DOI QR코드

DOI QR Code

Effects of Growth Factors and Gut Regulatory Peptides on Glucose Uptake in HC 11 Mouse Mammary Epithelial Cells

  • Myung, K.H. (Department of Animal Science, Chonnam National University)
  • 투고 : 2003.05.29
  • 심사 : 2003.07.16
  • 발행 : 2003.11.01

초록

The large and rapid changes of glucose utilization in lactating mammary tissue in response to changes in nutritional state must be largely related by external signal of insulin. This also must be related with the quantity and composition of the diet in vivo. To characterize the mode of growth factors and gut regulatory peptides with insulin, in vitro experiment was conducted with HC11 cells. All the growth factor alone and the combinations of growth factors significantly (p<0.05) increased in glucose uptake. Insulin, EGF and IGF-1 exhibited a stimulation of glucose uptake for at least 24 h. Furthermore, the highest (p<0.05) synergistic effect was shown in EGF plus IGF-1 and the second synergistic effect in insulin plus EGF while no synergistic effect was found between insulin and IGF-1. However, the gut regulatory peptides neither potentiated nor inhibited the action of insulin on glucose uptake. Although growth factors did not modulates glucose uptake via increasing the rate of translation of the GLUT1 protein, RT-PCR analysis indicated that the growth factors significantly (p<0.05) increased the expression of GLUT1. The growth factors are therefore shown to be capable of modulating glucose uptake by transcription level with insulin in HC 11 cells.

키워드

참고문헌

  1. Bennett, B. L. 1995. Glucose transporter gene expression in mammary tissue. Ph. D. Thesis, Univ.of Otago, New Zealand.
  2. Bernier, M. D., M. Laird and M. D. Lane. 1988. Effects of vanadate on the cellular accumulation of pp15, an apparent product of insulin receptor tyrosine kinase action. J. Biol. Chem. 263:13626-13634.
  3. Cesarone, C. F., C. Bolognesi and M. Peaker. 1980. Improved microflurorometric DNA determination n biological material using 33258 Hoescht. Anal. Boichem. 100:188-197.
  4. Choi, B. H., K. W. Stewart, S. R. Davis and K. H. Myung. 2002. Bioluminescent determination of lactose secretion: A Measures of the in vitro performance of mammary acini from lactating rats. Asian-Aust. J. Anim. Sci. 15:274-278.
  5. Chomozynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-choloform extraction. Anal. Biochem. 162:156-159.
  6. Doppler, W., B. Groner and R. K. Ball. 1989. Prolactin and glucocorticoid hormones synergistically induce expression of transfected rat beta-casein gene promoter constructs in a mammary epithelial ell line. Proc. Natl. Acad. Sci. USA. 86:104-108.
  7. Faulkner, A. and M. Peaker. 1987. Regulation of mammary glucose metabolism in lactation. New York, Plenum Press.
  8. Laemmli, U. K. 1970. Cleveage of structural proteins during the assembled of the head of bacteriophage T4. Nature. 227:680-685. https://doi.org/10.1038/227680a0
  9. Linzell, J. L. 1967. The effect of very frequent milking and of oxytocin on the yield and composition of milk in fed and fasted goats. J. Physiol. 190:333-346.
  10. Mercer, S. W. and D. H. Williamson. 1986. Time course of changes in plasma glucose and insulin concentrations and mammary gland lipogenesis during refeeding of starved conscious lactating rats. Biochem. J. 239:489-492.
  11. Merlo, G. R., D. Graus-Porta, N. Cella, B. M. Marte, D. Taverna and N. E. Hynes. 1996. Growth, differentiation and survival of HC11 mammary epitherial cells: diverse effects of receptor tyrosine kinase-activationg peptide growth factors. European J. Cell Biol. 70:97-105.
  12. Merlo, G. R., L. Fiore, F. Basolo, L. Duboc and N. E. Hynes. 1995. P53 dependent and p53 independent apoptosis in mammary epitherial cells reveals a role for EGF and insulin as survival factors. J. Cell Biol. 128:1185-1196.
  13. Page, T. 1989. Evidence for the involvement of a gastrointestinal peptide in the regulation of glucose uptake in the mammary gland of the lactating rat. Biochem. J. 258:39-643.
  14. Roe, J. A. A. S. Haji Bara, J. M. M. Harper and P. J. Buttery. 1995. Effects of growth factors and gut regulatory peptides on nutrient uptake in ovine muscle cell cultures. Comp. Biochem. Physiol. 110A:107-114.
  15. SAS Institute Inc. 1997. SAS Users Guide: Release 6.07. Cary, North Carolina, SAS Institute.
  16. Threadgold, L. C. and N. J. Kuhn. 1984. Monosaccharide transport in the mammary gland of intact lactating rat. Biochem. J. 218:213-219.
  17. Usdin, T. B., E. Mezey, D. C. Button, M. J. Brownstein and T. I. Bonner. 1993. Gastric inhibitory polypeptide receptor, a member of the secretin-vasoactive intestinal peptide receptor family, is widely distributed in peripheral organs and the brain. Endocrinology. 133:2861-2870.
  18. Wang, Y., H. K. Kole, C. Montrose-Rafizadeh, R. Perfetti, M. Bernier and J. M. Egan. 1997. regulation of glucose transporters and hexose uptake in 3T3-L1 adipocyte: glucogan-like peptide-1 and insulin interactions. J. Mol. Endocrinol. 19:241-248.
  19. William, I. S. and E. C. Lee. 1991. Assessment of glucose transporter gene expression using polymerase chain reaction. Endocrinology, 128:2387-2394.