Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimicrobial Activity of Grape Seed Ethanol Extract

포도종실 에탄올 추출물의 항균 활성

  • 정하열 (한경대학교 식품공학과 및 식품생물산업연구소) ;
  • 박동규 (한경대학교 식품공학과 및 식품생물산업연구소)
  • Published : 2003.02.01
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Abstract

Antimicrobial activities of grape seed ethanol extract and its serial solvent fractions were investigated against various food poisoning microorganisms. The grape seed ethanol extract showed dose dependant antimicrobial activity against Bacillus subtilis ATCC 9372 or Staphylococcus aureus ATCC 6538, whereas had limited effect on Pseudomonas aeruginosa IFO 3080, Salmonella enteritidis IFO 3313 and Escherichia coli ATCC 25922. Ethylacetate and butanol fractions ameng the serial solvent fractions of grape seed ethanol extract contained the catechin at the levels of 35.7 mg/g and 20.2 mg/g, respectively. Nevertheless, the butanol fraction of grape seed ethanol extract showed intense antimicrobial activity compared with the ethylacetate fraction on all microorganisms tested. It was found that the butanol fraction was mainly composed of oligomeric or polymeric polyphenols such as condensed tannins by the fractionation on C l8 cartridge according to the difference in the degree of polymerization. Therefore, it seems that the antimicrobial activity of grape seed ethanol extract is related to the degree of polymerization of proanthocyanidin as well as the total content of flavan-3-ol composing the proanthocyanidin.

포도종실 에탄올 추출물의 각 검정 균주에 대한 생육 억제효과를 페이퍼디스크법 및 액체배양법에 의해 조사한 결과, B. subtilis ATCC 6538 혹은 S. aureus. ATCC 6538에 대하여는 농도 의존적인 증식억제 효과를 나타냈으며 P. aeruginosa IFO 3080 및 Sal. enteritidis. IFO3313에 대해서는 제한적인 증식억제 효과를 나타내기도 하였으나 E. coli ATCC 25922 에 대해서는 뚜렷한 항균 효과를 확인하기 어려웠다. 또한 포도종실 에탄을 추출물의 순차 용매 분획물 중에서 에틸아세테이트 분획과 부탄올 분획물만이 각각 35.7 mg/g과 20.2 mg/g의 카테킨을 함유하고 있었는데 에틸아세테이트 분획에 비하여 부탄을 분획물이 각 검정 균주에 대해 뚜렷한 항균활성 을 나타내었다. 부탄올 분획물은 에틸아세테이트 분획물에 비하여 카테킨의 절대 함량은 적었지만 Cl8 카트리지로 소분획하였을 때 고분자체 중심의 폴리페놀 화합물로 구성되어 있음을 알 수 있었다. 따라서 포도종실 에탄올 추출물 의 항균 활성은 카테킨과 같은 플라반-3-올 화합물의 절대 함량과 더불어 플라반-3-올 화합물의 중합도가 관계하는 것으로 예측되었다.

Keywords

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