Asian-Australasian Journal of Animal Sciences

  • 제16권7호
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  • Pages.1066-1070
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  • 2003
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Identification of Differentially Expressed Genes in the Longissimus Dorsi Muscle Tissue between Duroc and Erhualian Pigs by mRNA Differential Display

  • Pan, P.W. (Laboratory of Molecular Biology and Animal Breeding, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Zhao, S.H. (Laboratory of Molecular Biology and Animal Breeding, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Yu, M. (Laboratory of Molecular Biology and Animal Breeding, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Liu, B. (Laboratory of Molecular Biology and Animal Breeding, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Xiong, T.A. (Laboratory of Molecular Biology and Animal Breeding, College of Animal Science and Technology, Huazhong Agricultural University) ;
  • Li, K. (Laboratory of Molecular Biology and Animal Breeding, College of Animal Science and Technology, Huazhong Agricultural University)
  • 투고 : 2002.12.11
  • 심사 : 2003.03.17
  • 발행 : 2003.07.01
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초록

In order to identify differentially expressed mRNAs (which represent possible candidates for significant phenotypic variances of muscle growth, meat quality between introduced European and Chinese indigenous pigs) in the longissimus dorsi muscle tissue between adult Duroc and Erhualian pigs, mRNA differential display was performed. Five 3' anchor primers in combination with 20 different 5' arbitrary primers (100 primer sets) were used and nearly 5,000 cDNA bands were examined, among which 10 differential display cDNAs were obtained, cloned and sequenced. Six of the 10 cDNAs showed similarity to identified genes from GenBank and the other 4 had no matches in GenBank. Differential expression was tested by Northern blot hybridization and could be confirmed for 2 cDNAs. The method used in this study provides a useful molecular tool to investigate genetic variation that occurs at the transcriptional level between different breeds.

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참고문헌

  1. Bauer, D., H. Muller, J. Reich, H. Riedei, V. Ahrenkiel, P. Warthoe and M. Strauss. 1993. Identification of differentially expressed mRNA species by an improved display technique (DDRTPCR). Nucleic Acids Res. 21:4272-4280. https://doi.org/10.1093/nar/21.18.4272
  2. Chen, S. L., M. H. Li, Y. J. Li, S. H. Zhao, C. Z. Yu, M. Yu, B. Fan and K. Li. 2001. RAPD variation and genetic distance among Tibetan, Inner Mongolia and liaoning cashmere goats. Asian-Aust. J. Anim. Sci.14:1520-1522. https://doi.org/10.5713/ajas.2001.1520
  3. Davoli, R., P. Zambonelli, D. Bigi, L. Fontanesi and V. Russo. 1999. Analysis of expressed sequence tags of porcine skeletal muscle. Gene. 233:181-188. https://doi.org/10.1016/S0378-1119(99)00141-9
  4. Doss, R. P. 1996. Differential display without radioactivity-a modified procedure. BioTechniques 21:408-412.
  5. Janzen, M. A., D. L. Kuhlers, S. B. Jungst and C. F. Louis. 2000. ARPP-16 mRNA is up-regulated in the longissimus muscle of pigs possessing an elevated growth rate. J. Anim. Sci. 78: 1475-1484. https://doi.org/10.2527/2000.7861475x
  6. Johns, G. F. 1998. Genetic aspects of domestication, common breeds and their origin. In The genetics of the pig. (Ed. M. F. Rothschild and A. Ruvinsky). CAB INTERNATIONAL, Wallingford, Oxford, UK. pp.17-48.
  7. Lauren, S., J. Stephen, C. B. Timothy, D. G. Tenen and J. D. Kathleen. 1997. Overcoming limitations of the mRNA differential display technique. Nucleic Acids Res. 23:4738-4739. https://doi.org/10.1093/nar/23.22.4738
  8. Li, M. D., R. L. Matteri, G. J. Macdonald, T. H. Wise and J. J. Ford. 1996. Overexpression of $\beta$-subunit of thyroid-stimulating hormone in meishan swine identified by differential display. J. Anim. Sci. 74:2104-2111. https://doi.org/10.2527/1996.7492104x
  9. Liang, P. and A. B. Pardee. 1992. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257:967-971. https://doi.org/10.1126/science.1354393
  10. Liang, P., W. M. Zhu, X. Y. Zhang, Z. Guo, R. P. O'Connell, L. Averboukh, F. L. Wang and A. B. Pardee. 1994. Differential display using one-base anchored oligo-dT primers. Nucleic Acids Res. 22:5763-5764. https://doi.org/10.1093/nar/22.25.5763
  11. Neilan, B. A., D. A. Leigh, E. Rapley and B. L. McDonald. 1994. Microsatellite genome screening: rapid nondenaturing, nonisotopic dinucleotide repeat analysis. BioTechniques 17:708-712.
  12. Ponsuksili, S., K. Wimmers, F. Schmoll, A. Robic and K. Schellander. 2000. Porcine ESTs detected by differential display representing possible candidates for the trait 'eye muscle area'. J. Anim. Breed. Genet. 117:25-35. https://doi.org/10.1046/j.1439-0388.2000.00227.x
  13. Raikhinstein, M. and I. Hanukoglu. 1993. Mitochondrial-genomeencoded RNAs: differential regulation by corticotropin in bovine adrenocortical cells. Proc. Natl. Acad. Sci. USA. 90:10509-10513. https://doi.org/10.1073/pnas.90.22.10509
  14. Roger, R. and K. Gunter. 1997. mRNA differential display in agarose gels. BioTechniques 23:28-32.
  15. Sambrook J., E. F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. 2nd Ed. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York.

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