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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Molecular Gene Cloning, Expression, and Characterization of Bovine Brain Glutamate Dehydrogenase

  • Kim, Dae-Won (Department of Genetic Engineering, Division of Life Sciences, Hallym University) ;
  • Eum, Won-Sik (Department of Genetic Engineering, Division of Life Sciences, Hallym University) ;
  • Jang, Sang-Ho (Department of Genetic Engineering, Division of Life Sciences, Hallym University) ;
  • Yoon, Chang-Sik (Department of Genetic Engineering, Division of Life Sciences, Hallym University) ;
  • Kim, Young-Hoon (Department of Genetic Engineering, Division of Life Sciences, Hallym University) ;
  • Choi, Soo-Hyun (Department of Genetic Engineering, Division of Life Sciences, Hallym University) ;
  • Choi, Hee-Soon (Department of Genetic Engineering, Division of Life Sciences, Hallym University) ;
  • Kim, So-Young (Department of Genetic Engineering, Division of Life Sciences, Hallym University) ;
  • Kwon, Hyeok-Yil (Department of Physiology, College of Medicine, Hallym University) ;
  • Kang, Jung-Hoon (Department of Genetic Engineering, Chongju University) ;
  • Kwon, Oh-Shin (Department of Biochemistry, College of Natural Science, Kyungpook National University) ;
  • Cho, Sung-Woo (Department of Biochemistry, University of Ulsan College of Medicine) ;
  • Park, Jin-Seu (Department of Genetic Engineering, Division of Life Sciences, Hallym University) ;
  • Choi, Soo-Young (Department of Genetic Engineering, Division of Life Sciences, Hallym University)
  • Received : 2003.05.03
  • Accepted : 2003.06.02
  • Published : 2003.11.30
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Abstract

A cDNA of bovine brain glutamate dehydrogenase (GDH) was isolated from a cDNA library by recombinant PCR. The isolated cDNA has an open-reading frame of 1677 nucleotides, which codes for 559 amino acids. The expression of the recombinant bovine brain GDH enzyme was achieved in E. coli. BL21 (DE3) by using the pET-15b expression vector containing a T7 promoter. The recombinant GDH protein was also purified and characterized. The amino acid sequence was found 90% homologous to the human GDH. The molecular mass of the expressed GDH enzyme was estimated as 50 kDa by SDS-PAGE and Western blot using monoclonal antibodies against bovine brain GDH. The kinetic parameters of the expressed recombinant GDH enzymes were quite similar to those of the purified bovine brain GDH. The $K_m$ and $V_{max}$ values for $NAD^+$ were 0.1 mM and $1.08\;{\mu}mol/min/mg$, respectively. The catalytic activities of the recombinant GDH enzymes were inhibited by ATP in a concentration-dependent manner over the range of 10 - $100\;{\mu}M$, whereas, ADP increased the enzyme activity up to 2.3-fold. These results indicate that the recombinant-expressed bovine brain GDH that is produced has biochemical properties that are very similar to those of the purified GDH enzyme.

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References

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