Asian-Australasian Journal of Animal Sciences

  • 제16권8호
  • /
  • Pages.1151-1157
  • /
  • 2003
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Influences of Surfactant Tween 80 on the Gas Production, Cellulose Digestion and Enzyme Activities by Mixed Rumen Microorganisms

  • Lee, Sung S. (Division of Animal Science and Technology, Gyeongsang National University) ;
  • Ha, Jong K. (Division of Animal Science and Technology, Gyeongsang National University)
  • 투고 : 2003.01.17
  • 심사 : 2003.04.17
  • 발행 : 2003.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The surfactant Tween 80 was evaluated for its ability to influence cumulative gas production, cellulose digestion, and enzyme activities by mixed ruminal microorganisms grown on barley grain or Orchardgrass hay. The addition of Tween 80 at a level of 0.10% significantly (p<0.05) decreased the cumulative gas production rate from both barley grain or Orchardgrass hay substrates. However, 0.05% Tween 80 did not affect gas production rates compared to the control treatment. The addition of 0.05% Tween 80 to cultures growing on barley grain resulted in a significant increase in cellulase (90.01%), xylanase (90.73%) and amylase (487.25%) activities after 30 h incubation. Cultures utilizing Orchardgrass hay had a significant increase in cellulase (124.43%), xylanase (108.86%) and amylase (271.22%) activities after 72 h incubation. These increases in activities were also observed with cultures supplemented with 0.10% Tween 80 throughout all the incubation times tested. These results indicated that the addition of 0.05% Tween 80 could greatly stimulate the release of some of key enzymes without decreasing cell growth rate in contrast to trends reported with aerobic microorganism. Our data indicates potential uses of the surfactant Tween 80 as a feed additive for ruminant animals.

키워드

참고문헌

  1. Bryant, M. P. and L. A. Burkey. 1953. Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J. Dairy Sci. 36:205-217. https://doi.org/10.3168/jds.S0022-0302(53)91482-9
  2. Demain, A. L. and J. Birnbaum. 1968. Alternation of permeability for the release of metabolites from microbial cells. Curr. Topics Microbiol. Immun. 46:1-25.
  3. Deshpande, M. V., M. C. Srinavasan and S. S. Deshmakh. 1987. Effects of fatty acids on cellulase production by Penicillium funiculosum and its mutants. Biotechnol. Lett. 9:301-304. https://doi.org/10.1007/BF01025792
  4. Fedorak, P. M. and S. E. Hrwdey. 1983. A simple apparatus for measuring gas production by methanogenic cultures in serum bottles. Environ. Technol. Lett. 4:425-432. https://doi.org/10.1080/09593338309384228
  5. Ha, J. K., S. S. Lee, Z. Gao, C. -H Kim, S. W. Kim and K. -J. Cheng. 2001. The effect of saturated fatty acids on cellulose digestion by rumen anaerobic fungus, Neocallimastix frontalis C5-1. Asian-Aust. J. Anim. Sci. 14:941-946.
  6. Herrero, M., I. Murray, R. H. Fawcett and J. B. Dent. 1996. Prediction of the in vitro gas production and chemical composition of kikuyu grass by near-infrared reflectance spectroscopy. Anim. Feed Sci. Technol. 60:51-67. https://doi.org/10.1016/0377-8401(95)00924-8
  7. Hulme, M. A. and D. W. Stranks. 1970. Induction and their regulation of production of cellulase by fungi. Nature, London. 226:469-470. https://doi.org/10.1038/226469a0
  8. Hungate, R. E. 1950. The anaerobic mesophyllic cellulolytic bacteria. Bacteriol. Rev. 14:1-49.
  9. Hung, B. R., L. Lara, M. A. Patron, N. N. Ugarova, W. Bechstedt, and S. Clappes. 1988. Tween 80 and proteose peptone effect on cellulase production. Acta Biotechnol. 8:461-464. https://doi.org/10.1002/abio.370080515
  10. Lee, S. S., J. K. Ha and K. -J. Cheng. 2001. Effects of LCFA on the gas production, cellulose digestion and celluase activities by rumen anaerobic fungus, Neocallimastix frontalis RE1. Asian-Aust. J. Anim. Sci. 14:1110-1117.
  11. Lee, S. S., C. H. Kim, J. K. Ha, Y. H. Moon, N. J. Choi and K. J. Cheng. 2002. Distribution and activities of hydrolytic enzymes in the rumen compartments of Hereford bulls fed alfalfa based diet. Asian-Aust. J. Anim. Sci. Vol 15: 1725-1731.
  12. Lee, S. S., B. H. Ahn, H. S. Kim, C. H. Kim, K. -J. Cheng and J. K. Ha. 2003. Effects of non-ionic surfactants on enzyme distributions of rumen contents, anaerobic growth of rumen microbes, rumen fermentation characteristics and performances of lactating cows. Asian-Aust. J. Anim. Sci. 16:104-115.
  13. Long, K. and J. S. Knapp. 1991. The effect of Junlon PW110 and Tween 80 on the production of cellulolytic enzymes by Coprinus cinereus. Mycol. Res. 95:1077-1081. https://doi.org/10.1016/S0953-7562(09)80550-9
  14. Lowe, S. E., G. G. Griffith, A. Milne, M. K. Theodorou and A. P. J. Trinci. 1987a. The life cycle and growth kinetics of an anaerobic rumen fungus. J. Gen. Microbiol. 133:1815-1827.
  15. Lowe, S. E., M. K. Theodorou and A. P. J. Trinci. 1987b. Isolation of anaerobic fungi from saliva and faeces of sheep. J. Gen. Microbiol. 133:1829-1834.
  16. Merry, R. J., M. K. Theodorou, M. G. Raurich and M. S. Dhanoa. 1991. Use of head-space gas pressure in batch cultures to assist in determination of nutritive value of silage to rumen bacteria. Presented at a Conference on Forage Conservation Towards 2000. Institute of Grassland and Forage Research, Federal Research Centre of Agriculture, Brauschweig-Volkenrode, Germany.
  17. Miller, G. L. 1959. Use of dinitrosalycilic acid reagent for determination of reducing sugar. Analytical Chemistry 31:426-428. https://doi.org/10.1021/ac60147a030
  18. Miller, T. L. and M. J. Wolin. 1974. A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl. Microbiol. 27:985-987.
  19. Mountfort, D. O. and R. A. Asher. 1983. Role of catabolite regulatory mechanisms in control of carbohydrate utilization by the rumen anaerobic fungus Neocallimastix frontalis. Appl. Environ. Microbiol. 46:1331-1338.
  20. Reese, E. T. and A. Maguire. 1969. Surfactants as stimulants of enzyme production by microorganisms. Applied Microbiol. 17:242-245.
  21. SAS. 1996. Statistical analysis systems user's guide: Statistics. SAS Institute. Inc., Cary, NC.
  22. Schewale, J. G. and J. C. Sadana. 1978. Cellulase and -glucosidase production by basidiomycetes species. Can. J. Microbiol. 24:1204-1216. https://doi.org/10.1139/m78-195
  23. Theodorou, M. K., D. R. David, B. B. Nielsen, M. I. G. Lawrence, and A. P. J. Trinci. 1995. Determination of growth of anaerobic fungi on soluble and cellulosic substrates using a pressure transducer. Microbiology 141:671-678. https://doi.org/10.1099/13500872-141-3-671
  24. Yazdi, M. T., J. R. Woodward, and A. Radford. 1990. The cellulase complex of Neurospora crassa: activity, stability and release. J. Gen. Microbiol. 136:1313-1319. https://doi.org/10.1099/00221287-136-7-1313

