학과)
  • Published : 2003.12.01
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Abstract

Radiation-induced hydrocarbons and 2-alkylcycolbutanones are formed from the fatty acids of irradiated fat. These radiation-induced compunds were detected by fat extraction with a Soxtec apparatus from dried cuttle fish (Sepia officinalis), isolation of hydrocarbons and 2-alkylcyclobutanones with florisil column chromatography, and identification of GC/MS. Concentration of hydrocarbons produced by -λ-irradiation depended on the composition of fatty acid in dried cuttle fish. The major hydrocarbons in the irradiated dried cuttle fish samples were pentadecane and 1-tetradecene from palmitic acid, heptadecane and 1-hexadecene from stearic acid, and 8-heptadecen and 1,7-hexadecadiene from oleic acid. Of 2-alkylcyclobutanones, 2-dodecylcyclobutanone from palmitic acid was present at the highest level in irradiated dried cuttle fish. The radiation-induced hydrocarbons and 2-alkylcyclobutanones from the irradiated dried cuttle fish were detected at 0.5 kGy and over, but not detected in the non-irradiated fish.

방사선 조사된 건갑오징어로부터 유도된 hydrocarbon류와 2-alkylcyclobutanone류의 양을 비교 분석하였다. 건갑오징어의 지방추출은 Soxtec 기기를 이용하였으며, 지방추출 후 florisil 충진한 column을 이용하여 hydrocarbon류와 2-alkylcyclobutanone류를 분리하고 GC/MS 분석기기로 확인하였다. 0.5kGy부터 10kGy까지의 방사선 선량별로 조사된 각 시료에서 생성된 hydrocarbon류는 0.5kGy 이상 조사된 시료에서 검출되었으며 조사선량에 따라 생성량도 증가하였다. Palmitic acid와 stearic acid로부터 생성된 pentadecane과 1-tetradecene 그리고 heptadecane과 1-hexadecene의 함량이 가장 많았으며. 함량이 낮은 oleic acid로부터 생성된 8-heptadecene과 1,7-hexadecadiene은 가장 적은 함량으로 확인되었다. 2-Alkylcyclobutanone류는 0.5kGy 이상 조사된 시료에서 검출되었으며, palmitic acid로부터 생성된 2-dodecylcyclo-butanone이 두 시료 모두 가장 많이 생성되었다. Stearic acid로부터 생성된 2-tetradecylcyclobutanone 역시 조사선량과 지방산 함량에 따라 생성량이 증가되는 경향을 보였으나 그 함량이 너무 낮게 검출되었다. 본 연구 결과, 방사선 조사된 건오징어와 건갑오징로부터 생성된 hydrocarbon류는 조사선량에 따라 뚜렷하게 검출되었고 비조사 시료와 비교, 확인이 가능하여 방사선 조사 여부 판별뿐만 아니라 흡수선량을 예측하기 위한 marker로서 활용할 수 있으며, 2-alkylcyclobutanone류는 방사선 조사 유무의 marker로서 가능성을 확인하였다.

Keywords

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