Asian-Australasian Journal of Animal Sciences

  • 제16권12호
  • /
  • Pages.1716-1721
  • /
  • 2003
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Co-expression and Sequence Determination of Estrogen Receptor Variant Messenger RNAs in Swine Uterus

  • Ying, C. (Department of Microbiology, Soochow University) ;
  • Chan, M.-A. (Department of Microbiology, Soochow University) ;
  • Cheng, W.T.K. (Department of Animal Sciences, National Taiwan University) ;
  • Hong, W.-F. (Department of Microbiology, Soochow University)
  • 투고 : 2003.01.14
  • 심사 : 2003.05.20
  • 발행 : 2003.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Steroid hormones and their receptors play an important role in reproductive process. Estrogen is intimately involved with pregnancy and its function is mediated through the estrogen receptor which has been chosen as a candidate gene to study litter size in pigs. In this study, we report that two estrogen receptor variants, designated pER-1 and pER-2 were co-expressed in the uteri of normal cycling Lan-Yu pig (Sus vittatus; a small-ear miniature in Taiwan) with the pER-1 expression level appeared to be several times higher than that of pER-2. These receptor variants were isolated using reverse transcription-PCR from the pig uteri and their sequences were determined. The pER-1 and pER-2 sequences, which are homologous to those found in other mammalian estrogen receptors, encode putative proteins consisting of 574 and 486 amino acids, respectively. A deletion in exon I was identified in both sequences, with deletion lengths of 63 bp in pER-1 and 327 bp in pER-2. The deletion in pER-1 is internal to that in pER-2 and both deletions resulted in a truncation of the B domain, which confers the transactivating activity of estrogen receptor protein. This result describes the existence of estrogen receptor variants with a deletion in exon I and implies the possibility that physiological functioning of an estrogen receptor may not require the presence of an intact B domain.

