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Purification and Characterization of Complement-activating Acidic Polysaccharides from the Fruits of Capsicum annuum

  • Paik, Soon-Young (Department of Microbiology, College of Medicine, The Catholic University of Korea) ;
  • Ra, Kyung-Soo (Department of Food and Nutrition, Daegu Technical College) ;
  • Chang, In-Seop (Water Environment Research Center, Korea Institute of Science and Technology) ;
  • Park, Yoon-Chang (Division of Life Resources, College of Natural Resources, Daegu University) ;
  • Park, Hee-Sung (Department of Plant Genetic Engineering, Catholic University of Daegu) ;
  • Baik, Hyung-Suk (Department of Microbiology, Pusan National University) ;
  • Yun, Jong-Won (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Choi, Jang-Won (Division of Life Resources, College of Natural Resources, Daegu University)
  • Published : 2003.03.31
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Abstract

Hot water-soluble crude polysaccharide (HCAP-0) that was obtained from the fruits of Capsicum annuum showed potent anti-complementary activity. The activity was unchanged by pronase digestion, but decreased by periodate oxidation. The HCAP-0 was fractionated by DEAE ion-exchange chromatography to give two major fractions, HCAP-II and III. These two fractions were finally purified by gel filtration to give HCAP-IIa, HCAP-IIIa1, and IIIa2 fractions that had high anti-complementary activities. The HCAP-IIIa1 and IIIa2 consisted of homogeneous polysaccharides. The anti-complementary activities were unaffected by treatment with polymyxin B, indicating that the modes of complement activation were not due to preexisting lipopolysaccharide. The molecular weight and sugar content of HCAP-IIIa2 had potent anti-complementary activity. The highest yields were 55 kDa and 75.9%, and the molar ratio of galactose (Ara:Gal, 1.0:4.6) was higher than other sugars. The crossed immuno-electrophoresis showed that both classical and alternative pathways were activated by HCAP-IIIa2.

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References

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