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Differential Responses of Rice Acid Phosphatase Activities and Isoforms to Phosphorus Deprivation

  • Lim, Jeong-Hyun (Institute for Agricultural Science and Technology, Chonbuk National University) ;
  • Chung, Ill-Min (Research Team of Friendly Environmental Low-Input Natural Herbicide New Material Study, Konkuk University) ;
  • Ryu, Sang-Soo (Institute for Agricultural Science and Technology, Chonbuk National University) ;
  • Park, Myoung-Ryoul (Institute for Agricultural Science and Technology, Chonbuk National University) ;
  • Yun, Song-Joong (Institute for Agricultural Science and Technology, Chonbuk National University)
  • Received : 2003.04.19
  • Accepted : 2003.06.12
  • Published : 2003.11.30
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Abstract

Acid phosphatases (APases) play a role in the release of phosphate in organic complexes in soil. We investigated tissue- and isoform-specific responses of APases to phosphorus (P) deficiency in three rice genotypes; Dasan-byeo, Sobi-byeo, and Palawan. The levels of shoot APase activity per protein were similar in the three genotypes. They significantly decreased with P deprivation that was longer than seven days. Root APase activity per protein was two- to three-fold higher in Dasan than in Sobi and Palawan. In all genotypes the APase activity increased in P-deficient plants, but the increase was higher in Sobi and Palawan. After 21 days of P deprivation, secreted APase activity increased more than eight-fold in Dasan and two-fold in Sobi and Palawan. Isoform profiles of shoot and root APases were most diverse in Dasan. The activities of the major isoforms in P-deficient shoots decreased in all three genotypes. Depending on the genotypes, further increases in constitutive isoforms and new induction of one to four isoforms occurred in P-deficient roots. The results indicate that tissue and genotype differences in the response of APase to P deficiency are primarily facilitated by the different responses of the isoforms.

Keywords

References

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