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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Isolation and Identification of an Antioxidant Enzyme Catalase Stimulatory Compound from Garnoderma lucidum

  • Lee, Hyeon-Yong (Division of Food and Biotechnology, Kangwon National University) ;
  • Eum, Won-Sik (Division of Life Sciences, Hallym University) ;
  • Kim, Dae-Won (Division of Life Sciences, Hallym University) ;
  • Lee, Byung-Ryong (Division of Life Sciences, Hallym University) ;
  • Yoon, Chang-Sik (Division of Life Sciences, Hallym University) ;
  • Jang, Sang-Ho (Division of Life Sciences, Hallym University) ;
  • Choi, Hee-Soon (Division of Life Sciences, Hallym University) ;
  • Choi, Soo-Hyun (Division of Life Sciences, Hallym University) ;
  • Baek, Nam-In (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Kang, Jung-Hoon (Department of Genetic Engineering, Chongju University) ;
  • Kang, Tae-Cheon (Department of Anatomy, College of Medicine, Hallym University) ;
  • Won, Moo-Ho (Department of Anatomy, College of Medicine, Hallym University) ;
  • Cho, Sung-Woo (Department of Biochemistry, University of Ulsan College of Medicine) ;
  • Lee, Kil-Soo (Division of Life Sciences, Hallym University) ;
  • Park, Jin-Seu (Division of Life Sciences, Hallym University) ;
  • Choi, Soo-Young (Division of Life Sciences, Hallym University)
  • Received : 2003.02.27
  • Accepted : 2003.03.12
  • Published : 2003.09.30
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Abstract

Antioxidant enzymes are scavenger reactive-oxygen intermediates and are involved in many cellular defense systems. We previously reported that a crude extract of Garnoderma lucidum, a medicinally potent mushroom, profoundly increased the catalase gene expression and enzyme activities in mouse livers (Park et al., J. Biochem. Mol. Biol. 34. 144-149, 2001). In this study, we elucidated the detailed mechanism whereby G. lucidum stimulates the catalase activity and expression. The major active fraction was isolated from G. lucidum and methyl linoleate was considered the most major component of the fraction. In order to determine whether methyl linoleate increases mRNA and protein synthesis of catalase, Northern and Western blot analyses were performed in vivo with methyl linoleate-treated mouse liver homogenate after feeding methyl linoleate to the mice. Northern and Western blot analyses of the crude liver homogenates in the mice that were administered methyl linoleate revealed that the expression catalase was significantly increased when compared to the untreated controls. In addition, the catalase protein levels and enzymatic activities increased in the mouse liver homogenates. These results suggest that methyl linoleate that is produced by G. lucidum stimulates the catalase expression at the transcription level.

Keywords

References

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