키워드

참고문헌

  1. Bokenkamp, D., P. W. Jungblut, H. H. Thole. 1994. The Cterminal half of the porcine estradiol receptor contains no posttranslational odification:determination of the primary structure. Mol. Cell Endocrinol. 104:163-172.
  2. Chomczynski, P., N. Sacchi. 1987. Single-step method of RNA isolated by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
  3. Cooke, P. S., D. L. Buchanan, D. B. Lubahn, G. R. Cunha. 1998. Mechanism of estrogen action: Lessons from the estrogen receptor-alpha knockout mouse. Biol. Reprod. 59:470-475.
  4. Couse, J. F., J. Lindzey, K. Grandien, J. A. Gustafsson. 1997. Tissue destruction and quantitative analysis of estrogen receptor-(ER) and estrogen receptor-(ER) messenger ribonucleic acid in the wild-type and ER- knockout mouse. Endocrinology, 138:4613-4621.
  5. Das, S. K., J. A. Taylor, K. S. Korach, B. C. Paria, S. K. Dey, D. B. Lubahn. 1997. Estrogenic responses in estrogen receptor-alpha deficient mice reveal a distinct estrogen signaling pathway. Proc. Natl. Acad. Sci. USA. 94:12786-12791.
  6. Diaz-Chico, B. N., Y. Ogasawara, G. C. Chamenss, M. Salman, W. L. McGuire. 1988. A 46-kDa antigen associated with estrogen receptor in human breast cancer. J. Steroid. Biochem. 30:315-320.
  7. Evans, R. M. 1988. The steroid and thyroid hormone receptor superfamily. Science, 240:889-895. https://doi.org/10.1126/science.3283939
  8. Green, S., P. Walter, V. Kumar, A. Krust, J-M. Bornert, P. Argos, P. Chambon. 1986. Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A. Nature (Lond.) 320:134-139.
  9. Gotteland, M., G. Desauty, J. C. Delarue, L. Liu and E. May. 1995. Human estrogen receptor messenger RNA variants in both normal and tumor breast tissues. Mol. Cell Endocrinol. 112:1-13.
  10. Graham, M. L., N. L. Krett, L. A. Miller, K. K. Leslie, D. F. Gordon, W. M. Wood, L. L. Wei and K. B. Horwitz. 1990. T47Dco cells, genetically unstable and containing estrogen receptor mutations, are a model for the progression of breast cancers to hormone resistance. Cancer Res. 50:6208-6217
  11. Gronemeyer, H. 1991. Transcription activation by estrogen and progesterone receptors. Ann. Rev. Gen. 25:89-123.
  12. Jozan, S., A. M. Julia, A. Carretie, N. Eche, V. Maisongrosse, B. Fouet, B. Marques and J. F. David. 1991. 65 and 47 kDa forms of estrogen receptor in human breast cancer: relation with estrogen responsiveness. Breast Cancer Res. Treat. 19:103-109.
  13. King, R. J. B. 1991. A discussion of the roles of oestrogen and progestin in human mammary carcinogenesis. J. Steroid Biochem Mol Biol. 39:811-818.
  14. Koehorst, S. G. A., H. M. Jacobs, J. H. H. Thijssen and M. A. Blankenstein. 1993. Wild type and alternatively spliced estrogen receptor messenger RNA in human meningioma tissue and MCF7 breast cancer cells. J. Steroid. Biochem. Mol. Biol. 45:227-233.
  15. Koike, S., M. Sakai and M. Muramatsu. 1987. Molecular cloning and characterization of rat estrogen receptor cDNA. Nuc Acids Res. 15:2499-2513. https://doi.org/10.1093/nar/15.6.2499
  16. Krege, J. H., J. B. Hodgin, J. F. Couse, E. Enmark, M. Warner, J. F. Mahler, M. Sar, K. S. Korach, J. A. Gustafsson and O. Smithies. 1998. Generation and reproductive phenotypes of mice lacking estrogen receptor beta. Proc. Natl. Acad. Sci. USA. 95:15677-15682.
  17. Kuiper, G. G. M. J., E. Enmark, M. Pelto-Huikko, S. Nilsson and J. A. Gustafsson. 1996. Cloning of a novel estrogen receptor expressed in rat prostate and ovary. Proc. Natl. Acad Sci. USA. 93:5925-5930.
  18. Kumar, V., S. Green, G. Stack, M. Berry, J-R. Jin and P. Chambon 1987. Functional domains of the human estrogen receptor. Cell, 51: 941-951.
  19. Kuiper, G. G. J. M., B. Carlsson, K. Grandien, E. Enmark, J. Haggblad, S. Nilsson and J-A. Gustafsson. 1997. Comparison of ligand binding specificity and transcript tissue distrubution of estrogen receptors and Endocrinology, 138:863-870.
  20. Lahooti, H., R. White, P. Danielian and M. G. Parker. 1994. Characterization of ligand-dependent phosphorylation of the estrogen receptor. Mol. Endocrinol. 8:182-188.
  21. LeGoff, P., M. M. Montano, D. J. Schodin, B. S. Katzenellenbogen. 1994. Phosphorylation of the human estrogen receptor: identification of hormone-regulated sites and examination of their influence on transcriptional activity. J. Biol. Chem. 269:4458-4466.
  22. Madigou, T., C. Tiffoche, G. Lazennec, J. Pelletier and M. L. Thieulant. 1996. The sheep estrogen receptor: Cloning and regulation of expression in the hypothalamo-pituitary axis. Mol. Cell Endocrinol. 121:153-163.
  23. Mason, I. L. 1996. A world dictionary of livestock breeds, types and varieties, CAB International, Rome, Italy.
  24. McGuire, W. L., G. C. Chamness and S. A. W. Fuqua. 1992. Abnormal estrogen receptor in clinical breast cancer. J. Steroid. Biochem. Mol. Biol. 43:243-247.
  25. Moffatt, C. A., E. F. Rissman, M. A. Shupnik and J. D. Blaustein. 1998. Induction of progestin receptors by estradiol in the forebrain of estrogen receptor-alpha gene-disrupted mice. Neuroscience, 18:9556-9563.
  26. Murdoch, F., D. Meier, D. Furlow, K. Grunwald and J. Gorski. 1990. Estrogen receptor binding to a DNA response element in vitro is not dependent upon estradiol. Biochemistry 29:8377-8385.
  27. Pfeffer, U., E. Fecarotta, L. Castagnetta and G. Vidali 1993. Estrogen receptor variant messenger RNA lacking exon 4 in estrogen-responsive human breast cancer cell lines. Cancer Res. 53:41-743.
  28. Pfeffer, U., E. Fecarotta and G. Vidali. 1995. Coexpression of multiple estrogen receptor variant messenger RNAs in normal and neoplastic breast tissues and in MCF-7 cells. Cancer Res. 55:21582165.
  29. Pink, J. J., S. Q. Wu, D. M. Wolf, M. M. Bilimoria and V. C. Ordan. 1996. A novel 80 kDa human estrogen receptor containing a duplication of exons 6 and 7. Nucl. Acids Res. 24:962-969.
  30. Rissman, E. F., S. R. Wersinger, J. A. Taylor and D. B. Lubahn. 1997. Estrogen receptor function as revealed by knockout studies: Neuroendocine and behavioral aspects. Hormone Behav. 31:232-243.
  31. Rothschild, M., C. Jacobson, D. Vaske, C. Tuggle, L. Wang, T. Short, G. Eckardt, S. Sasaki, A. Vincent, D. McLaren, O. Southwood, H. Van Der Steen, A. Mileham, G. Plastow. 1996. The estrogen receptor locus is associated with a major gene influencing litter size in pigs. Proc. Natl. Acad. Sci. USA. 93:201-205.
  32. Schomberg, D. W., J. F. Course, A. Mukherjee, D. B. Lubahn, M. Sar, K. E. Mayo and K. S. Korach. 1999. Targeted disruption of the estrogen receptor-alpha gene in female mice: characterization of ovarian responses and phenotype in the adult. Endocrinology 140:2733-2744.
  33. Saunders, P. T. K. L., S. M. Maguire, J. Gaughan and M. R. Millar. 1997. Expression of oestrogen receptor beta in multiple rat tissues visualized by immunohistochemistry. J. Endocrinol. 154:13-16. https://doi.org/10.1677/joe.0.154R013
  34. Tremblay, G. B., A. Tremblay, N. G. Copeland, D. J. Gilbert, N. A. Jenkins, F. Labrie and V. Giguere. 1997. Cloning, chromosomal localization and functional analysis of the murine estrogen receptor beta. Mol. Endocrinol. 11:353-365.
  35. Wang, Y., R. J. Miksicek. 1991. Identification of a dominant negative form of the human estrogen receptor. Mol. Endocrinol. 5:1707-1715.

피인용 문헌

  1. Effects of Dietary Algal Docosahexaenoic Acid Oil Supplementation on Fatty Acid Deposition and Gene Expression in Laying Tsaiya Ducks vol.19, pp.7, 2003, https://doi.org/10.5713/ajas.2006.1047