피인용 문헌

  1. Short communication: Effects of lysolecithin on milk fat synthesis and milk fatty acid profile of cows fed diets differing in fiber and unsaturated fatty acid concentration vol.100, pp.11, 2017, https://doi.org/10.3168/jds.2017-12976
  2. Effects of dietary supplementation with alkyl polyglycoside, a nonionic surfactant, on nutrient digestion and ruminal fermentation in goats1 vol.88, pp.12, 2010, https://doi.org/10.2527/jas.2009-2397
  3. Effect of sodium stearoyl-2-lactylate supplementation on lactation performance, blood-biochemical profile, and economic efficacy of mid-lactation Holstein cows vol.31, pp.9, 2018, https://doi.org/10.5713/ajas.18.0367
  4. Effect of optimal sodium stearoyl-2-lactylate supplementation on growth performance and blood and carcass characteristics in Hanwoo steers during the early fattening period vol.31, pp.9, 2018, https://doi.org/10.5713/ajas.18.0349
  5. Effects of Tween 80 Pretreatment on Dry Matter Disappearance of Rice Straw and Cellulolytic Bacterial Adhesion vol.20, pp.9, 2003, https://doi.org/10.5713/ajas.2007.1397
  6. 양(+) 이온성 및 음(-) 이온성 계면활성제 첨가가 반추위 혼합 미생물에 의한 In vitro 건물소화율 및 미생물 성장에 미치는 영향 vol.49, pp.5, 2007, https://doi.org/10.5187/jast.2007.49.5.647
  7. 비이온성 계면활성제 Tween 80의 첨가가 반추위 혼합 미생물에 의한 in vitro 가스발생량, 건물소화율, 효소활력 및 미생물 성장율에 미치는 영향 vol.17, pp.12, 2003, https://doi.org/10.5352/jls.2007.17.12.1660
  8. Effects of Mixtures of Tween80 and Cellulolytic Enzymes on Nutrient Digestion and Cellulolytic Bacterial Adhesion vol.21, pp.11, 2003, https://doi.org/10.5713/ajas.2008.80333
  9. 비이온성 및 양쪽 이온성 계면활성제 첨가가 반추위 혼합 미생물의 성장과 볏짚의 in vitro 소화에 미치는 영향 vol.18, pp.4, 2008, https://doi.org/10.5352/jls.2008.18.4.515
  10. Effects of Non-ionic Surfactant Supplementation on Ruminal Fermentation, Nutrient Digestibility and Performance of Beef Steers Fed High-roughage Diets vol.22, pp.7, 2003, https://doi.org/10.5713/ajas.2009.90095
  11. Effect of essential oil blends and a nonionic surfactant on rumen fermentation, anti-oxidative status, and growth performance of lambs vol.5, pp.3, 2003, https://doi.org/10.1093/tas/txab118